Next Article in Journal
MicroRNA-223-3p Regulates Ovarian Cancer Cell Proliferation and Invasion by Targeting SOX11 Expression
Previous Article in Journal
The Functions of MicroRNA-200 Family in Ovarian Cancer: Beyond Epithelial-Mesenchymal Transition
Previous Article in Special Issue
Meta-Analyses of QTLs Associated with Protein and Oil Contents and Compositions in Soybean [Glycine max (L.) Merr.] Seed
Article Menu
Issue 6 (June) cover image

Export Article

Open AccessArticle
Int. J. Mol. Sci. 2017, 18(6), 1205; doi:10.3390/ijms18061205

Genetic Variation Controlling Wrinkled Seed Phenotypes in Pisum: How Lucky Was Mendel?

1
John Innes Centre, Norwich Research Park, Norwich NR4 7UH, UK
2
IDna Genetics Ltd, Centrum, Norwich Research Park, Norwich NR4 7UG, UK
3
Department of Biology Sciences, University of Auckland, Auckland 1142, New Zealand
4
Department of Crop Physiology, International Centre for Agricultural Research in the Dry Areas (ICARDA), Rabat 10106, Morocco
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Academic Editor: Marcello Iriti
Received: 28 April 2017 / Revised: 23 May 2017 / Accepted: 25 May 2017 / Published: 6 June 2017
(This article belongs to the Special Issue Pulses)
View Full-Text   |   Download PDF [12429 KB, uploaded 6 June 2017]   |  

Abstract

One of the traits studied by Mendel in pea (Pisum sativum L.) was the wrinkled-seeded phenotype, and the molecular basis for a mutation underlying this phenotype was discovered in the 1990s. Although the starch-branching enzyme gene mutation identified at the genetic locus r is most likely to be that in seeds available to Mendel in the mid-1800s, it has remained an open question as to whether or not additional natural mutations in this gene exist within Pisum germplasm collections. Here, we explore this question and show that all but two wrinkled-seeded variants in one such collection correspond to either the mutant allele described previously for the r locus or a mutation at a second genetic locus, rb, affecting the gene encoding the large subunit of Adenosine diphosphoglucose (ADP-glucose) pyrophosphorylase; the molecular basis for the rb mutation is described here. The genetic basis for the phenotype of one (JI 2110) of the two lines which are neither r nor rb has been studied in crosses with a round-seeded variant (JI 281); for which extensive genetic marker data were expected. In marked contrast to the trait studied by Mendel and the rb phenotype; the data suggest that the wrinkled-seeded phenotype in JI 2110 is maternally determined, controlled by two genetic loci, and the extent to which it is manifested is very sensitive to the environment. Metabolite analysis of the cotyledons of JI 2110 revealed a profile for sucrose and sucrose-derived compounds that was more similar to that of wild-type round-seeded, than that of wrinkled-seeded r, pea lines. However, the metabolite profile of the seed coat (testa) of JI 2110 was distinct from that of other round-seeded genotypes tested which, together with analysis of recombinant inbred progeny lines, suggests an explanation for the seed phenotype. View Full-Text
Keywords: genetic markers; myoinositol; pea germplasm; r and rb mutations; seed phenotype; seed coat (testa) metabolites; wrinkled seeds genetic markers; myoinositol; pea germplasm; r and rb mutations; seed phenotype; seed coat (testa) metabolites; wrinkled seeds
Figures

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Supplementary material

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Rayner, T.; Moreau, C.; Ambrose, M.; Isaac, P.G.; Ellis, N.; Domoney, C. Genetic Variation Controlling Wrinkled Seed Phenotypes in Pisum: How Lucky Was Mendel? Int. J. Mol. Sci. 2017, 18, 1205.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top