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Metabolites 2014, 4(1), 98-114; doi:10.3390/metabo4010098

Comparative Analysis of Biological Sphingolipids with Glycerophospholipids and Diacylglycerol by LC-MS/MS

1
Department of Hematology/Immunity, Kanazawa Medical University, Uchinada-machi, Kahoku-gun, Ishikawa 920-0293, Japan
2
Medical Research Institute, Kanazawa Medical University, Uchinada-machi, Kahoku-gun, Ishikawa 920-0293, Japan
3
Division of Molecular Pathology, Faculty of Medicine, Tottori University, Nishi-cho, Yonago 683-8503, Japan
*
Author to whom correspondence should be addressed.
Received: 4 December 2013 / Revised: 8 January 2014 / Accepted: 20 January 2014 / Published: 27 January 2014
(This article belongs to the Special Issue Cell and Tissue Metabolomics)
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Abstract

Liquid chromatography-electrospray ionization mass spectrometry (LC-MS) is an effective and popular technique used in lipid metabolomic studies. Although many LC-MS methods enabling the determination of sphingolipid molecular species have been reported, they do not cover a broad range of sphingolipid metabolites with expanding glycerophospholipids (GPLs) and diacylglycerol (DAG). In this study, we developed an approach for the comprehensive analysis of sphingolipids, GPLs and DAG molecular species in a biological sample, without alkaline hydrolysis. After validating the reliability of this approach, we analyzed tissue lipids of sphingomyelin synthase 2-knockout mice and found that changes in sphingolipid metabolism in the liver affect the level of docosahexaenoic acid-containing GPLs. Our method analyzes GPLs and DAG, as well as sphingolipids within biological samples and, thus, will facilitate more comprehensive studies of sphingolipid metabolism in pathology and diagnostics. View Full-Text
Keywords: sphingolipid; glycerophospholipid; diacylglycerol; sphingomyelin; ceramide; sphingosine-1-phosphate; phosphatidylcholine; liquid chromatography; mass spectrometry; lipidomics sphingolipid; glycerophospholipid; diacylglycerol; sphingomyelin; ceramide; sphingosine-1-phosphate; phosphatidylcholine; liquid chromatography; mass spectrometry; lipidomics
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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MDPI and ACS Style

Ogiso, H.; Taniguchi, M.; Araya, S.; Aoki, S.; Wardhani, L.O.; Yamashita, Y.; Ueda, Y.; Okazaki, T. Comparative Analysis of Biological Sphingolipids with Glycerophospholipids and Diacylglycerol by LC-MS/MS. Metabolites 2014, 4, 98-114.

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