Special Issue "Fluorescent Biosensors"
Deadline for manuscript submissions: closed (31 October 2013)
Dr. Hui-Wang Ai
Department of Chemistry, 501 Big Springs Road, University of California, Riverside, CA 92521, USA
Interests: fluorescent sensors; environmental chemical biology; protein engineering and protein chemistry
The past a few decades have witnessed extraordinary advances in fluorescent biosensors that have revolutionized the way how biology could be studied. Fluorescent biosensors are molecules and devices that measure the concentrations, locations and other dynamics of biomolecules and bioactivities by means of fluorescence. Often these probes are coupled with state-of-the-art instruments including various microscopes and macroscopes to enable the imaging of cells, tissues, and intact multicellular organisms (e.g., plants, animals and human beings).
Fluorescent biosensors are usually based on fluorescent organic molecules, nanoparticles, proteins, or combinations of organic molecules, nanoparticles and proteins. They are designed and engineered to change their fluorescent colors or intensities in response to external stimuli or physiological changes including pH fluctuations, metal ion homeostasis, cell signaling, membrane potential differences, phosphorylation, ubiquitination, redox reactions, and apoptosis. In particular, the 2008 Nobel Prize in Chemistry was awarded to three scientists who discovered and developed fluorescent proteins, which have now been developed into a very large group of biosensors.
The aim of this special issue is to highlight high-quality results (including original research articles and reviews) in the field of fluorescent biosensor. Articles that focus on or propose new ideas and new directions are particularly welcome.
Dr. Huiwang Ai
Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. Papers will be published continuously (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.
Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are refereed through a peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Sensors is an international peer-reviewed Open Access monthly journal published by MDPI.
- fluorescent sensor
- ratiometric sensor
- intensiometric sensor
- fluorescent protein
- synthetic probe
- quantum dot
- optogenetic reporter
- genetically encoded probe
- reaction-based fluorescent sensor
Sensors 2013, 13(5), 5937-5944; doi:10.3390/s130505937
Received: 28 March 2013; in revised form: 25 April 2013 / Accepted: 5 May 2013 / Published: 10 May 2013| Download PDF Full-text (430 KB) | Download XML Full-text
Sensors 2013, 13(9), 11507-11521; doi:10.3390/s130911507
Received: 11 August 2013; in revised form: 27 August 2013 / Accepted: 29 August 2013 / Published: 2 September 2013| Download PDF Full-text (1888 KB) | Download XML Full-text | Supplementary Files
Article: Temporal and Spatial Properties of a Yeast Multi-Cellular Amplification System Based on Signal Molecule Diffusion
Sensors 2013, 13(11), 14511-14522; doi:10.3390/s131114511
Received: 22 August 2013; in revised form: 11 October 2013 / Accepted: 18 October 2013 / Published: 25 October 2013| Download PDF Full-text (817 KB) | Supplementary Files
Sensors 2013, 13(11), 15422-15433; doi:10.3390/s131115422
Received: 19 October 2013; in revised form: 2 November 2013 / Accepted: 5 November 2013 / Published: 11 November 2013| Download PDF Full-text (593 KB)
Article: Noninvasive High-Throughput Single-Cell Analysis of HIV Protease Activity Using Ratiometric Flow Cytometry
Sensors 2013, 13(12), 16330-16346; doi:10.3390/s131216330
Received: 18 October 2013; in revised form: 11 November 2013 / Accepted: 13 November 2013 / Published: 28 November 2013| Download PDF Full-text (1081 KB)
Review: Illumination of the Spatial Order of Intracellular pH by Genetically Encoded pH-Sensitive Sensors
Sensors 2013, 13(12), 16736-16758; doi:10.3390/s131216736
Received: 12 November 2013; in revised form: 27 November 2013 / Accepted: 27 November 2013 / Published: 5 December 2013| Download PDF Full-text (857 KB)
The below list represents only planned manuscripts. Some of these manuscripts have not been received by the Editorial Office yet. Papers submitted to MDPI journals are subject to peer-review.
Type of Paper: Article
Title: Signaling Properties of a Yeast Pheromone-Based Cellular Amplification System
Authors: Michael Jahn †, Annett Mölle †, Gerhard Rödel and Kai Ostermann
Affiliations: Institute of Genetics, Technische Universität Dresden, 01062 Dresden, Germany; E-Mail: firstname.lastname@example.org
† Authors with equal contributions.
Abstract: In order to advance a recently described yeast pheromone-based cell communication and amplifier system , we examined its spatial properties in more detail. It utilizes elements of the Saccharomyces cerevisiae mating response pathway and relies on diffusion of the pheromone α–factor as the key signaling molecule. The diffusion property of the pheromone was studied in an agarose hydrogel matrix consisting of two distinct compartments with a shared boundary. One compartment contained α-factor, either synthetic or native, and the other yeast cells responding to the pheromone by forming mating projections (“shmoo”) and expressing enhanced green fluorescent protein (EGFP) as a reporter.
Shmoo formation and fluorescence by the reporter cells was observed in a gradual manner up to a maximal distance of 3 mm indicating a dynamic α–factor gradient. According to a mathematical prediction, a distance of 5 mm can be bridged by pheromone diffusion in 1% agarose. The performance of the assay to measure pheromone response was improved by employing the reporter TurboRFP as one of the brightest red fluorescent proteins, and a 3D two-compartment setup. Even with a 1:20 ratio of α–factor secreting to reporter cells a distinct fluorescence signal can be generated. When using a ratio of 1:1, the secreted pheromone induced fluorescence in a distance of up to 4 mm after six hours. We conclude from both our experimental results and the mathematical diffusion model that the maximum dimension of a compartment should not exceed 5 mm in gradient direction. A spatial separation of different cell types linked by pheromone signaling is an ideal way to amplify, diversify, or detect the original signal. Our data demonstrate the potential of pheromone-driven gene expression in yeast for technical implementation of the modular, multi-cellular signaling and amplification system.
Reference: 1. Gross, A.; Rödel, G.; Ostermann, K. Application of the yeast pheromone system for controlled cell-cell communication and signal amplification. Lett. Appl. Microbiol. 2011, 52, 521-536.
Last update: 12 August 2013