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Molecules 2017, 22(3), 383; doi:10.3390/molecules22030383

In Vitro Selection of DNA Aptamers that Binds Geniposide

1
School of Basic Medical Science, Beijing University of Chinese Medicine, 11 East Road, North 3rd Ring Road, Chaoyang District, Beijing 100029, China
2
Department of Biochemistry and Biomedical Sciences, McMaster University, 1280 Main Street West, Hamilton, ON, L8S 4K1, Canada
3
Beijing Institute of Traditional Chinese Medicine, Beijing University of Chinese Medicine, 11 East Road, North 3rd Ring Road, Chaoyang District, Beijing 100029, China
*
Author to whom correspondence should be addressed.
Academic Editor: Masayasu Kuwahara
Received: 26 January 2017 / Accepted: 21 February 2017 / Published: 28 February 2017
(This article belongs to the Special Issue Nucleic Acid Aptamers)
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Abstract

Geniposide is a key iridoid glycoside from Gardenia jasminoides fructus widely used in traditional Chinese herbal medicine. However, detection of this small molecule represents a significant challenge mostly due to the lack of specific molecular recognition elements. In this study, we have performed in vitro selection experiments to isolate DNA aptamers that can specifically bind geniposide. Using a stringent selection procedure, we have isolated DNA aptamers that can distinguish geniposide from genipin and glucose, two structural analogs of geniposide. Two top aptamers exhibit low micromolar binding affinity towards geniposide, but show significantly reduced affinity to genipin and glucose. These aptamers have the potential to be further developed into analytical tools for the detection of geniposide. View Full-Text
Keywords: geniposide; aptamer; in vitro selection; DNA; Chinese herbal medicine; molecular recognition geniposide; aptamer; in vitro selection; DNA; Chinese herbal medicine; molecular recognition
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Zhang, A.; Chang, D.; Zhang, Z.; Li, F.; Li, W.; Wang, X.; Li, Y.; Hua, Q. In Vitro Selection of DNA Aptamers that Binds Geniposide. Molecules 2017, 22, 383.

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