Special Issue "Aptasensors"
Deadline for manuscript submissions: closed (31 October 2013)
Dr. Beate Strehlitz
Department Environmental and Biotechnology Centre, Helmholtz Centre for Environmental Research - UFZ, Permoserstr. 15, 04318 Leipzig, Germany
Phone: +49 341 235 1764
Fax: +49 341 235 1764
Interests: biosensor development and application; aptamer selection and characterization; aptamer based sensors and assays
Aptamers are a very attractive class of biologic receptor molecules being in great demand in the development of new biosensors, so-called aptasensors. Aptamers are short single-stranded DNA or RNA oligonucleotides able to bind with high affinity and specificity to their target. They are usually generated by an in vitro selection and amplification technology (SELEX). From a large oligonucleotide library with big sequence diversity and structural complexity only those oligonucleotides are selected and enriched during several selection rounds which can bind very tightly to the specific molecular target. Aptamer development and application increased in the last decades. Aptamers are favourably used in biosensors as sensitive and selective bio-receptors coupled with a variety of transducer principles such as optical, mass-sensitive and electrochemical detection. The analytes cover a wide range from small molecules, like nucleotides, cofactors, amino acids, organic molecules over peptides, polysaccharides and proteins to complex structures like whole cells, viruses and single cell organisms. Aptasensors offer great potential to measure substances in clinical diagnostics, environmental analytics, food and biotechnology industries, process engineering and others. The Special Issue will publish those full research, review and high rated manuscripts addressing the development and application of aptamer based biosensors.
Dr. Beate Strehlitz
Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. Papers will be published continuously (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.
Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are refereed through a peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Sensors is an international peer-reviewed Open Access monthly journal published by MDPI.
- affinity sensor
- mass sensitive
- label- and label-free detection
Article: Development of a Multiplex Sandwich Aptamer Microarray for the Detection of VEGF165 and Thrombin
Sensors 2013, 13(10), 13425-13438; doi:10.3390/s131013425
Received: 30 August 2013; in revised form: 20 September 2013 / Accepted: 29 September 2013 / Published: 3 October 2013| Download PDF Full-text (517 KB) | Download XML Full-text | Supplementary Files
Sensors 2013, 13(10), 13624-13637; doi:10.3390/s131013624
Received: 7 August 2013; in revised form: 24 September 2013 / Accepted: 27 September 2013 / Published: 10 October 2013| Download PDF Full-text (449 KB) | Download XML Full-text
Sensors 2013, 13(11), 14543-14557; doi:10.3390/s131114543
Received: 29 August 2013; in revised form: 17 October 2013 / Accepted: 18 October 2013 / Published: 25 October 2013| Download PDF Full-text (1128 KB)
Review: Recent Advances and Achievements in Nanomaterial-Based, and Structure Switchable Aptasensing Platforms for Ochratoxin A Detection
Sensors 2013, 13(11), 15187-15208; doi:10.3390/s131115187
Received: 25 September 2013; in revised form: 28 October 2013 / Accepted: 4 November 2013 / Published: 6 November 2013| Download PDF Full-text (396 KB)
Article: Impedimetric Aptasensor for Ochratoxin A Determination Based on Au Nanoparticles Stabilized with Hyper-Branched Polymer
Sensors 2013, 13(12), 16129-16145; doi:10.3390/s131216129
Received: 22 October 2013; in revised form: 14 November 2013 / Accepted: 18 November 2013 / Published: 26 November 2013| Download PDF Full-text (1430 KB)
Sensors 2013, 13(12), 16292-16311; doi:10.3390/s131216292
Received: 31 October 2013; in revised form: 20 November 2013 / Accepted: 21 November 2013 / Published: 28 November 2013| Download PDF Full-text (598 KB)
The below list represents only planned manuscripts. Some of these manuscripts have not been received by the Editorial Office yet. Papers submitted to MDPI journals are subject to peer-review.
Type of Paper: Article
Title: Towards a Solid-Phase Assay for Thrombin Detection Using Upconversion Nanoparticles as Donors and Quantum Dots as Acceptors in Luminescence Resonance Energy Transfer
Authors: Samer Doughan and Ulrich J. Krull
Affiliation: University of Toronto Mississauga, 3359 Mississauga Road North, Mississauga, ON L5L 1C6, Canada; E-Mail: firstname.lastname@example.org
Abstract: A solid-phase assay will be presented for the selective detection of thrombin using immobilized upconversion nanoparticles (UCNPs) as donors in luminescence resonance energy transfer (LRET). A sandwich complex will be described that consists of two thrombin-binding aptamers (TBA 1 and TBA 2). The surface of optical fibers will be modified with biotin to immobilize green-emitting (542 nm) streptavidin coated UCNPs, which then will be decorated with TBA 1. Thrombin will be sandwiched between immobilized TBA 1 and TBA 2, and this will then be functionalized with red-emitting (655 nm) streptavidin coated QDs as acceptors. The spectroscopic properties of the nanoparticles, the assessment of solid phase assembly, and the figures of merit (selectivity, sensitivity, speed and the extent of regeneration) of the evanescent wave sensor constructs will be reported.
