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Metabolites 2014, 4(1), 98-114; doi:10.3390/metabo4010098
Article

Comparative Analysis of Biological Sphingolipids with Glycerophospholipids and Diacylglycerol by LC-MS/MS

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Received: 4 December 2013; in revised form: 8 January 2014 / Accepted: 20 January 2014 / Published: 27 January 2014
(This article belongs to the Special Issue Cell and Tissue Metabolomics)
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Abstract: Liquid chromatography-electrospray ionization mass spectrometry (LC-MS) is an effective and popular technique used in lipid metabolomic studies. Although many LC-MS methods enabling the determination of sphingolipid molecular species have been reported, they do not cover a broad range of sphingolipid metabolites with expanding glycerophospholipids (GPLs) and diacylglycerol (DAG). In this study, we developed an approach for the comprehensive analysis of sphingolipids, GPLs and DAG molecular species in a biological sample, without alkaline hydrolysis. After validating the reliability of this approach, we analyzed tissue lipids of sphingomyelin synthase 2-knockout mice and found that changes in sphingolipid metabolism in the liver affect the level of docosahexaenoic acid-containing GPLs. Our method analyzes GPLs and DAG, as well as sphingolipids within biological samples and, thus, will facilitate more comprehensive studies of sphingolipid metabolism in pathology and diagnostics.
Keywords: sphingolipid; glycerophospholipid; diacylglycerol; sphingomyelin; ceramide; sphingosine-1-phosphate; phosphatidylcholine; liquid chromatography; mass spectrometry; lipidomics sphingolipid; glycerophospholipid; diacylglycerol; sphingomyelin; ceramide; sphingosine-1-phosphate; phosphatidylcholine; liquid chromatography; mass spectrometry; lipidomics
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Ogiso, H.; Taniguchi, M.; Araya, S.; Aoki, S.; Wardhani, L.O.; Yamashita, Y.; Ueda, Y.; Okazaki, T. Comparative Analysis of Biological Sphingolipids with Glycerophospholipids and Diacylglycerol by LC-MS/MS. Metabolites 2014, 4, 98-114.

AMA Style

Ogiso H, Taniguchi M, Araya S, Aoki S, Wardhani LO, Yamashita Y, Ueda Y, Okazaki T. Comparative Analysis of Biological Sphingolipids with Glycerophospholipids and Diacylglycerol by LC-MS/MS. Metabolites. 2014; 4(1):98-114.

Chicago/Turabian Style

Ogiso, Hideo; Taniguchi, Makoto; Araya, Shinichi; Aoki, Shinya; Wardhani, Lusi O.; Yamashita, Yuka; Ueda, Yoshibumi; Okazaki, Toshiro. 2014. "Comparative Analysis of Biological Sphingolipids with Glycerophospholipids and Diacylglycerol by LC-MS/MS." Metabolites 4, no. 1: 98-114.


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