Next Article in Journal
Exploiting BSA to Inhibit the Fibrous Aggregation of Magnetic Nanoparticles under an Alternating Magnetic Field
Next Article in Special Issue
Thermostable Mismatch-Recognizing Protein MutS Suppresses Nonspecific Amplification during Polymerase Chain Reaction (PCR)
Previous Article in Journal
Batch and Continuous Ultrasound Assisted Extraction of Boldo Leaves (Peumus boldus Mol.)
Previous Article in Special Issue
Cut-and-Paste of DNA Using an Artificial Restriction DNA Cutter
Int. J. Mol. Sci. 2013, 14(3), 5765-5774; doi:10.3390/ijms14035765
Article

Quick, Selective and Reversible Photocrosslinking Reaction between 5-Methylcytosine and 3-Cyanovinylcarbazole in DNA Double Strand

1,2,* , 1
 and 1
Received: 20 December 2012; in revised form: 12 February 2013 / Accepted: 26 February 2013 / Published: 12 March 2013
(This article belongs to the Special Issue Molecular Cut and Paste)
View Full-Text   |   Download PDF [1433 KB, uploaded 19 June 2014]   |   Browse Figures
Abstract: Selective photocrosslinking reaction between 3-cyanovinylcarbazole nucleoside (CNVK) and 5-methylcytosine (mC), which is known as epigenetic modification in genomic DNA, was developed. The reaction was completely finished within 5 s of 366 nm irradiation, and the rate of this photocrosslinking reaction was ca. 30-fold higher than that in the case of unmodified normal cytosine. There were no significant differences in the thermodynamic parameters and the kinetics of hybrid formation of oligonucleotide (ODN) containing CNVK and its complementary ODN containing C or mC at the photocrosslinking site, and suggesting that the quick and selective photoreaction has potential for the selective detection of mC in the DNA strand via the photocrosslinking reaction.
Keywords: photocrosslinking; 5-methylcytosine; 3-cyanovinylcarbazole nucleoside photocrosslinking; 5-methylcytosine; 3-cyanovinylcarbazole nucleoside
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Export to BibTeX |
EndNote


MDPI and ACS Style

Fujimo, K.; Konishi-Hiratsuka, K.; Sakamoto, T. Quick, Selective and Reversible Photocrosslinking Reaction between 5-Methylcytosine and 3-Cyanovinylcarbazole in DNA Double Strand. Int. J. Mol. Sci. 2013, 14, 5765-5774.

AMA Style

Fujimo K, Konishi-Hiratsuka K, Sakamoto T. Quick, Selective and Reversible Photocrosslinking Reaction between 5-Methylcytosine and 3-Cyanovinylcarbazole in DNA Double Strand. International Journal of Molecular Sciences. 2013; 14(3):5765-5774.

Chicago/Turabian Style

Fujimo, Kenzo; Konishi-Hiratsuka, Kaoru; Sakamoto, Takashi. 2013. "Quick, Selective and Reversible Photocrosslinking Reaction between 5-Methylcytosine and 3-Cyanovinylcarbazole in DNA Double Strand." Int. J. Mol. Sci. 14, no. 3: 5765-5774.


Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert