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Signal Amplification by Enzymatic Reaction in an Immunosensor Based on Localized Surface Plasmon Resonance (LSPR)
Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon 305-806, Korea
University of Science and Technology (UST), 305-333 Daejeon, Korea
* Author to whom correspondence should be addressed.
Received: 20 January 2010; in revised form: 15 February 2010 / Accepted: 4 March 2010 / Published: 12 March 2010
Abstract: An enzymatic reaction was employed as a means to enhance the sensitivity of an immunosensor based on localized surface plasmon resonance (LSPR). The reaction occurs after intermolecular binding between an antigen and an antibody on gold nano-island (NI) surfaces. For LSPR sensing, the gold NI surface was fabricated on glass substrates using vacuum evaporation and heat treatment. The interferon-g (IFN-g) capture antibody was immobilized on the gold NIs, followed by binding of IFN-g to the antibody. Subsequently, a biotinylated antibody and a horseradish peroxidase (HRP) conjugated with avidin were simultaneously introduced. A solution of 4-chloro-1-naphthol (4-CN) was then used for precipitation; precipitation was the result of the enzymatic reaction catalyzed the HRP on gold NIs. The LSPR spectra were obtained after each binding process. Using this method, the enzyme-catalyzed precipitation reaction on the gold NI surface was found to effectively amplify the change in the signal of the LSPR immunosensor after intermolecular binding.
Keywords: immunosensor; localized surface plasmon resonance (LSPR); gold nano-island; enzyme-catalyzed precipitation
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MDPI and ACS Style
Lee, T.-H.; Lee, S.-W.; Jung, J.-A.; Ahn, J.; Kim, M.-G.; Shin, Y.-B. Signal Amplification by Enzymatic Reaction in an Immunosensor Based on Localized Surface Plasmon Resonance (LSPR). Sensors 2010, 10, 2045-2053.
Lee T-H, Lee S-W, Jung J-A, Ahn J, Kim M-G, Shin Y-B. Signal Amplification by Enzymatic Reaction in an Immunosensor Based on Localized Surface Plasmon Resonance (LSPR). Sensors. 2010; 10(3):2045-2053.
Lee, Tae-Han; Lee, Seung-Woo; Jung, Ji-Ae; Ahn, Junhyoung; Kim, Min-Gon; Shin, Yong-Beom. 2010. "Signal Amplification by Enzymatic Reaction in an Immunosensor Based on Localized Surface Plasmon Resonance (LSPR)." Sensors 10, no. 3: 2045-2053.