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Special Issue "Analysis of Peptides and Proteins by Electrophoretic Techniques"

A special issue of Molecules (ISSN 1420-3049). This special issue belongs to the section "Analytical Chemistry".

Deadline for manuscript submissions: closed (31 July 2018)

Special Issue Editors

Guest Editor
Prof. Dr. Angela R. Piergiovanni

Istituto di Bioscienze e Biorisorse (IBBR-CNR), via Amendola 165/a, 70126 Bari, Italy
Website | E-Mail
Interests: electrophoretic techniques (CE, SDS-PAGE and PAGE); sequence analysis (including DNA sequences); genetic resources; seed quality; proteins; analytical and bioanalytical chemistry
Guest Editor
Prof. Dr. José Manuel Herrero-Martínez

Department of Analytical Chemistry, