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Sensors 2014, 14(1), 346-355; doi:10.3390/s140100346
Article

Development of an Antigen-DNAzyme Based Probe for a Direct Antibody-Antigen Assay Using the Intrinsic DNAzyme Activity of a Daunomycin Aptamer

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Received: 31 October 2013 / Revised: 9 December 2013 / Accepted: 13 December 2013 / Published: 27 December 2013
(This article belongs to the Special Issue Aptasensors)
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Abstract

G-Quadruplex (G-4) structures are formed when G-rich DNA sequences fold into intra- or intermolecular four-stranded structures in the presence of metal ions. G-4-hemin complexes are often effective peroxidase-mimicking DNAzymes that are applied in many detection systems. This work reports the application of a G-rich daunomycin-specific aptamer for the development of an antibody-antigen detection assay. We investigated the ability of the daunomycin aptamer to efficiently catalyze the hemin-dependent peroxidase activity independent of daunomycin. A reporter probe consisting of biotinylated antigen and daunomycin aptamer coupled to streptavidin gold nanoparticles was successfully used to generate a colorimetric readout. In conclusion, the daunomycin aptamer can function as a robust alternative DNAzyme for the development of colorimetric assays.
Keywords: G-quadruplex; DNAzyme; gold nanoparticles; antibody G-quadruplex; DNAzyme; gold nanoparticles; antibody
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Omar, N.; Loh, Q.; Tye, G.J.; Choong, Y.S.; Noordin, R.; Glökler, J.; Lim, T.S. Development of an Antigen-DNAzyme Based Probe for a Direct Antibody-Antigen Assay Using the Intrinsic DNAzyme Activity of a Daunomycin Aptamer. Sensors 2014, 14, 346-355.

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