Special Issue "ELISPOT Research"

Quicklinks

A special issue of Cells (ISSN 2073-4409).

Deadline for manuscript submissions: closed (20 July 2015)

Special Issue Editor

Guest Editor
Dr. Alexander E. Kalyuzhny
Neuroscience, UMN Twin Cities, 6-145 Jackson Hall, 321 Church St SE, Minneapolis, MN 55455, USA
Website: http://www.tc.umn.edu/~kalyu001/
Phone: +1 612 624 2991
Interests: physiology of pain; antinociceptive brainstem circuit; cellular localization, trafficking and oligomerization of opioid receptors; drugs of abuse; cytokines and cytokine receptors

Special Issue Information

Dear Colleagues,

ELISPOT is a technique of choice for the detection of spontaneous and antigen-induced secretion of cytokines (e.g. IFN-gamma, TNF-alpha, IL-2, IL-4, etc.) from peripheral blood lymphocytes. This assay is widely used for vaccine development, AIDS research, cancer research, infectious diseases monitoring, autoimmune disease studies, and allergy and transplantation research. Due to its unsurpassed sensitivity, the areas of ELISPOT application are rapidly growing and expanding into human and veterinary diagnostics, vaccine development, drug screening, and as a companion assay for high content screening. The growing number of publications attests towards the recognition that ELISPOT technique is an indispensable tool for both basic research and diagnostic studies and is strong evidence that this technique is a must-use tool in modern day biomedical research. Due to its inherent nature, ELISPOT is one of the most flexible assays and can be endlessly modified and adapted to particular experimental needs of the investigator. This Special Issue offers an Open Access forum at bringing together a collection of novel protocols, methods and techniques that can be shared among investigators from different laboratories. We encourage novices and experienced scientists to contribute research papers and reviews dedicated to analysis of secretion of novel cytokines, multiplex ELSIPOT techniques using chromogenic and fluorescence detection, statistical analysis, novel cell-treatment strategies, high-sensitivity detection protocols, T-cell and B-cell ELISPOT assays, vaccine development, diagnostics and drug discovery research. ELISPOT has enormous potential for development and expansion into new research areas and our goal is to help advancing in this direction.

Dr. Alexander E. Kalyuzhny
Guest Editor

Submission

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. Papers will be published continuously (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are refereed through a peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Cells is an international peer-reviewed Open Access quarterly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 500 CHF (Swiss Francs). English correction and/or formatting fees of 250 CHF (Swiss Francs) will be charged in certain cases for those articles accepted for publication that require extensive additional formatting and/or English corrections.


Keywords

  • ELISPOT
  • fluorospot
  • multiplexing
  • T-cell ELISPOT
  • B-cell ELISPOT
  • vaccine development
  • cancer research
  • infection disease
  • diagnostics
  • drug discovery
  • statistical analysis
  • high-sensitivity detection

Published Papers (25 papers)

