Special Issue "Feature Papers"

A special issue of Methods and Protocols (ISSN 2409-9279).

Deadline for manuscript submissions: 30 June 2018

Special Issue Editor

Guest Editor
Prof. Fernando Albericio

1. School of Chemistry, University of KwaZulu-Natal, Durban 4001, South Africa
2. Department of Organic Chemistry, University of Barcelona, 08028-Barcelona, Spain
Website1 | Website2 | E-Mail
Fax: +34 93 403 71 26
Interests: solid-phase chemistry, combinatorial chemistry, drug delivery systems, peptides, peptide drug conjugates, orthogonal chemistry, drug discovery, biomaterials

Special Issue Information

Dear Colleagues,

As Editor-in-Chief of Methods and Protocols, I am glad to announce the Special Issue "Feature Papers". This Special Issue will be a collection of articles from top researchers describing new approaches or new cutting-edge developments in the fields of Life Sciences, Chemistry, and Biomedical Sciences, as well as in other relevant scientific fields. We welcome the submission of manuscripts from Editorial Board Members and from outstanding scholars invited by the Editorial Board and the Editorial Office.

You are welcome to send short proposals for submissions of Feature Papers to our Editorial Office (mps@mdpi.com) for evaluation.

Prof. Fernando Albericio
Editor-in-Chief and Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All papers will be peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Methods and Protocols is an international peer-reviewed open access quarterly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) is waived for well-prepared manuscripts submitted to this issue. Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.


Published Papers (1 paper)

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Research

Open AccessArticle Predictable Peptide Conjugation Ratios by Activation of Proteins with Succinimidyl Iodoacetate (SIA)
Methods Protoc. 2017, 1(1), 2; doi:10.3390/mps1010002
Received: 2 August 2017 / Revised: 12 September 2017 / Accepted: 14 September 2017 / Published: 25 September 2017
PDF Full-text (1868 KB) | HTML Full-text | XML Full-text
Abstract
The small heterobifunctional linker succinimidyl iodoacetate (SIA) was examined for the preparation of peptide–protein bioconjugates with predicable conjugation ratios. For many conjugation protocols, the protein is either treated with a reductant to cleave disulfide bonds or is reacted with thiolation chemicals, such as
[...] Read more.
The small heterobifunctional linker succinimidyl iodoacetate (SIA) was examined for the preparation of peptide–protein bioconjugates with predicable conjugation ratios. For many conjugation protocols, the protein is either treated with a reductant to cleave disulfide bonds or is reacted with thiolation chemicals, such as Traut’s reagent. Both approaches are difficult to control, need individual optimization and often lead to unsatisfactory results. In another popular approach, a heterobifunctional linker with a N-hydroxysuccinimide (NHS) and a maleimide functionality is applied to the protein. After the activation of some lysine ε-amino groups with the NHS ester functionality, a cysteine-containing peptide is attached to the activated carrier protein via maleimide. Particularly, the maleimide reaction leads to some unwanted byproducts or even cleavage of the linker. Many protocols end up with conjugates with unpredictable and irreproducible conjugation ratios. In addition, the maleimide-thiol addition product should be assumed immunogenic in vivo. To avoid these and other disadvantages of the maleimide approach, we examined the known linker succinimidyl iodoacetate (SIA) in more detail and developed two protocols, which lead to peptide–protein conjugates with predefined average conjugation ratios. This holds potential to eliminate tedious and expensive optimization steps for the synthesis of a bioconjugate of optimal composition. Full article
(This article belongs to the Special Issue Feature Papers)
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