Type of Paper: Article
Title: Aptamers as Theranostic Agents: Modifications, Serum Stability and Functionalisation
Authors: Sarah Shigdar 1, Joanna Macdonald 1, Tao Wang 1, Dongxi Xiang 1, Hadi Al.Shamaileh 1, Ming Q Wei 2, Jia Lin 3, Yimin Zhu 4 and Wei Duan 1
Affiliations: 1 School of Medicine, Faculty of Health, Deakin University, Waurn Ponds, Victoria, 3216, Australia; E-Mail: email@example.com
2 School of Medical Science and Griffith Health Institute, Griffith University, Gold Coast Campus, Southport, Australia
3 Department of Biochemistry and Molecular Biology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu, China
4 Suzhou Institute of Nano-Tech & Nano-bionics, Chinese Academy of Science, Suzhou 215123, China
Abstract: Aptamers, and the selection process used to generate them, were first described more than twenty years ago. Since then, there have been numerous changes to the selection procedure (Systematic Evolution of Ligands by Exponential Enrichment (SELEX)), the production of DNA enzymes thus allowing the integration of modified bases during the selection process, and the generation of modified bases which can be added into the aptamer structure following selection and truncation. This review discusses the use of modified bases as a means of enhancing serum stability and producing effective therapeutic tools, as well as functionalising these nucleic acids to be used as diagnostic agents.
Type of Paper: Article
Title: Aptamer Based Analysis: A promising Alternative for Food Safety Control
Authors: Sonia Amaya-González, Noemí de-los-Santos-Álvarez, Arturo J. Miranda-Ordieres and Maria Jesús Lobo-Castañón *
Affiliation: Departamento de Química-Física y Analítica. Universidad de Oviedo, Julián Clavería, 8, 33006 Oviedo, Spain; * E-Mail: firstname.lastname@example.org
Abstract: Ensuring food safety is nowadays a top priority of authorities and professional players in the food supply chain. One of the key challenges to determine the safety of food and guarantee a high level of consumer protection is the availability of fast, sensitive and reliable analytical methods to identify specific hazards associated to food before they become a health problem. The limitation of existing methods has encouraged the development of new technologies, among them biosensors. Success in biosensor design depends largely on the development of novel receptors with enhanced affinity to the target, while being stable and economical. Aptamers fulfil these characteristics and have therefore surfaced as promising alternatives to natural receptors. This Feature describes analytical strategies developed so far using aptamers for the control of pathogens, allergens, adulterants, toxins and other forbidden contaminants to ensure food safety. The main progresses to date are presented, highlighting potential prospects for the future.
Type of Paper: Article
Title: Aptamer-Target Binding Assays and Their Relevance to Aptasensor Design
Authors: Thao T. Le and Anthony E. G. Cass
Affiliation: Dept. of Chemistry, Imperial College London, London SW7 2AZ, UK; E-Mail: email@example.com
Abstract: Determination of the affinity of an aptamer for its target molecule is a key step in designing aptasensors. The affinity (typically expressed as a dissociation constant) will define the concentration range over which the aptasensor works. Unlike antibodies, nucleic acid aptamers are selected under a wide range of solution conditions and the sensitivity of the affinity to these different conditions can often be much greater than with antibodies. For these reasons it is important that the methods to measure aptamer-target binding affinities should reflect as closely as possible those that pertain during the use to which the sensor is put. Many different binding assays have been described in the literature, some adapted from immunoassays and some from investigations of natural nucleic acid-ligand binding processes. We review here theses different methods, highlight their strengths and limitations and how these relate to aptasensor performance.
Type of Paper: Article
Title: Recent Advances and Achievements in Nanomaterial-Based, and Structure Switchable Aptasensing Platforms for Ochratoxin A Detection
Authors: Akhtar Hayat 1,2, Cheng Yang 1, Amina Rhouati 1 and Jean Louis Marty 1
Affiliations : 1 IMAGES, Universite de Perpignan, 52 Avenue Paul Alduy, 66860 Perpignan Cedex, France; E-Mail: firstname.lastname@example.org
2 Department of Chemistry and Biomolecular science, Clarkson University, Potsdam, NY 13699-5810, USA
Abstract: Aptamer-based bioreceptors that can easily adopt their surroundings have captured the attention of scientist from a wide spectrum of domains in designing highly sensitive, selective and structure switchable sensing assays. Through elaborate design and chemical functionalization, numerous aptamer-based assays have been developed that can switch their conformation upon incubation with target analyte, resulting in an enhanced output signal. To further lower the detection limits at picomolar level, nanomaterials have attained great interest in the design of aptamer based sensing platforms. Associated to their unique properties, nanomaterials offer great promise for numerous aptasensing applications. This review will discuss current research activities in the aptasensing with typical example of detection of ochratoxin A (OTA). OTA, a secondary fungal metabolite, contaminates a variety of food commodities, and has several toxicological effects such as nephrotoxic, hepatotoxic, neurotoxic, teratogenic and immunotoxic. The review will introduce advances made in the methods of integrating nanomaterials in aptasensing, and will discuss current conformational switchable designed strategies in the aptasensor fabrication methodologies.
Keywords: aptasensor; nanomaterials; aptamer-conjugated nanoparticles; structure switchable; ochratoxin A
Last update: 12 August 2013