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A Highly Sensitive Enzyme-Amplified Immunosensor Based on a Nanoporous Niobium Oxide (Nb2O5) Electrode
BioNanotechnology Research Center (BNRC), Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon 305-333, Korea
Department of Chemical Engineering, Inha University, 253 Yonghyun-Dong, Nam-Gu, Incheon 402-751, Korea
* Author to whom correspondence should be addressed.
Received: 26 March 2010; in revised form: 13 April 2010 / Accepted: 10 May 2010 / Published: 25 May 2010
Abstract: We report on the development of an enzyme-amplified sandwich-type immunosensor based on a thin gold film sputtered on an anodic nanoporous niobium oxide (Au@Nb2O5) electrode. The electrocatalytic activity of enzymatically amplified electroactive species and a stable electrode consisting of Au@Nb2O5 were used to obtain a powerful signal amplification of the electrochemical immunobiosensor. The method using this electrochemical biosensor based on an Au@Nb2O5 electrode provides a much better performance than those based on conventional bulk gold or niobium oxide electrodes. Our novel approach does not require any time-consuming cleaning steps to yield reproducible electrochemical signals. In addition, the strong adhesion of gold films on the niobium oxide electrodes offers a very stable substrate during electrochemical biosensing. Cyclic voltammetry measurements indicate that non-specific binding of proteins to the modified Au@Nb2O5 surface is sufficiently low to be ignored in the case of our novel system. Finally, we demonstrated the ability of the biosensor based on an Au@Nb2O5 offering the enhanced performance with a high resolution and sensitivity. Therefore, it is expected that the biosensor based on an Au@Nb2O5 has great potential for highly efficient biological devices.
Keywords: electrochemical biosensor; niobium oxide; enzyme; immunosensor
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Lee, C.-S.; Kwon, D.; Yoo, J.E.; Lee, B.G.; Choi, J.; Chung, B.H. A Highly Sensitive Enzyme-Amplified Immunosensor Based on a Nanoporous Niobium Oxide (Nb2O5) Electrode. Sensors 2010, 10, 5160-5170.
Lee C-S, Kwon D, Yoo JE, Lee BG, Choi J, Chung BH. A Highly Sensitive Enzyme-Amplified Immunosensor Based on a Nanoporous Niobium Oxide (Nb2O5) Electrode. Sensors. 2010; 10(5):5160-5170.
Lee, Chang-Soo; Kwon, Dohyoung; Yoo, Jeng Eun; Lee, Byung Gun; Choi, Jinsub; Chung, Bong Hyun. 2010. "A Highly Sensitive Enzyme-Amplified Immunosensor Based on a Nanoporous Niobium Oxide (Nb2O5) Electrode." Sensors 10, no. 5: 5160-5170.