Next Article in Journal / Special Issue
Triple Cytokine FluoroSpot Analysis of Human Antigen-Specific IFN-γ, IL-17A and IL-22 Responses
Previous Article in Journal
Natural and Synthetic Modulators of the TRPM7 Channel
Previous Article in Special Issue
CD8+ T Lymphocyte Epitopes From The Herpes Simplex Virus Type 2 ICP27, VP22 and VP13/14 Proteins To Facilitate Vaccine Design And Characterization
Article Menu

Export Article

Open AccessArticle
Cells 2014, 3(4), 1102-1115; doi:10.3390/cells3041102

Stepping up ELISpot: Multi-Level Analysis in FluoroSpot Assays

1
ZellNet Consulting, Inc., 555 North Avenue, Suite 25-S, Fort Lee, NJ 07024, USA
2
Autoimmun Diagnostika GmbH, Ebinger Strasse 4, D-72479 Strassberg, Germany
3
Mabtech AB, Box 1233, SE-131 28 Nacka Strand, Sweden
*
Author to whom correspondence should be addressed.
Received: 8 October 2014 / Revised: 13 November 2014 / Accepted: 14 November 2014 / Published: 27 November 2014
(This article belongs to the Special Issue ELISPOT Research)
View Full-Text   |   Download PDF [3695 KB, uploaded 27 November 2014]   |  

Abstract

ELISpot is one of the most commonly used immune monitoring assays, which allows the functional assessment of the immune system at the single cell level. With its outstanding sensitivity and ease of performance, the assay has recently advanced from the mere single function cell analysis to multifunctional analysis by implementing detection reagents that are labeled with fluorophores (FluoroSpot), allowing the detection of secretion patterns of two or more analytes in a single well. However, the automated evaluation of such assays presents various challenges for image analysis. Here we dissect the technical and methodological requirements for a reliable analysis of FluoroSpot assays, introduce important quality control measures and provide advice for proper interpretation of results obtained by automated imaging systems. View Full-Text
Keywords: FluoroSpot; ELISpot; immunomonitoring; image analysis; fluorochromes; fluorophores FluoroSpot; ELISpot; immunomonitoring; image analysis; fluorochromes; fluorophores
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Janetzki, S.; Rueger, M.; Dillenbeck, T. Stepping up ELISpot: Multi-Level Analysis in FluoroSpot Assays. Cells 2014, 3, 1102-1115.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Cells EISSN 2073-4409 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top