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Metabolites 2017, 7(4), 52; https://doi.org/10.3390/metabo7040052

Bacterial Substrate Transformation Tracked by Stable-Isotope-Guided NMR Metabolomics: Application in a Natural Aquatic Microbial Community

1
RIKEN Center for Sustainable Resource Science, 1-7-22 Suehiro, Tsurumi, Yokohama, Kanagawa 230-0045, Japan
2
Graduate School of Medical Life Science, Yokohama City University, 1-7-29 Suehiro, Tsurumi, Yokohama, Kanagawa 230-0045, Japan
3
Graduate School of Bioagricultural Sciences and School of Agricultural Sciences, Nagoya University, 1 Furo, Chikusa, Nagoya 464-8601, Japan
*
Authors to whom correspondence should be addressed.
Received: 12 September 2017 / Revised: 8 October 2017 / Accepted: 16 October 2017 / Published: 19 October 2017
(This article belongs to the Special Issue Isotope Guided Metabolomics and Flux Analysis)
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Abstract

The transformation of organic substrates by heterotrophic bacteria in aquatic environments constitutes one of the key processes in global material cycles. The development of procedures that would enable us to track the wide range of organic compounds transformed by aquatic bacteria would greatly improve our understanding of material cycles. In this study, we examined the applicability of nuclear magnetic resonance spectroscopy coupled with stable-isotope labeling to the investigation of metabolite transformation in a natural aquatic bacterial community. The addition of a model substrate (13C6–glucose) to a coastal seawater sample and subsequent incubation resulted in the detection of >200 peaks and the assignment of 22 metabolites from various chemical classes, including amino acids, dipeptides, organic acids, nucleosides, nucleobases, and amino alcohols, which had been identified as transformed from the 13C6–glucose. Additional experiments revealed large variability in metabolite transformation and the key compounds, showing the bacterial accumulation of glutamate over the incubation period, and that of 3-hydroxybutyrate with increasing concentrations of 13C6–glucose added. These results suggest the potential ability of our approach to track substrate transformation in aquatic bacterial communities. Further applications of this procedure may provide substantial insights into the metabolite dynamics in aquatic environments. View Full-Text
Keywords: aquatic environment; heterotrophic bacteria; intracellular metabolite; metabolomics; nuclear magnetic resonance spectroscopy; stable-isotope labeling aquatic environment; heterotrophic bacteria; intracellular metabolite; metabolomics; nuclear magnetic resonance spectroscopy; stable-isotope labeling
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Uchimiya, M.; Tsuboi, Y.; Ito, K.; Date, Y.; Kikuchi, J. Bacterial Substrate Transformation Tracked by Stable-Isotope-Guided NMR Metabolomics: Application in a Natural Aquatic Microbial Community. Metabolites 2017, 7, 52.

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