Special Issue "Immunosensors"
Deadline for manuscript submissions: closed (31 October 2010)
Prof. Dr. Loïc J. Blum
Université Claude Bernard Lyon1, Institut de Chimie et Biochimie Moléculaires et Supramoléculmaires, ICBMS-UMR5246 Bâtiment Curien, 43 Boulevard du 11 novembre 1918, F-69622 Villeurbanne Cédex, France
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Interests: chemi- and bio-luminescence; electrochemiluminescence; immunosensors; biochips; fiberoptic biosensors
Immunosensors are biosensors based on specific antigen-antibody interactions and in which the transducer detect either directly or indirectly the immunochemical reactions. In the indirect approach, the detection of the immune complex is achieved through the labelling of either the antibody or the antigen depending on the immunoassay format (sandwich type, competition, capture). Most often, an optical detection (fluorescence chemiluminescence, electrochemiluminescence, absorbance) is used although electrochemical detection has been also described. Direct detection approaches are label-free methods, in which the specific binding event between the antibody and the target analyte (the antigen) is monitored by a change or a variation in physicochemical properties. In that case, the detection methods include electrochemical impedance spectroscopy, microgravimetry and surface plasmon resonance (SPR). In addition, these direct detection methods can also provide kinetics information on the antigen-antibody reaction.
Beyond the specific detection of analytes, the constant search for high-performance systems has led with the help of micro and nanotechnologies and the integration of microfluidics to the development of miniaturized immunosensor-based devices with for some of them high-throughput analytical properties. Not only clinical analysis, the traditional field of application of immunoanalysis, will benefit from these new developments but also environmental analysis, quality control in pharmaceutical and food industries, biosecurity and prevention of bioterrorism and finally the proteomic era with protein profiling and protein-protein interaction studies.
Prof. Dr. Loïc Jacques Blum
- electrochemical immunosensors
- chemiluminescent immunosensors
- capacitive immunosensors
- protein immobilization
- Langmuir-Blodgett films
- total internal reflection fluorescence (TIRF)
- Fourier transform infrared (FTIR) immunosensors
- Surface Plasmon Resonance (SPR)
- label-free immunosensors
- antigen immobilization
- antibody immobilization
- protein chips
- flow immunosensors