Development of Cell-Specific Aptamers: Recent Advances and Insight into the Selection Procedures
AbstractSystematic evolution of ligands by exponential enrichment (SELEX) is an established procedure for developing short single-stranded nucleic acid ligands called aptamers against a target of choice. This approach has also been used for developing aptamers specific to whole cells named Cell-SELEX. Aptamers selected by Cell-SELEX have the potential to act as cell specific therapeutics, cell specific markers or cell specific drug delivery and imaging agents. However, aptamer development is a laborious and time-consuming process which is often challenging due to the requirement of frequent optimization of various steps involved in Cell-SELEX procedures. This review provides an insight into various procedures for selection, aptamer enrichment, regeneration and aptamer-binding analysis, in addition to a very recent update on all aptamers selected by Cell-SELEX procedures. View Full-Text
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Rahimizadeh, K.; AlShamaileh, H.; Fratini, M.; Chakravarthy, M.; Stephen, M.; Shigdar, S.; Veedu, R.N. Development of Cell-Specific Aptamers: Recent Advances and Insight into the Selection Procedures. Molecules 2017, 22, 2070.
Rahimizadeh K, AlShamaileh H, Fratini M, Chakravarthy M, Stephen M, Shigdar S, Veedu RN. Development of Cell-Specific Aptamers: Recent Advances and Insight into the Selection Procedures. Molecules. 2017; 22(12):2070.Chicago/Turabian Style
Rahimizadeh, Kamal; AlShamaileh, Hadi; Fratini, Milena; Chakravarthy, Madhuri; Stephen, Michelle; Shigdar, Sarah; Veedu, Rakesh N. 2017. "Development of Cell-Specific Aptamers: Recent Advances and Insight into the Selection Procedures." Molecules 22, no. 12: 2070.
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