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Genetically Encoded Fluorescent Redox Probes
Department of Chemistry, University of California, 501 Big Springs Road, Riverside, CA 92521, USA
* Author to whom correspondence should be addressed.
Received: 19 October 2013; in revised form: 2 November 2013 / Accepted: 5 November 2013 / Published: 11 November 2013
Abstract: Redox processes are involved in almost every cell of the body as a consequence of aerobic life. In the past decades, redox biology has been increasingly recognized as one of the key themes in cell signaling. The progress has been accelerated by development of fluorescent probes that can monitor redox conditions and dynamics in cells and cell compartments. This short paper focuses on fluorescent redox probes that are genetically encoded, and discusses their properties, molecular mechanism, advantages and pitfalls. Our recent work on reaction-based encoded probes that are responsive to particular redox signaling molecules is also reviewed. Future challenges and directions are also commented.
Keywords: redox signaling; fluorescent protein (FP)-based sensors; reaction-based probes; unnatural amino acids (UAAs); redox probes
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MDPI and ACS Style
Ren, W.; Ai, H.-W. Genetically Encoded Fluorescent Redox Probes. Sensors 2013, 13, 15422-15433.
Ren W, Ai H-W. Genetically Encoded Fluorescent Redox Probes. Sensors. 2013; 13(11):15422-15433.
Ren, Wei; Ai, Hui-Wang. 2013. "Genetically Encoded Fluorescent Redox Probes." Sensors 13, no. 11: 15422-15433.