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• Wojciechowski, J
• Danley, D
• Cooper, J
• Yazvenko, N
• Taitt, C
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• Wojciechowski, J
• Danley, D
• Cooper, J
• Yazvenko, N
• Taitt, C
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• Wojciechowski, J
• Danley, D
• Cooper, J
• Yazvenko, N
• Taitt, C

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Sensors 2010, 10(4), 3351-3362; doi:10.3390/s100403351

Article

Multiplexed Electrochemical Detection of Yersinia Pestis and Staphylococcal Enterotoxin B using an Antibody Microarray

Jason Wojciechowski ¹ , David Danley ² , John Cooper ² , Nina Yazvenko ²  and Chris Rowe Taitt ^1,*

¹ Center for Bio, Molecular Science and Engineering, Bld. 30, US Naval Research Laboratory, 4555 Overlook Ave. SW, Washington DC 20376, USA ² CombiMatrix Corporation, 6500 Harbour Heights Pkwy., Suite #303, Mukilteo, WA 98275, USA

* Author to whom correspondence should be addressed.

Received: 8 February 2010; in revised form: 28 February 2010 / Accepted: 26 March 2010 / Published: 6 April 2010

(This article belongs to the Special Issue Immunosensors)

Download PDF Full-Text [411 KB, uploaded 6 April 2010 13:28 CEST]

Abstract: The CombiMatrix antibody microarray is a versatile, sensitive detection platform based on the generation and transduction of electrochemical signals following antigen binding to surface antibodies. The sensor chip described herein is comprised of microelectrodes coupled to an adjacent bio-friendly matrix coated with antibodies to the biological pathogens Yersinia pestis and Bacillus anthracis, and the bacterial toxin staphylococcal enterotoxin B (SEB). Using this system, we were able to detect SEB and inactivated Y. pestis individually as well as in two-plex assays at concentrations as low as 5 pg/mL and 10⁶ CFU/mL, respectively. We also introduce super avidin-biotin system (SABS) as a viable and effective means to enhance assay signal responses and lower detection limits. Together these technologies represent substantial advances in point-of-care and point-of-use detection applications.

Keywords: CombiMatrix; microarray; biosensor; electrochemical detection; super avidin-biotin system (SABS); immunoassay; pathogen detection

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