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Sensors 2010, 10(12), 11287-11300; doi:10.3390/s101211287

Ca2+-Regulated Photoproteins: Effective Immunoassay Reporters

Institute of Biophysics, Siberian Branch of the Russian Academy of Sciences, Akademgorodok, 50, Krasnoyarsk, 660036, Russia
Received: 29 October 2010 / Revised: 24 November 2010 / Accepted: 3 December 2010 / Published: 10 December 2010
(This article belongs to the Special Issue Immunosensors)
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Abstract

Ca2+-regulated photoproteins of luminous marine coelenterates are of interest and a challenge for researchers as a unique bioluminescent system and as a promising analytical instrument for both in vivo and in vitro applications. The proteins are comprehensively studied as to biochemical properties, tertiary structures, bioluminescence mechanism, etc. This knowledge, along with available recombinant proteins serves the basis for development of unique bioluminescent detection systems that are “self-contained”, triggerable, fast, highly sensitive, and non-hazardous. In the paper, we focus on the use of photoproteins as reporters in binding assays based on immunological recognition element—bioluminescent immunoassay and hybridization immunoassay, their advantages and prospects.
Keywords: bioluminescence; Ca2+-regulated photoprotein; immunoassay; PCR-ELISA; multiplex assay; re-engineered photoproteins bioluminescence; Ca2+-regulated photoprotein; immunoassay; PCR-ELISA; multiplex assay; re-engineered photoproteins
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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Frank, L.A. Ca2+-Regulated Photoproteins: Effective Immunoassay Reporters. Sensors 2010, 10, 11287-11300.

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