Special Issue "Pulses"
Deadline for manuscript submissions: closed (31 December 2016)
Prof. Dr. Marcello Iriti
Department of Agricultural and Environmental Sciences, Faculty of Agricultural and Food Sciences, Milan State University, Via G. Celoria 2, Milan 20133, Italy
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Interests: bioactive phytochemicals; plant secondary metabolism; medicinal and food plants; environmental pollution; environmental stresses; nutraceuticals; functional foods; ethnobotany; complementary and alternative medicine
As you very probably know, 2016 is the International Year of Pulses, declared during the 68th United Nations General Assembly (A/RES/68/231), which aims to heighten public awareness of the nutritional benefits of pulses as relevant and indispensable component of a healthy dietary style.
Pulses belong to the subfamily Faboideae, included in the Fabaceae family, and represented an essential part of the human diet for centuries (the cutivation of some legumes dates back to 7000–8000 BC). Pulses also play an important role for sustainability: they are a main component of crop rotations, they require less fertilisers than other crops and they are a low carbon source of protein. Indeed, because of their nitrogen-fixing propertied, legumes are part of the rotational crops farmers can use to maintain soil fertility. In addition, pulses are a protein source with a low footprint, in both carbon and water, thus improving sustainable food production and indirectly reducing greenhouse gas emission. In nutrition, pulses are part of a healthy, balanced diet and have been shown to have an important role in preventing chronic-degenerative diseases such as certain types of cancer, diabetes and heart disease. Therefore, they can be fully considered a ‘superfood’, source of protein, dietary fibre, minerals, vitamins, and bioactive phytochemicals, with low glycaemic index, gluten-free and zero cholesterol. Importantly, legumes are model organisms in botany and plant molecular biology, such as, for instance, Lotus and Medicago, used to study the symbiosis responsible for nitrogen fixation.
In this very wide context, we invite investigators to submit both original research and review articles that explore all these aspects. Potential topics include, but are not limited to:
- Molecular Plant Biology
- Plant Disease
- Crop Production
- Environmental Pollution
- Abiotic Stresses
- Food Science
Professor Marcello Iriti
Manuscript Submission Information
Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All papers will be peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.
Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. International Journal of Molecular Sciences is an international peer-reviewed open access monthly journal published by MDPI.
Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 1800 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.
- functional foods
- plant foods
- Mediterranean diet
The below list represents only planned manuscripts. Some of these manuscripts have not been received by the Editorial Office yet. Papers submitted to MDPI journals are subject to peer-review.
Title: Comparative transcriptomic analysis of seed coat in relation to pea domestication
Authors: Hradilová Iveta, Trněný Oldřich, Krezdorn Nicolas, Khan W. Aamir, Varshney Rajeev, Winter Peter,Smýkal Petr
Abstract: Seed germination determines when plants begin growth in natural or agricultural ecosystems. In the wild, many seeds exhibit dormancy. In contrast, crops germinate as soon as they are imbibed usually at planting time. Physical dormancy involves the development of a water-impermeable seed coat. The objective of this study is to identify genetic basis of pea seed dormancy, one of the key domestication trait. Four parental contrasting genotypes were selected for comparative transcriptomic analysis. These included wild dormant P. elatius (JI64, VIR320) and cultivated P. sativum non-dormant (JI92, cv. Cameor). In addition two bulks (phenotypically assessed as dormant or non-dormant) of recombinant inbred lines (RILs) from JI64 x JI92 cross were used. Massive analysis of cDNA Ends (MACE) approach was applied on pooled sample from several developmental stages of isolated seed coat, resulting in over 10 million reads per sample. The reference sequences consisted of all Pisum mRNA sequences from NCBI and annotated to all Fabaceae proteins from Uniprot, by BLASTX to Swissprot and afterwards to Trembl protein databases. Normalization and test for differential gene expression between the bulks were calculated using the DEGSeq R/Bioconductor package. Gene Ontology enrichment analysis was performed by GenXPro available at www.tools.genxpro.net. GO-terms enrichment was calculated by the Fisher exact test based on transcripts that were differentially expressed at log2 higher than 2 and p-value of less than or equal to 0.05. Bioinformatic analysis resulted in identification of 976 differentially expressed genes between wild and cultivated genotypes. Of these the expression of 20 selected genes was studied by qRT-PCR at two developmental stages in four parental genotypes. The results will be shown and discussed in light of seed dormancy as well as domestication transition.
Title: Analysis of large seeds from three different Medicago truncatula ecotypes reveals a potential role of hormonal balance in final size determination of legume grains.
Authors: Kaustav Bandyopadhyay, Orhan Uluçay, Michael K. Udvardi, Muhammet Şakiroğlu and Jerome Verdier
Abstract: Legume seeds are an important protein and oil source for human diet and understanding how leguminous seeds develop and how their final seed size is determined are crucial economical questions in order to improve crop seed yield. In this study, we analysed seed development of three accessions of the model legume, Medicago truncatula, displaying contrasted phenotypes in seed size. By comparing two large seed accessions to the reference accession A17, we described mechanisms associated with large seed size determination to understand factors modulating the final seed size. We observed that early events during embryogenesis had an impact on final seed size and the large seed accessions resulted from a delayed embryo development to reach the heart stage. We also observed that the difference in seed growth rate was mainly due to a difference in embryo cell number, implicating a role of cell division rate. The higher cell number in large seed accessions could be explained by an extended period of cell division due to a longer embryogenesis phase. According to our observations and recent reports, we hypothesized that IAA and ABA ratio could be a key determinant of this process, probably acting as ON and OFF switches for cell division and entry into maturation phase. Overall, our study highlights that timing of events occurring during early seed development play decisive role for final seed size determination.