Next Article in Journal / Special Issue
Methanol Generates Numerous Artifacts during Sample Extraction and Storage of Extracts in Metabolomics Research
Previous Article in Journal
Metabolomic Profiles of a Midge (Procladius villosimanus, Kieffer) Are Associated with Sediment Contamination in Urban Wetlands
Previous Article in Special Issue
Cell-Type Specific Metabolic Flux Analysis: A Challenge for Metabolic Phenotyping and a Potential Solution in Plants
Article Menu
Issue 4 (December) cover image

Export Article

Open AccessArticle
Metabolites 2017, 7(4), 65; https://doi.org/10.3390/metabo7040065

Dynamics of Monoterpene Formation in Spike Lavender Plants

1
Departamento de Biología Vegetal, ISIC/ERI de Biotecnología y Biomedicina BIOTECMED, Universidad de Valencia, Av. Vicent Andrés Estellés s/n, 46100 Burjasot, Valencia, Spain
2
Lehrstuhl für Biochemie, Technische Universität München, Lichtenbergstraße 4, 85748 Garching, Germany
*
Author to whom correspondence should be addressed.
Received: 24 October 2017 / Revised: 15 December 2017 / Accepted: 16 December 2017 / Published: 19 December 2017
(This article belongs to the Special Issue Isotope Guided Metabolomics and Flux Analysis)
View Full-Text   |   Download PDF [4082 KB, uploaded 19 December 2017]   |  

Abstract

The metabolic cross-talk between the mevalonate (MVA) and the methylerythritol phosphate (MEP) pathways was analyzed in spike lavender (Lavandula latifolia Med) on the basis of 13CO2-labelling experiments using wildtype and transgenic plants overexpressing the 3-hydroxy-3-methylglutaryl CoA reductase (HMGR), the first and key enzyme of the MVA pathway. The plants were labelled in the presence of 13CO2 in a gas chamber for controlled pulse and chase periods of time. GC/MS and NMR analysis of 1,8-cineole and camphor, the major monoterpenes present in their essential oil, indicated that the C5-precursors, isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP) of both monoterpenes are predominantly biosynthesized via the MEP pathway. Surprisingly, overexpression of HMGR did not have significant impact upon the crosstalk between the MVA and MEP pathways indicating that the MEP route is the preferred pathway for the synthesis of C5 monoterpene precursors in spike lavender. View Full-Text
Keywords: essential oils; isotopologue profiling; lamiaceae; Lavandula latifolia; spike lavender; terpenoid biosynthesis; mevalonate; CO2 essential oils; isotopologue profiling; lamiaceae; Lavandula latifolia; spike lavender; terpenoid biosynthesis; mevalonate; CO2
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Supplementary material

Share & Cite This Article

MDPI and ACS Style

Mendoza-Poudereux, I.; Kutzner, E.; Huber, C.; Segura, J.; Arrillaga, I.; Eisenreich, W. Dynamics of Monoterpene Formation in Spike Lavender Plants. Metabolites 2017, 7, 65.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Metabolites EISSN 2218-1989 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top