Special Issue "Isotope Guided Metabolomics and Flux Analysis"
A special issue of Metabolites (ISSN 2218-1989).
Deadline for manuscript submissions: closed (31 October 2017)
Dr. Wolfgang Eisenreich
Labeling experiments using stable isotopes are ma jor tools in the analysis of biosynthetic and metabolic processes. Moreover, stable isotope labeled metabolites are widely used as internal references to quantify compounds by isotope dilution analysis. Both fields are benefitted by the dramatic progress in mass spectrometry and NMR instrumentation and processing. The Special Issue of Metabolites, "Isotope Guided Metabolomics and Flux Analysis", will be focused on cutting-edge technologies using stable isotopes for metabolic analysis both from a fundamental as well as an applied point of view. The topics that shall be covered by this Special Issue include recent developments and examples in isotope dilution analysis, as well as in isotope guided metabolic pathway and flux analysis. A special focus shall be given to 13C-labeling methods on a global and single cell level, recent developments and applications in metabolic flux analysis, and applications in the analysis of food and pharmaceutical products. Manuscripts dealing with other challenging issues in the field of isotope guided metabolomics and flux analysis are also highly welcome.
Dr. Wolfgang Eisenreich
Manuscript Submission Information
Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All papers will be peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.
Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Metabolites is an international peer-reviewed open access quarterly journal published by MDPI.
Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 850 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.
The below list represents only planned manuscripts. Some of these manuscripts have not been received by the Editorial Office yet. Papers submitted to MDPI journals are subject to peer-review.
Tentative title: Isotope-assisted evaluation of artifacts created by the use of methanol as extraction solvent in metabolomics
Author: Claudia Sauerschnig, Maria Doppler, Christoph Bueschl, Alexandra Maria Simader, Rainer Schuhmacher
Affiliation: Center for Analytical Chemistry, Department of Agrobiotechnology (IFA-Tulln), University of Natural Resources and Life
Sciences, Vienna (BOKU), Tulln an der Donau 3430, Austria
Tentative Abstract:In many metabolomics studies mixtures of methanol and water with or without the addition of formic acid are used for sample extraction. Especially in untargeted approaches, where many metabolites are unknown at the time of analysis, solvent-derived artifacts might erroneously be taken as true biochemical constituents.
In the present study wheat leaves were extracted with mixtures of acidified (0.1% HCOOH) native and labelled CD3OH and water. The resulting extracts were analysed immediately and after storage at 10 °C, -20 °C or at -80 C by the use of an HPLC-ESI-Q-Exactive HF Orbitrap instrument with polarity switching in the full scan mode. Isotope-assisted data processing was performed with the MetExtract II software and Thermo Xcalibur. Our study revealed that numerous methylated metabolites had been formed during sample extraction. Moreover, storage of samples at 10 °C for several days, as can typically be the case during longer measurement sequences, led to an increase in both intensity and number of methylated artifacts. The paper describes the isotope-assisted workflow for artifact detection and the characterisation of the methanol-derived reaction products.