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Molecules 2018, 23(2), 291; doi:10.3390/molecules23020291

A Lateral Flow Strip Based Aptasensor for Detection of Ochratoxin A in Corn Samples

1
Key Laboratory of Agro-Product Quality and Safety, Institute of Quality Standards and Testing Technology for Agro-Products, Chinese Academy of Agricultural Sciences, Beijing 100081, China
2
College of Food Science and Technology, Hainan University, Haikou 570228, China
*
Author to whom correspondence should be addressed.
Received: 19 December 2017 / Revised: 11 January 2018 / Accepted: 15 January 2018 / Published: 31 January 2018
(This article belongs to the Special Issue Nucleic Acid Aptamers)
View Full-Text   |   Download PDF [1640 KB, uploaded 31 January 2018]   |  

Abstract

Ochratoxin A (OTA) is a mycotoxin identified as a contaminant in grains and wine throughout the world, and convenient, rapid and sensitive detection methods for OTA have been a long-felt need for food safety monitoring. Herein, we presented a new competitive format based lateral flow strip fluorescent aptasensor for one-step determination of OTA in corn samples. Briefly, biotin-cDNA was immobilized on the surface of a nitrocellulose filter on the test line. Without OTA, Cy5-labeled aptamer combined with complementary strands formed a stable double helix. In the presence of OTA, however, the Cy5-aptamer/OTA complexes were generated, and therefore less free aptamer was captured in the test zone, leading to an obvious decrease in fluorescent signals on the test line. The test strip showed an excellent linear relationship in the range from 1 ng·mL−1 to 1000 ng·mL−1 with the LOD of 0.40 ng·mL−1, IC15 value of 3.46 ng·mL−1 and recoveries from 96.4% to 104.67% in spiked corn samples. Thus, the strip sensor developed in this study is an acceptable alternative for rapid detection of the OTA level in grain samples. View Full-Text
Keywords: aptamer; lateral flow strip; detection; Ochratoxin A; corn samples aptamer; lateral flow strip; detection; Ochratoxin A; corn samples
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Zhang, G.; Zhu, C.; Huang, Y.; Yan, J.; Chen, A. A Lateral Flow Strip Based Aptasensor for Detection of Ochratoxin A in Corn Samples. Molecules 2018, 23, 291.

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