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Int. J. Mol. Sci. 2014, 15(2), 2773-2793; doi:10.3390/ijms15022773
Review

Bacterial Cellular Engineering by Genome Editing and Gene Silencing

1,2
 and
1,3,*
Received: 23 December 2013 / Revised: 27 January 2014 / Accepted: 28 January 2014 / Published: 18 February 2014
(This article belongs to the Special Issue Molecular Cut and Paste)
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Abstract

Genome editing is an important technology for bacterial cellular engineering, which is commonly conducted by homologous recombination-based procedures, including gene knockout (disruption), knock-in (insertion), and allelic exchange. In addition, some new recombination-independent approaches have emerged that utilize catalytic RNAs, artificial nucleases, nucleic acid analogs, and peptide nucleic acids. Apart from these methods, which directly modify the genomic structure, an alternative approach is to conditionally modify the gene expression profile at the posttranscriptional level without altering the genomes. This is performed by expressing antisense RNAs to knock down (silence) target mRNAs in vivo. This review describes the features and recent advances on methods used in genomic engineering and silencing technologies that are advantageously used for bacterial cellular engineering.
Keywords: gene knockout; allelic exchange; gene knock-in; genome editing; RNA-guided nucleases; mobile group II intron; antisense RNA; gene knockdown; gene silencing gene knockout; allelic exchange; gene knock-in; genome editing; RNA-guided nucleases; mobile group II intron; antisense RNA; gene knockdown; gene silencing
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Nakashima, N.; Miyazaki, K. Bacterial Cellular Engineering by Genome Editing and Gene Silencing. Int. J. Mol. Sci. 2014, 15, 2773-2793.

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