Abstract: An enzymatic reaction was employed as a means to enhance the sensitivity of an immunosensor based on localized surface plasmon resonance (LSPR). The reaction occurs after intermolecular binding between an antigen and an antibody on gold nano-island (NI) surfaces. For LSPR sensing, the gold NI surface was fabricated on glass substrates using vacuum evaporation and heat treatment. The interferon-g (IFN-g) capture antibody was immobilized on the gold NIs, followed by binding of IFN-g to the antibody. Subsequently, a biotinylated antibody and a horseradish peroxidase (HRP) conjugated with avidin were simultaneously introduced. A solution of 4-chloro-1-naphthol (4-CN) was then used for precipitation; precipitation was the result of the enzymatic reaction catalyzed the HRP on gold NIs. The LSPR spectra were obtained after each binding process. Using this method, the enzyme-catalyzed precipitation reaction on the gold NI surface was found to effectively amplify the change in the signal of the LSPR immunosensor after intermolecular binding.
Keywords: immunosensor; localized surface plasmon resonance (LSPR); gold nano-island; enzyme-catalyzed precipitation
This is an open access article distributed under the
Creative Commons Attribution License which permits unrestricted use, distribution,
and reproduction in any medium, provided the original work is properly cited.
Export to BibTeX
MDPI and ACS Style
Lee, T.-H.; Lee, S.-W.; Jung, J.-A.; Ahn, J.; Kim, M.-G.; Shin, Y.-B. Signal Amplification by Enzymatic Reaction in an Immunosensor Based on Localized Surface Plasmon Resonance (LSPR). Sensors 2010, 10, 2045-2053.
Lee T-H, Lee S-W, Jung J-A, Ahn J, Kim M-G, Shin Y-B. Signal Amplification by Enzymatic Reaction in an Immunosensor Based on Localized Surface Plasmon Resonance (LSPR). Sensors. 2010; 10(3):2045-2053.
Lee, Tae-Han; Lee, Seung-Woo; Jung, Ji-Ae; Ahn, Junhyoung; Kim, Min-Gon; Shin, Yong-Beom. 2010. "Signal Amplification by Enzymatic Reaction in an Immunosensor Based on Localized Surface Plasmon Resonance (LSPR)." Sensors 10, no. 3: 2045-2053.