Special Issue "Molecular Cut and Paste"
Deadline for manuscript submissions: closed (15 October 2013)
Prof. Dr. Makoto Komiyama
Life Science Center of Tsukuba Advanced Research Alliance, University of Tsukuba, Ten-noudai 1-1-1, Tsukuba, Ibaraki 305-8577, Japan
Interests: DNA;RNA; hydrolysis;site-selective scission;gene manipulation
Prof. Dr. Weiguo Cao (Website)
Genetics and Biochemistry, Clemson University, Clemson, South Carolina, USA
Interests: DNA damage; mutagenesis and repair; evolution of repair enzymes; nuclease; DNA glycosylase; ligase; nucleic acids amplification and detection; protein engineering
Anyone who has used a word processing application knows the convenience of “cut”, “copy” and “paste”. It is unimaginable to manipulate words digitally without these clever functions. It turns out that the creation of “cut”, “copy” and “paste” is far more ancient than modern day word processing. Through evolution, a vast set of tools has been developed for molecular surgery much like the way we delete, duplicate, translocate, and connect words. The birth of biotechnology in the 1970’s–1980’s was the direct outcome of the understanding of restriction enzymes, DNA polymerases and DNA ligases and their ingenious uses. In living cells, a large set of elaborative tools has been developed to manipulate DNA molecules to meet the needs of cellular maintenance and reproduction, whether it is DNA replication, DNA repair or DNA degradation. Tools to cut other macromolecules such as RNA, proteins, carbohydrates and lipids have also been invented. In recent years, much progress has been made to understand and develop chemical and enzymological tools for molecular cutting and pasting. From an ever expanding list of native restriction enzymes to artificial chemical restriction enzymes; from DNA ligases to chemical ligation agents; from cut-paste-based cloning to topo-cloning; from protein splicing to site-specific protein tagging; from site-defined restriction endonucleases to site-designed zinc finger nucleases; these amazing tools are helping researchers to meet the needs of molecular biology, biotechnology, medicine and therapy, nanotechnology, and other new bioprocesses. This special issue is designed to be a collection of articles reporting the exciting progress in the broad areas of molecular cutting and pasting. Both original research articles and review articles in chemical and enzymological manipulations of DNA, RNA, carbohydrates and lipids are welcome.
Prof. Dr. Makoto Komiyama
Prof. Dr. Weiguo Cao
Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. Papers will be published continuously (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.
Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are refereed through a peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. International Journal of Molecular Sciences is an international peer-reviewed Open Access monthly journal published by MDPI.
- restriction endonuclease
- artificial restriction enzyme
- chemical ligation
- molecular cloning