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Int. J. Mol. Sci. 2016, 17(12), 2013; doi:10.3390/ijms17122013

Cytokinins and Expression of SWEET, SUT, CWINV and AAP Genes Increase as Pea Seeds Germinate

1
School of Biological Sciences, University of Canterbury, Private Bag 4800, Christchurch 8140, New Zealand
2
Laboratory of Growth Regulators, Centre of the Region Haná for Biotechnological and Agricultural Research, Institute of Experimental Botany CAS & Faculty of Science of Palacký University, Šlechtitelů 27, 783 71 Olomouc, Czech Republic
3
School of Life Sciences, Yantai University, Yantai 264005, China
*
Author to whom correspondence should be addressed.
Academic Editor: Marcello Iriti
Received: 26 October 2016 / Revised: 24 November 2016 / Accepted: 25 November 2016 / Published: 1 December 2016
(This article belongs to the Special Issue Pulses)
View Full-Text   |   Download PDF [749 KB, uploaded 1 December 2016]   |  

Abstract

Transporter genes and cytokinins are key targets for crop improvement. These genes are active during the development of the seed and its establishment as a strong sink. However, during germination, the seed transitions to being a source for the developing root and shoot. To determine if the sucrose transporter (SUT), amino acid permease (AAP), Sugar Will Eventually be Exported Transporter (SWEET), cell wall invertase (CWINV), cytokinin biosynthesis (IPT), activation (LOG) and degradation (CKX) gene family members are involved in both the sink and source activities of seeds, we used RT-qPCR to determine the expression of multiple gene family members, and LC-MS/MS to ascertain endogenous cytokinin levels in germinating Pisum sativum L. We show that genes that are actively expressed when the seed is a strong sink during its development, are also expressed when the seed is in the reverse role of being an active source during germination and early seedling growth. Cytokinins were detected in the imbibing seeds and were actively biosynthesised during germination. We conclude that, when the above gene family members are targeted for seed yield improvement, a downstream effect on subsequent seed germination or seedling vigour must be taken into consideration. View Full-Text
Keywords: cytokinin; germination; Pisum sativum cytokinin; germination; Pisum sativum
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MDPI and ACS Style

Jameson, P.E.; Dhandapani, P.; Novak, O.; Song, J. Cytokinins and Expression of SWEET, SUT, CWINV and AAP Genes Increase as Pea Seeds Germinate. Int. J. Mol. Sci. 2016, 17, 2013.

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