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Molecules 2017, 22(12), 2070; doi:10.3390/molecules22122070

Development of Cell-Specific Aptamers: Recent Advances and Insight into the Selection Procedures

1
Centre for comparative Genomics, Murdoch University, Perth 6150, Australia
2
Perron Institute for Neurological and Translational Science, Perth 6005, Australia
3
School of Medicine, Deakin University, Waurn Ponds, Geelong 3216, Australia
*
Author to whom correspondence should be addressed.
Received: 7 November 2017 / Revised: 24 November 2017 / Accepted: 24 November 2017 / Published: 27 November 2017
(This article belongs to the Special Issue Nucleic Acid Aptamers)
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Abstract

Systematic evolution of ligands by exponential enrichment (SELEX) is an established procedure for developing short single-stranded nucleic acid ligands called aptamers against a target of choice. This approach has also been used for developing aptamers specific to whole cells named Cell-SELEX. Aptamers selected by Cell-SELEX have the potential to act as cell specific therapeutics, cell specific markers or cell specific drug delivery and imaging agents. However, aptamer development is a laborious and time-consuming process which is often challenging due to the requirement of frequent optimization of various steps involved in Cell-SELEX procedures. This review provides an insight into various procedures for selection, aptamer enrichment, regeneration and aptamer-binding analysis, in addition to a very recent update on all aptamers selected by Cell-SELEX procedures. View Full-Text
Keywords: aptamers; Cell-SELEX; aptamer enrichment; ssDNA; binding assays aptamers; Cell-SELEX; aptamer enrichment; ssDNA; binding assays
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Rahimizadeh, K.; AlShamaileh, H.; Fratini, M.; Chakravarthy, M.; Stephen, M.; Shigdar, S.; Veedu, R.N. Development of Cell-Specific Aptamers: Recent Advances and Insight into the Selection Procedures. Molecules 2017, 22, 2070.

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