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Sensors 2010, 10(5), 5160-5170; doi:10.3390/s100505160

A Highly Sensitive Enzyme-Amplified Immunosensor Based on a Nanoporous Niobium Oxide (Nb2O5) Electrode

1 BioNanotechnology Research Center (BNRC), Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon 305-333, Korea 2 Department of Chemical Engineering, Inha University, 253 Yonghyun-Dong, Nam-Gu, Incheon 402-751, Korea
* Author to whom correspondence should be addressed.
Received: 26 March 2010 / Revised: 13 April 2010 / Accepted: 10 May 2010 / Published: 25 May 2010
(This article belongs to the Special Issue Immunosensors)
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We report on the development of an enzyme-amplified sandwich-type immunosensor based on a thin gold film sputtered on an anodic nanoporous niobium oxide (Au@Nb2O5) electrode. The electrocatalytic activity of enzymatically amplified electroactive species and a stable electrode consisting of Au@Nb2O5 were used to obtain a powerful signal amplification of the electrochemical immunobiosensor. The method using this electrochemical biosensor based on an Au@Nb2O5 electrode provides a much better performance than those based on conventional bulk gold or niobium oxide electrodes. Our novel approach does not require any time-consuming cleaning steps to yield reproducible electrochemical signals. In addition, the strong adhesion of gold films on the niobium oxide electrodes offers a very stable substrate during electrochemical biosensing. Cyclic voltammetry measurements indicate that non-specific binding of proteins to the modified Au@Nb2O5 surface is sufficiently low to be ignored in the case of our novel system. Finally, we demonstrated the ability of the biosensor based on an Au@Nb2O5 offering the enhanced performance with a high resolution and sensitivity. Therefore, it is expected that the biosensor based on an Au@Nb2O5 has great potential for highly efficient biological devices.
Keywords: electrochemical biosensor; niobium oxide; enzyme; immunosensor electrochemical biosensor; niobium oxide; enzyme; immunosensor
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Lee, C.-S.; Kwon, D.; Yoo, J.E.; Lee, B.G.; Choi, J.; Chung, B.H. A Highly Sensitive Enzyme-Amplified Immunosensor Based on a Nanoporous Niobium Oxide (Nb2O5) Electrode. Sensors 2010, 10, 5160-5170.

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