Special Issue "Frontier of Protein Crystallography"
Deadline for manuscript submissions: 31 July 2019
Prof. Dr. Silvano Geremia
CEB Centre of Excellence in Biocrystallography, Department of Chemical and Pharmaceutical Sciences, University of Trieste, Via L. Giorgieri 1, Trieste, Italy
Interests: crystallography; structural biology; vitamin B12 proteins; redox-proteins; drug delivery; diagnostics; metals in medicine; bioinorganic chemistry; supramolecular chemistry; nanostructures
Structural biology is a relatively young science, initiated about 60 years ago with the elucidation of the first three-dimensional crystal structure of myoglobin. Protein crystallography continues to develop vigorously and today there are over 120,000 structures deposited at the Protein Data Bank (PDB), 90% of which are from X-ray data. The technological developments behind this rapid growth involves all crucial steps of the pipeline “from gene to structure”: from the developments of wet lab technologies, including recombinant DNA techniques, protein purification and crystallization; to innovative hardware technology, for example brilliant light sources such as synchrotrons and X-ray free electron lasers (XFEL) together with high-frame-rate and ultra-sensitive detectors. An important impetus to protein crystallography has also been provided by soft technology: theoretical foundations, computational algorithm, and software development. The bio-crystallography integrated with cryo-electron microscopy is a major research trend in structural biology and the present Special Issue is aimed at covering frontier technologies and methodologies in protein crystallography, as well as their novel applications.
Prof. Dr. Silvano Geremia
Manuscript Submission Information
Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All papers will be peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.
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- X-ray crystallography
- recombinant protein overexpression
- protein purification
- protein crystallization
- radiation sources
- X-ray detectors
- structural biology software
- high-throughput crystallography
- structural biology
The below list represents only planned manuscripts. Some of these manuscripts have not been received by the Editorial Office yet. Papers submitted to MDPI journals are subject to peer-review.
Author: Elspeth Garman and Martin Weik
Affiliation: Laboratory of Molecular Biophysics, Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, UK
Abstract: Eighteen years of published research on radiation damage to samples held around 100 K during X-ray crystallography has resulted in thorough characterisation of the phenomenon. However, there are still perplexing questions left to be answered in terms of understanding some of the documented effects, and before crystal clear guidance can be given to experimenters on how to minimise them.
This short review will summarise the main observations and remaining issues to be investigated, as well as some new developments coming into main-line diffraction experimental methods at synchrotrons to optimise the information that can be obtained from protein crystals.