Next Article in Journal
Next Article in Special Issue
Previous Article in Journal
Previous Article in Special Issue
Sensors 2010, 10(4), 3351-3362; doi:10.3390/s100403351
Article

Multiplexed Electrochemical Detection of Yersinia Pestis and Staphylococcal Enterotoxin B using an Antibody Microarray

1
, 2
, 2
, 2
 and 1,*
Received: 8 February 2010; in revised form: 28 February 2010 / Accepted: 26 March 2010 / Published: 6 April 2010
(This article belongs to the Special Issue Immunosensors)
View Full-Text   |   Download PDF [411 KB, uploaded 21 June 2014]
Abstract: The CombiMatrix antibody microarray is a versatile, sensitive detection platform based on the generation and transduction of electrochemical signals following antigen binding to surface antibodies. The sensor chip described herein is comprised of microelectrodes coupled to an adjacent bio-friendly matrix coated with antibodies to the biological pathogens Yersinia pestis and Bacillus anthracis, and the bacterial toxin staphylococcal enterotoxin B (SEB). Using this system, we were able to detect SEB and inactivated Y. pestis individually as well as in two-plex assays at concentrations as low as 5 pg/mL and 106 CFU/mL, respectively. We also introduce super avidin-biotin system (SABS) as a viable and effective means to enhance assay signal responses and lower detection limits. Together these technologies represent substantial advances in point-of-care and point-of-use detection applications.
Keywords: CombiMatrix; microarray; biosensor; electrochemical detection; super avidin-biotin system (SABS); immunoassay; pathogen detection CombiMatrix; microarray; biosensor; electrochemical detection; super avidin-biotin system (SABS); immunoassay; pathogen detection
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Export to BibTeX |
EndNote


MDPI and ACS Style

Wojciechowski, J.; Danley, D.; Cooper, J.; Yazvenko, N.; Taitt, C.R. Multiplexed Electrochemical Detection of Yersinia Pestis and Staphylococcal Enterotoxin B using an Antibody Microarray. Sensors 2010, 10, 3351-3362.

AMA Style

Wojciechowski J, Danley D, Cooper J, Yazvenko N, Taitt CR. Multiplexed Electrochemical Detection of Yersinia Pestis and Staphylococcal Enterotoxin B using an Antibody Microarray. Sensors. 2010; 10(4):3351-3362.

Chicago/Turabian Style

Wojciechowski, Jason; Danley, David; Cooper, John; Yazvenko, Nina; Taitt, Chris Rowe. 2010. "Multiplexed Electrochemical Detection of Yersinia Pestis and Staphylococcal Enterotoxin B using an Antibody Microarray." Sensors 10, no. 4: 3351-3362.



Sensors EISSN 1424-8220 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert