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Biostable ssDNA Aptamers Specific for Hodgkin Lymphoma
Department of Pathology and Genomic Medicine, Houston Methodist Hospital, and Cancer Pathology Research Laboratory, Houston Methodist Research Institute, Houston, TX 77030, USA
* Author to whom correspondence should be addressed.
Received: 29 August 2013; in revised form: 17 October 2013 / Accepted: 18 October 2013 / Published: 25 October 2013
(This article belongs to the Special Issue Aptasensors
Abstract: As a “chemical antibody”, oligonucleotide aptamers can specifically bind to their target molecules. However, clinical potential of aptamers in disease diagnosis is not yet fully explored. Using a tumor cell-based selection protocol, we developed single-stranded DNA aptamers for Hodgkin lymphoma (HL) tumor cells. The aptamers specifically bound to HL cells with a high affinity, reaching maximal cell binding at 10 nM final concentration. Importantly, the aptamers were able to selectively detect HL cells and did not react to other tumor or blood cells in mixed samples, indicating that the aptamers can be used as a specific probe for in vitro analysis of HL cells. Moreover, due to the inherent properties of DNA, the aptamers were stable in human serum, suggesting potential for in vivo detection of HL tumor cells.
Keywords: aptamer; biostability; Hodgkin lymphoma; single-stranded DNA (ssDNA); tumor cell detection
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MDPI and ACS Style
Parekh, P.; Kamble, S.; Zhao, N.; Zeng, Z.; Wen, J.; Yuan, B.; Zu, Y. Biostable ssDNA Aptamers Specific for Hodgkin Lymphoma. Sensors 2013, 13, 14543-14557.
Parekh P, Kamble S, Zhao N, Zeng Z, Wen J, Yuan B, Zu Y. Biostable ssDNA Aptamers Specific for Hodgkin Lymphoma. Sensors. 2013; 13(11):14543-14557.
Parekh, Parag; Kamble, Sanchit; Zhao, Nianxi; Zeng, Zihua; Wen, Jianguo; Yuan, Bin; Zu, Youli. 2013. "Biostable ssDNA Aptamers Specific for Hodgkin Lymphoma." Sensors 13, no. 11: 14543-14557.