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Cells 2012, 1(2), 153-167; doi:10.3390/cells1020153
Article

Can ELISPOT Be Applied to A Clinical Setting as A Diagnostic Utility for Neuroborreliosis?

1,2,* , 1,3
, 2
, 4
, 2
, 5,6
, 7
 and 5
1 Department of Clinical and Experimental Medicine, Infectious Medicine Faculty of Health Sciences, Linköping University, 581 83 Linköping, Sweden 2 The Åland Borrelia Group, Åland, 22100 Mariehamn, Åland, Finland 3 Department of Infectious Diseases, County Council of Östergötland, 581 85 Linköping, Sweden 4 Center for Clinical Research (CKF) Dalarna, 791 36 Falun, Sweden 5 Department of Clinical and Experimental Medicine, Clinical Immunology, Linköping University, S-581 83 Linköping, Sweden 6 Department of Clinical Immunology and Transfusion Medicine, County Council of Östergötland, 581 85 Linköping, Sweden 7 Department of Laboratory Medicine, NÄL, 461 85 Trollhättan, Sweden
* Author to whom correspondence should be addressed.
Received: 1 April 2012 / Revised: 7 May 2012 / Accepted: 8 May 2012 / Published: 8 June 2012
(This article belongs to the Special Issue ELISPOT Research)
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Abstract

The aim of this prospective study was to investigate the diagnostic performance of Borrelia (Bb)-induced interferon (IFN)-γ secretion detected by ELISPOT modified to be feasible for clinical laboratories as a supplementary test to the laboratory diagnosis of Lyme neuroborreliosis (LNB) in an endemic setting. Between 2002 and 2004, patients with symptoms of suspected clinical LNB were included in a study conducted on the Åland islands in the Finnish archipelago, which is a hyper-endemic area for Lyme borreliosis (LB). Fourteen patients with confirmed LNB and 103 patients with non-LNB were included, and the numbers of spontaneous and Bb-induced IFN-γ-secreting cells were assayed by the ELISPOT test. The ELISPOT assay showed a weak diagnostic performance with a sensitivity of 36% and a specificity of 82%. The findings in this study show that this ELISPOT-assay modified to be feasible in clinical routine laboratories is not useful as a supplementary diagnostic tool in the laboratory diagnosis of patients with clinically suspected LNB.
Keywords: Borrelia burgdorferi; neuroborreliosis; ELISPOT; cerebrospinal fluid; diagnostic test; sensitivity; specificity Borrelia burgdorferi; neuroborreliosis; ELISPOT; cerebrospinal fluid; diagnostic test; sensitivity; specificity
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Nordberg, M.; Forsberg, P.; Nyman, D.; Skogman, B.H.; Nyberg, C.; Ernerudh, J.; Eliasson, I.; Ekerfelt, C. Can ELISPOT Be Applied to A Clinical Setting as A Diagnostic Utility for Neuroborreliosis? Cells 2012, 1, 153-167.

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