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DNA-Aptamers Binding Aminoglycoside Antibiotics
Helmholtz Centre for Environmental Research (UFZ), Permoserstraße 15, Leipzig 04318, Germany
* Author to whom correspondence should be addressed.
Received: 29 October 2013; in revised form: 20 January 2014 / Accepted: 30 January 2014 / Published: 21 February 2014
(This article belongs to the Special Issue Aptasensors
Abstract: Aptamers are short, single stranded DNA or RNA oligonucleotides that are able to bind specifically and with high affinity to their non-nucleic acid target molecules. This binding reaction enables their application as biorecognition elements in biosensors and assays. As antibiotic residues pose a problem contributing to the emergence of antibiotic-resistant pathogens and thereby reducing the effectiveness of the drug to fight human infections, we selected aptamers targeted against the aminoglycoside antibiotic kanamycin A with the aim of constructing a robust and functional assay that can be used for water analysis. With this work we show that aptamers that were derived from a Capture-SELEX procedure targeting against kanamycin A also display binding to related aminoglycoside antibiotics. The binding patterns differ among all tested aptamers so that there are highly substance specific aptamers and more group specific aptamers binding to a different variety of aminoglycoside antibiotics. Also the region of the aminoglycoside antibiotics responsible for aptamer binding can be estimated. Affinities of the different aptamers for their target substance, kanamycin A, are measured with different approaches and are in the micromolar range. Finally, the proof of principle of an assay for detection of kanamycin A in a real water sample is given.
Keywords: aptamer; Capture-SELEX; aminoglycoside antibiotic; kanamycin
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MDPI and ACS Style
Nikolaus, N.; Strehlitz, B. DNA-Aptamers Binding Aminoglycoside Antibiotics. Sensors 2014, 14, 3737-3755.
Nikolaus N, Strehlitz B. DNA-Aptamers Binding Aminoglycoside Antibiotics. Sensors. 2014; 14(2):3737-3755.
Nikolaus, Nadia; Strehlitz, Beate. 2014. "DNA-Aptamers Binding Aminoglycoside Antibiotics." Sensors 14, no. 2: 3737-3755.