Special Issue "Cell and Tissue Metabolomics"
A special issue of Metabolites (ISSN 2218-1989).
Deadline for manuscript submissions: 30 June 2014
Dr. Justin J.J. van der Hooft
Plant Products and Human Nutrition group, North Lab, Joseph Black Building, University of Glasgow, Glasgow G12 8QQ, UK
Interests: mass spectrometry; mass spectrometry fragmentation; nuclear magnetic resonance spectroscopy; metabolite identification; structural elucidation; metabolite annotation; automated metabolite annotation; polyphenols; bioavailability; metabolomics; urine
The last two decades we have witnessed an exciting development of metabolomics techniques and approaches. Without disregarding the other analytical tools, the two main analytical pillars are currently mass spectrometry (MS) and nuclear magnetic resonance spectroscopy (NMR). The total extracts of numerous plants, yeast, and human bio fluids were comprehensively characterised. With the equipment getting increasingly sensitive, we now see the first examples of cell and tissue metabolomes being, at least partially, resolved, with cancer cells and tissues being in the centre of attention.
Again the main challenges to be expected are: i) can we fully elucidate all detected metabolites, and ii) can we develop protocols for robust extraction and detection of the small metabolites? There are several interesting questions to be addressed: a) how can we learn from previous metabolomics studies, b) how can we organize the tremendous amount of data and metadata on metabolites in the most efficient way, and c) how can software tools help us solving the metabolomics challenges?
Herewith I invite you to contribute to this special issue of Metabolites: original research, a reviewing of the initial attempts, and ideas on how to tackle the cell and tissue metabolomics challenges in the nearby future are all welcome, from both the plant and human metabolomics field.
Dr. Justin J.J. van der Hooft
Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. Papers will be published continuously (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.
Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are refereed through a peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Metabolites is an international peer-reviewed Open Access quarterly journal published by MDPI.
Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 500 CHF (Swiss Francs). English correction and/or formatting fees of 250 CHF (Swiss Francs) will be charged in certain cases for those articles accepted for publication that require extensive additional formatting and/or English corrections.
- cell metabolomics
- tissue metabolomics
- metabolite annotation
- metabolite identification
- mass spectrometry
- spectral databases
- compound databases
- sample preparation techniques
- metabolic networks
Article: Comparative Analysis of Biological Sphingolipids with Glycerophospholipids and Diacylglycerol by LC-MS/MS
Metabolites 2014, 4(1), 98-114; doi:10.3390/metabo4010098
Received: 4 December 2013; in revised form: 8 January 2014 / Accepted: 20 January 2014 / Published: 27 January 2014| Download PDF Full-text (978 KB) | Download XML Full-text | Supplementary Files
The below list represents only planned manuscripts. Some of these manuscripts have not been received by the Editorial Office yet. Papers submitted to MDPI journals are subject to peer-review.
Title: Unraveling Signaling and Biochemical Pathways Induced by Mitochondrial Dysfunctions by Using Metabolomic Approaches
Authors: S. Demine1, R. Nagabushana1, M. Raes1, P. Renard1 and T. Arnould1
Affiliation: 1 Unité de Recherche en Biologie Cellulaire (URBC), NAmur Research Institute for Life Sciences (NARILIS), University of Namur (UNamur), Belgium
Abstract: Mitochondrial dysfunction(s) (MDs) can be defined as an alteration of the mitochondria including mitochondrial uncoupling, mitochondrial depolarization, inhibition of mitochondrial respiratory chain, mitochondrial network fragmentation, mitochondrial or nuclear DNA mutations and mitochondrial accumulation of protein aggregates. These MDs are known to be induced by several pathological states/diseases including cancer, obesity, muscular and neurological troubles. On the other hand, these conditions can also induce several mitochondrial dysfunctions by themselves. Induction of MDs can alter secretion of several metabolites (i.e. pro-inflammatory cytokines), reactive oxygen species (ROS) production and modify several cell signaling pathways (cytosolic calcium concentration, AMPK, PI3K, Akt, PKC, MEK pathways) in order to attempt to resolve the mitochondrial dysfunction or to ultimately trigger cell death. Along with ROS, many metabolites, such as fatty acids and derived compounds, could be also secreted into the blood stream by cells suffering from mitochondrial dysfunctions. In this review, we propose to summarize how some metabolites are able to induce or can be modified by a mitochondrial dysfunction, along with the signaling pathways and transcription factors involved in this process, with a strong emphasis on MU. For this purpose, we will summarize how it is possible to identify consequences or causes of a mitochondrial dysfunction by using complementary transcriptomics, proteomics (2D gels, liquid chromatography, gas chromatography) and metabolomics (liquid chromatography associated with mass spectrometry analysis, NMR spectrometry, PET scan, nanofluidic analysis of isolated mitochondria) approaches.
Last update: 6 February 2014