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Tailoring the Models of Transcription
The Welcome Trust Sanger Institute, Genome Campus Hinxton, Cambridge CB10 1SA, UK
Received: 6 March 2013; in revised form: 22 March 2013 / Accepted: 26 March 2013 / Published: 8 April 2013
Abstract: Molecular biology is a rapidly evolving field that has led to the development of increasingly sophisticated technologies to improve our capacity to study cellular processes in much finer detail. Transcription is the first step in protein expression and the major point of regulation of the components that determine the characteristics, fate and functions of cells. The study of transcriptional regulation has been greatly facilitated by the development of reporter genes and transcription factor expression vectors, which have become versatile tools for manipulating promoters, as well as transcription factors in order to examine their function. The understanding of promoter complexity and transcription factor structure offers an insight into the mechanisms of transcriptional control and their impact on cell behaviour. This review focuses on some of the many applications of molecular cut-and-paste tools for the manipulation of promoters and transcription factors leading to the understanding of crucial aspects of transcriptional regulation.
Keywords: transcription; promoter; reporter gene; transcription factor; chimera
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Cite This Article
MDPI and ACS Style
Pance, A. Tailoring the Models of Transcription. Int. J. Mol. Sci. 2013, 14, 7583-7597.
Pance A. Tailoring the Models of Transcription. International Journal of Molecular Sciences. 2013; 14(4):7583-7597.
Pance, Alena. 2013. "Tailoring the Models of Transcription." Int. J. Mol. Sci. 14, no. 4: 7583-7597.