Download All Papers
Sort by:
Display options:
Select articles Export citation of selected articles as:
Select/unselect all
Displaying article 1-25
p. 133-134
by , , , , , , ,  and
Cells 2015, 4(2), 133-134; doi:10.3390/cells4020133
Received: 16 April 2015 / Accepted: 20 April 2015 / Published: 21 April 2015
Show/Hide Abstract | PDF Full-text (633 KB) | HTML Full-text | XML Full-text
(This article belongs to the Special Issue ELISPOT Research)
p. 96-111
by , ,  and
Cells 2015, 4(1), 96-111; doi:10.3390/cells4010096
Received: 23 October 2014 / Revised: 12 January 2015 / Accepted: 16 January 2015 / Published: 2 March 2015
Show/Hide Abstract | PDF Full-text (1530 KB) | HTML Full-text | XML Full-text
(This article belongs to the Special Issue ELISPOT Research)
p. 84-95
by , , , , ,  and
Cells 2015, 4(1), 84-95; doi:10.3390/cells4010084
Received: 31 October 2014 / Accepted: 30 January 2015 / Published: 11 February 2015
Show/Hide Abstract | PDF Full-text (1037 KB) | HTML Full-text | XML Full-text
(This article belongs to the Special Issue ELISPOT Research)
p. 71-83
by , , , ,  and
Cells 2015, 4(1), 71-83; doi:10.3390/cells4010071
Received: 30 October 2014 / Revised: 18 November 2014 / Accepted: 1 December 2014 / Published: 29 January 2015
Show/Hide Abstract | PDF Full-text (2518 KB) | HTML Full-text | XML Full-text
(This article belongs to the Special Issue ELISPOT Research)
p. 56-70
by , , , , , ,  and
Cells 2015, 4(1), 56-70; doi:10.3390/cells4010056
Received: 3 November 2014 / Accepted: 14 January 2015 / Published: 20 January 2015
Show/Hide Abstract | PDF Full-text (1699 KB) | HTML Full-text | XML Full-text
(This article belongs to the Special Issue ELISPOT Research)
p. 21-39
by , , , , , , , , , , , ,  and
Cells 2015, 4(1), 21-39; doi:10.3390/cells4010021
Received: 24 October 2014 / Accepted: 26 November 2014 / Published: 9 January 2015
Show/Hide Abstract | PDF Full-text (1830 KB) | HTML Full-text | XML Full-text
(This article belongs to the Special Issue ELISPOT Research)
p. 40-55
by , , ,  and
Cells 2015, 4(1), 40-55; doi:10.3390/cells4010040
Received: 29 October 2014 / Accepted: 5 December 2014 / Published: 9 January 2015
Show/Hide Abstract | PDF Full-text (1194 KB) | HTML Full-text | XML Full-text | Supplementary Files
(This article belongs to the Special Issue ELISPOT Research)
p. 1-18
by , , , , , , ,  and
Cells 2015, 4(1), 1-18; doi:10.3390/cells4010001
Received: 9 October 2014 / Accepted: 16 December 2014 / Published: 24 December 2014
Show/Hide Abstract | Cited by 2 | PDF Full-text (2628 KB) | HTML Full-text | XML Full-textCorrection | Supplementary Files
(This article belongs to the Special Issue ELISPOT Research)
p. 1102-1115
by ,  and
Cells 2014, 3(4), 1102-1115; doi:10.3390/cells3041102
Received: 8 October 2014 / Revised: 13 November 2014 / Accepted: 14 November 2014 / Published: 27 November 2014
Show/Hide Abstract | Cited by 3 | PDF Full-text (3695 KB) | HTML Full-text | XML Full-text
(This article belongs to the Special Issue ELISPOT Research)
p. 1116-1130
by , ,  and
Cells 2014, 3(4), 1116-1130; doi:10.3390/cells3041116
Received: 15 October 2014 / Revised: 10 November 2014 / Accepted: 21 November 2014 / Published: 27 November 2014
Show/Hide Abstract | Cited by 2 | PDF Full-text (2778 KB) | HTML Full-text | XML Full-text
(This article belongs to the Special Issue ELISPOT Research)
abstract graphic
p. 19-42
by , , , , , , , ,  and
Cells 2013, 2(1), 19-42; doi:10.3390/cells2010019
Received: 19 October 2012 / Revised: 22 November 2012 / Accepted: 27 December 2012 / Published: 4 January 2013
Show/Hide Abstract | PDF Full-text (716 KB) | HTML Full-text | XML Full-text
(This article belongs to the Special Issue ELISPOT Research)
p. 576-596
by , ,  and
Cells 2012, 1(3), 576-596; doi:10.3390/cells1030576
Received: 16 July 2012 / Revised: 28 July 2012 / Accepted: 31 July 2012 / Published: 24 August 2012
Show/Hide Abstract | PDF Full-text (591 KB) | HTML Full-text | XML Full-text
(This article belongs to the Special Issue ELISPOT Research)
abstract graphic
p. 409-427
by , , , , ,  and
Cells 2012, 1(3), 409-427; doi:10.3390/cells1030409
Received: 13 June 2012 / Revised: 12 July 2012 / Accepted: 14 July 2012 / Published: 30 July 2012
