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Cells 2012, 1(2), 141-152; doi:10.3390/cells1020141

Enzyme Linked Immuno-Spot; a Useful Tool in the Search for Elusive Immune Markers in Common Pediatric Immunological Diseases

The Biomedical Platform, Department of Natural Science and Biomedicine, School of Health Sciences, Jönköping University and County Hospital, Ryhov, Jönköping S-551 11, Sweden
Received: 15 April 2012 / Revised: 17 May 2012 / Accepted: 22 May 2012 / Published: 29 May 2012
(This article belongs to the Special Issue ELISPOT Research)
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Abstract

In order to provide better therapy we strive to increase our knowledge of how the immune system behaves and communicates in common pediatric immunological diseases, such as type 1 diabetes, allergic and celiac diseases. However, when dealing with pediatric diseases, where study subjects are almost exclusively children, blood volumes available for immunological studies are limited and as such must be carefully handled and used to their full extent. Single immune markers can easily be detected by a traditional Enzyme Linked Immunosorbent Assay (ELISA), whereas multiple markers can be detected by a fluorochrome (Luminex) or electrochemiluminescence (MSD) technique. These techniques however are sometimes not sensitive enough to detect low levels of secreted immune markers in limited sample sizes. To detect immune markers at the single-cell level, an Enzyme Linked Immuno-spot (ELISPOT) can be used to pin-point elusive immune markers in common pediatric immunological diseases. View Full-Text
Keywords: Enzyme Linked Immuno-spot (ELISPOT); pediatric immunological diseases; immune markers; cytokines Enzyme Linked Immuno-spot (ELISPOT); pediatric immunological diseases; immune markers; cytokines
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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Faresjö, M. Enzyme Linked Immuno-Spot; a Useful Tool in the Search for Elusive Immune Markers in Common Pediatric Immunological Diseases. Cells 2012, 1, 141-152.

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