Next Article in Journal
Location Estimation in Wireless Sensor Networks Using Spring-Relaxation Technique
Next Article in Special Issue
Prospects of Nanotechnology in Clinical Immunodiagnostics
Previous Article in Journal
Carbon Nanostructure-Based Field-Effect Transistors for Label-Free Chemical/Biological Sensors
Previous Article in Special Issue
Multiplexed Electrochemical Detection of Yersinia Pestis and Staphylococcal Enterotoxin B using an Antibody Microarray
Article Menu

Export Article

Open AccessArticle
Sensors 2010, 10(5), 5160-5170; doi:10.3390/s100505160

A Highly Sensitive Enzyme-Amplified Immunosensor Based on a Nanoporous Niobium Oxide (Nb2O5) Electrode

BioNanotechnology Research Center (BNRC), Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon 305-333, Korea
Department of Chemical Engineering, Inha University, 253 Yonghyun-Dong, Nam-Gu, Incheon 402-751, Korea
Author to whom correspondence should be addressed.
Received: 26 March 2010 / Revised: 13 April 2010 / Accepted: 10 May 2010 / Published: 25 May 2010
(This article belongs to the Special Issue Immunosensors)
View Full-Text   |   Download PDF [1033 KB, uploaded 21 June 2014]   |  


We report on the development of an enzyme-amplified sandwich-type immunosensor based on a thin gold film sputtered on an anodic nanoporous niobium oxide (Au@Nb2O5) electrode. The electrocatalytic activity of enzymatically amplified electroactive species and a stable electrode consisting of Au@Nb2O5 were used to obtain a powerful signal amplification of the electrochemical immunobiosensor. The method using this electrochemical biosensor based on an Au@Nb2O5 electrode provides a much better performance than those based on conventional bulk gold or niobium oxide electrodes. Our novel approach does not require any time-consuming cleaning steps to yield reproducible electrochemical signals. In addition, the strong adhesion of gold films on the niobium oxide electrodes offers a very stable substrate during electrochemical biosensing. Cyclic voltammetry measurements indicate that non-specific binding of proteins to the modified Au@Nb2O5 surface is sufficiently low to be ignored in the case of our novel system. Finally, we demonstrated the ability of the biosensor based on an Au@Nb2O5 offering the enhanced performance with a high resolution and sensitivity. Therefore, it is expected that the biosensor based on an Au@Nb2O5 has great potential for highly efficient biological devices. View Full-Text
Keywords: electrochemical biosensor; niobium oxide; enzyme; immunosensor electrochemical biosensor; niobium oxide; enzyme; immunosensor

This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Lee, C.-S.; Kwon, D.; Yoo, J.E.; Lee, B.G.; Choi, J.; Chung, B.H. A Highly Sensitive Enzyme-Amplified Immunosensor Based on a Nanoporous Niobium Oxide (Nb2O5) Electrode. Sensors 2010, 10, 5160-5170.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics



[Return to top]
Sensors EISSN 1424-8220 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top