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Sensors 2010, 10(3), 2045-2053; doi:10.3390/s100302045
Article

Signal Amplification by Enzymatic Reaction in an Immunosensor Based on Localized Surface Plasmon Resonance (LSPR)

1,2
,
1
,
1,2
,
1,2
,
1,2
 and
1,2,*
1 Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon 305-806, Korea 2 University of Science and Technology (UST), 305-333 Daejeon, Korea
* Author to whom correspondence should be addressed.
Received: 20 January 2010 / Revised: 15 February 2010 / Accepted: 4 March 2010 / Published: 12 March 2010
(This article belongs to the Special Issue Immunosensors)
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Abstract

An enzymatic reaction was employed as a means to enhance the sensitivity of an immunosensor based on localized surface plasmon resonance (LSPR). The reaction occurs after intermolecular binding between an antigen and an antibody on gold nano-island (NI) surfaces. For LSPR sensing, the gold NI surface was fabricated on glass substrates using vacuum evaporation and heat treatment. The interferon-g (IFN-g) capture antibody was immobilized on the gold NIs, followed by binding of IFN-g to the antibody. Subsequently, a biotinylated antibody and a horseradish peroxidase (HRP) conjugated with avidin were simultaneously introduced. A solution of 4-chloro-1-naphthol (4-CN) was then used for precipitation; precipitation was the result of the enzymatic reaction catalyzed the HRP on gold NIs. The LSPR spectra were obtained after each binding process. Using this method, the enzyme-catalyzed precipitation reaction on the gold NI surface was found to effectively amplify the change in the signal of the LSPR immunosensor after intermolecular binding.
Keywords: immunosensor; localized surface plasmon resonance (LSPR); gold nano-island; enzyme-catalyzed precipitation immunosensor; localized surface plasmon resonance (LSPR); gold nano-island; enzyme-catalyzed precipitation
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).
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Lee, T.-H.; Lee, S.-W.; Jung, J.-A.; Ahn, J.; Kim, M.-G.; Shin, Y.-B. Signal Amplification by Enzymatic Reaction in an Immunosensor Based on Localized Surface Plasmon Resonance (LSPR). Sensors 2010, 10, 2045-2053.

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