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Special Issue "Nucleic Acid Aptamers"

A special issue of Molecules (ISSN 1420-3049). This special issue belongs to the section "Bioorganic Chemistry".

Deadline for manuscript submissions: 10 March 2018

Special Issue Editor

Guest Editor
Prof. Masayasu Kuwahara

Graduate School of Science and Technology, Gunma University, 1-5-1 Tenjin-cho, Kiryu, Japan
Website | E-Mail
Interests: bioanalytical chemistry; nucleic acid aptamers; xenonucleic acids; polymerases

Special Issue Information

Dear Colleagues,

Nucleic acid aptamers are DNA or RNA molecules that can specifically bind to their targets to form stable target/aptamer complexes. Since the establishment of the systematic evolution of ligands by exponential enrichment method around 1990, nucleic acid aptamers for various targets have been developed. Numerous improved methodologies for their selections and various applications, such as bioimaging, diagnoses, molecular therapies, logic gates, and DNA origami technology, have been reported to date. To achieve improved binding affinity and specificity, increased in vivo stability, and further expanded applications, computational predictions, engineering, and chemical modifications have also been attempted. Among these, some attempts have been successful in efficient acquisition of high affinity aptamers and practical uses. However, technical issues still remain in aptamer development. The present Special Issue is aimed at covering a new aspect of research and development pertaining to nucleic acid aptamers. For example, papers regarding novel methodologies for selections and structural/functional characterizations, newly acquired aptamers, engineering/modification strategies, in silico studies, and diverse applications, as well as related elemental technologies and materials for the potential uses will be invited.

Prof. Masayasu Kuwahara
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All papers will be peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Molecules is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 1800 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • aptamers
  • riboswitches
  • xenonucleic acids
  • polymerase variants
  • therapeutic and diagnositic nucleic acids
  • biosensors

Published Papers (1 paper)

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Research

Open AccessCommunication In Vitro Selection of DNA Aptamers that Binds Geniposide
Molecules 2017, 22(3), 383; doi:10.3390/molecules22030383
Received: 26 January 2017 / Accepted: 21 February 2017 / Published: 28 February 2017
PDF Full-text (5184 KB) | HTML Full-text | XML Full-text | Supplementary Files
Abstract
Geniposide is a key iridoid glycoside from Gardenia jasminoides fructus widely used in traditional Chinese herbal medicine. However, detection of this small molecule represents a significant challenge mostly due to the lack of specific molecular recognition elements. In this study, we have performed
[...] Read more.
Geniposide is a key iridoid glycoside from Gardenia jasminoides fructus widely used in traditional Chinese herbal medicine. However, detection of this small molecule represents a significant challenge mostly due to the lack of specific molecular recognition elements. In this study, we have performed in vitro selection experiments to isolate DNA aptamers that can specifically bind geniposide. Using a stringent selection procedure, we have isolated DNA aptamers that can distinguish geniposide from genipin and glucose, two structural analogs of geniposide. Two top aptamers exhibit low micromolar binding affinity towards geniposide, but show significantly reduced affinity to genipin and glucose. These aptamers have the potential to be further developed into analytical tools for the detection of geniposide. Full article
(This article belongs to the Special Issue Nucleic Acid Aptamers)
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