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Molecular Diversity Assessment Using Sequence Related Amplified Polymorphism (SRAP) Markers in Vicia faba L.
Legume Research Group, Plant production Department, Collage of Food and Agricultural Sciences, King Saud University, P.O. Box 2460, Riyadh 11451, Saudi Arabia
College of science and humanity studies, Salman Ibn Abdulaziz University, P.O. Box 83, Alkharj 11492, Saudi Arabia
* Author to whom correspondence should be addressed.
Received: 2 October 2012; in revised form: 9 November 2012 / Accepted: 12 November 2012 / Published: 4 December 2012
Abstract: Sequence-related amplified polymorphism (SRAP) markers were used to assess the genetic diversity and relationship among 58 faba bean (Vicia faba L.) genotypes. Fourteen SRAP primer combinations amplified a total of 1036 differently sized well-resolved peaks (fragments), of which all were polymorphic with a 0.96 PIC value and discriminated all of the 58 faba bean genotypes. An average pairwise similarity of 21% was revealed among the genotypes ranging from 2% to 65%. At a similarity of 28%, UPGMA clustered the genotypes into three main groups comprising 78% of the genotypes. The local landraces and most of the Egyptian genotypes in addition to the Sudan genotypes were grouped in the first main cluster. The advanced breeding lines were scattered in the second and third main clusters with breeding lines from the ICARDA and genotypes introduced from Egypt. At a similarity of 47%, all the genotypes formed separated clusters with the exceptions of Hassawi 1 and Hassawi 2. Group analysis of the genotypes according to their geographic origin and type showed that the landraces were grouped according to their origin, while others were grouped according to their seed type. To our knowledge, this is the first application of SRAP markers for the assessment of genetic diversity in faba bean. Such information will be useful to determine optimal breeding strategies to allow continued progress in faba bean breeding.
Keywords: Vicia faba L.; genetic diversity; germplasm collection; SRAP markers
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Alghamdi, S.S.; Al-Faifi, S.A.; Migdadi, H.M.; Khan, M.A.; EL-Harty, E.H.; Ammar, M.H. Molecular Diversity Assessment Using Sequence Related Amplified Polymorphism (SRAP) Markers in Vicia faba L.. Int. J. Mol. Sci. 2012, 13, 16457-16471.
Alghamdi SS, Al-Faifi SA, Migdadi HM, Khan MA, EL-Harty EH, Ammar MH. Molecular Diversity Assessment Using Sequence Related Amplified Polymorphism (SRAP) Markers in Vicia faba L.. International Journal of Molecular Sciences. 2012; 13(12):16457-16471.
Alghamdi, Salem S.; Al-Faifi, Sulieman A.; Migdadi, Hussein M.; Khan, Muhammad A.; EL-Harty, Ehab H.; Ammar, Megahed H. 2012. "Molecular Diversity Assessment Using Sequence Related Amplified Polymorphism (SRAP) Markers in Vicia faba L.." Int. J. Mol. Sci. 13, no. 12: 16457-16471.