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Sensors 2014, 14(1), 346-355; doi:10.3390/s140100346
Article

Development of an Antigen-DNAzyme Based Probe for a Direct Antibody-Antigen Assay Using the Intrinsic DNAzyme Activity of a Daunomycin Aptamer

1
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1
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1
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1
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1
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2
 and
1,*
1 Institute for Research in Molecular Medicine, Universiti Sains Malaysia, Minden 11800, Penang, Malaysia 2 Department of Molecular Biotechnology and Functional Genomics, Technical University of Applied Sciences Wildau, Bahnhofstr. 1, Wildau 15745, Germany
* Author to whom correspondence should be addressed.
Received: 31 October 2013 / Revised: 9 December 2013 / Accepted: 13 December 2013 / Published: 27 December 2013
(This article belongs to the Special Issue Aptasensors)
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Abstract

G-Quadruplex (G-4) structures are formed when G-rich DNA sequences fold into intra- or intermolecular four-stranded structures in the presence of metal ions. G-4-hemin complexes are often effective peroxidase-mimicking DNAzymes that are applied in many detection systems. This work reports the application of a G-rich daunomycin-specific aptamer for the development of an antibody-antigen detection assay. We investigated the ability of the daunomycin aptamer to efficiently catalyze the hemin-dependent peroxidase activity independent of daunomycin. A reporter probe consisting of biotinylated antigen and daunomycin aptamer coupled to streptavidin gold nanoparticles was successfully used to generate a colorimetric readout. In conclusion, the daunomycin aptamer can function as a robust alternative DNAzyme for the development of colorimetric assays.
Keywords: G-quadruplex; DNAzyme; gold nanoparticles; antibody G-quadruplex; DNAzyme; gold nanoparticles; antibody
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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Omar, N.; Loh, Q.; Tye, G.J.; Choong, Y.S.; Noordin, R.; Glökler, J.; Lim, T.S. Development of an Antigen-DNAzyme Based Probe for a Direct Antibody-Antigen Assay Using the Intrinsic DNAzyme Activity of a Daunomycin Aptamer. Sensors 2014, 14, 346-355.

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