Show/Hide Abstract | Cited by 6 | PDF Full-text (710 KB) | HTML Full-text | XML Full-text
(This article belongs to the Special Issue ELISPOT Research)
p. 153-167
by , , , , , ,  and
Cells 2012, 1(2), 153-167; doi:10.3390/cells1020153
Received: 1 April 2012 / Revised: 7 May 2012 / Accepted: 8 May 2012 / Published: 8 June 2012
Show/Hide Abstract | Cited by 2 | PDF Full-text (123 KB) | HTML Full-text | XML Full-text
(This article belongs to the Special Issue ELISPOT Research)
p. 141-152
by
Cells 2012, 1(2), 141-152; doi:10.3390/cells1020141
Received: 15 April 2012 / Revised: 17 May 2012 / Accepted: 22 May 2012 / Published: 29 May 2012
Show/Hide Abstract | PDF Full-text (167 KB) | HTML Full-text | XML Full-text
(This article belongs to the Special Issue ELISPOT Research)
p. 100-110
by  and
Cells 2012, 1(2), 100-110; doi:10.3390/cells1020100
Received: 23 March 2012 / Revised: 30 April 2012 / Accepted: 7 May 2012 / Published: 10 May 2012
Show/Hide Abstract | PDF Full-text (165 KB) | HTML Full-text | XML Full-text
(This article belongs to the Special Issue ELISPOT Research)
p. 111-126
by , , ,  and
Cells 2012, 1(2), 111-126; doi:10.3390/cells1020111
Received: 28 March 2012 / Revised: 30 April 2012 / Accepted: 7 May 2012 / Published: 10 May 2012
Show/Hide Abstract | Cited by 4 | PDF Full-text (104 KB) | HTML Full-text | XML Full-text
(This article belongs to the Special Issue ELISPOT Research)
p. 127-140
by , , , , ,  and
Cells 2012, 1(2), 127-140; doi:10.3390/cells1020127
Received: 6 April 2012 / Revised: 30 April 2012 / Accepted: 7 May 2012 / Published: 10 May 2012
Show/Hide Abstract | PDF Full-text (365 KB) | HTML Full-text | XML Full-text
(This article belongs to the Special Issue ELISPOT Research)
p. 89-99
by ,  and
Cells 2012, 1(2), 89-99; doi:10.3390/cells1020089
Received: 14 March 2012 / Revised: 11 April 2012 / Accepted: 17 April 2012 / Published: 7 May 2012
Show/Hide Abstract | PDF Full-text (200 KB) | HTML Full-text | XML Full-text
(This article belongs to the Special Issue ELISPOT Research)
p. 61-73
by ,  and
Cells 2012, 1(2), 61-73; doi:10.3390/cells1020061
Received: 29 March 2012 / Revised: 24 April 2012 / Accepted: 26 April 2012 / Published: 30 April 2012
Show/Hide Abstract | PDF Full-text (214 KB) | HTML Full-text | XML Full-text
(This article belongs to the Special Issue ELISPOT Research)
p. 51-60
by , , ,  and
Cells 2012, 1(2), 51-60; doi:10.3390/cells1020051
Received: 26 February 2012 / Revised: 7 March 2012 / Accepted: 3 April 2012 / Published: 26 April 2012
Show/Hide Abstract | PDF Full-text (344 KB) | HTML Full-text | XML Full-text
(This article belongs to the Special Issue ELISPOT Research)
p. 35-50
by , , , ,  and
Cells 2012, 1(2), 35-50; doi:10.3390/cells1020035
Received: 22 March 2012 / Revised: 12 April 2012 / Accepted: 16 April 2012 / Published: 23 April 2012
Show/Hide Abstract | Cited by 1 | PDF Full-text (1142 KB) | HTML Full-text | XML Full-text
(This article belongs to the Special Issue ELISPOT Research)
p. 27-34
by , ,  and
Cells 2012, 1(2), 27-34; doi:10.3390/cells1020027
Received: 13 March 2012 / Revised: 4 April 2012 / Accepted: 6 April 2012 / Published: 20 April 2012
Show/Hide Abstract | PDF Full-text (172 KB) | HTML Full-text | XML Full-text
(This article belongs to the Special Issue ELISPOT Research)
p. 15-26
by  and
Cells 2012, 1(1), 15-26; doi:10.3390/cells1010015
Received: 1 March 2012 / Revised: 15 March 2012 / Accepted: 16 March 2012 / Published: 21 March 2012
Show/Hide Abstract | PDF Full-text (162 KB) | HTML Full-text | XML Full-text
(This article belongs to the Special Issue ELISPOT Research)
p. 5-14
by , , , , ,  and
Cells 2012, 1(1), 5-14; doi:10.3390/cells1010005
Received: 9 February 2012 / Revised: 28 February 2012 / Accepted: 6 March 2012 / Published: 13 March 2012
Show/Hide Abstract | PDF Full-text (236 KB) | HTML Full-text | XML Full-text
(This article belongs to the Special Issue ELISPOT Research)
Select/unselect all
Displaying article 1-25
Select articles Export citation of selected articles as:

Last update: 16 March 2015

Cells EISSN 2073-4409 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert