Next Article in Journal
Continuous Monitoring of Turning in Patients with Movement Disability
Next Article in Special Issue
Smart Materials Based on DNA Aptamers: Taking Aptasensing to the Next Level
Previous Article in Journal
Bio-Mimic Optimization Strategies in Wireless Sensor Networks: A Survey
Previous Article in Special Issue
Aptamer-Based Analysis: A Promising Alternative for Food Safety Control
Article Menu

Export Article

Open AccessArticle
Sensors 2014, 14(1), 346-355; doi:10.3390/s140100346

Development of an Antigen-DNAzyme Based Probe for a Direct Antibody-Antigen Assay Using the Intrinsic DNAzyme Activity of a Daunomycin Aptamer

Institute for Research in Molecular Medicine, Universiti Sains Malaysia, Minden 11800, Penang, Malaysia
Department of Molecular Biotechnology and Functional Genomics, Technical University of Applied Sciences Wildau, Bahnhofstr. 1, Wildau 15745, Germany
Author to whom correspondence should be addressed.
Received: 31 October 2013 / Revised: 9 December 2013 / Accepted: 13 December 2013 / Published: 27 December 2013
(This article belongs to the Special Issue Aptasensors)
View Full-Text   |   Download PDF [407 KB, uploaded 21 June 2014]   |  


G-Quadruplex (G-4) structures are formed when G-rich DNA sequences fold into intra- or intermolecular four-stranded structures in the presence of metal ions. G-4-hemin complexes are often effective peroxidase-mimicking DNAzymes that are applied in many detection systems. This work reports the application of a G-rich daunomycin-specific aptamer for the development of an antibody-antigen detection assay. We investigated the ability of the daunomycin aptamer to efficiently catalyze the hemin-dependent peroxidase activity independent of daunomycin. A reporter probe consisting of biotinylated antigen and daunomycin aptamer coupled to streptavidin gold nanoparticles was successfully used to generate a colorimetric readout. In conclusion, the daunomycin aptamer can function as a robust alternative DNAzyme for the development of colorimetric assays.
Keywords: G-quadruplex; DNAzyme; gold nanoparticles; antibody G-quadruplex; DNAzyme; gold nanoparticles; antibody
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

Supplementary material

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Omar, N.; Loh, Q.; Tye, G.J.; Choong, Y.S.; Noordin, R.; Glökler, J.; Lim, T.S. Development of an Antigen-DNAzyme Based Probe for a Direct Antibody-Antigen Assay Using the Intrinsic DNAzyme Activity of a Daunomycin Aptamer. Sensors 2014, 14, 346-355.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics



[Return to top]
Sensors EISSN 1424-8220 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top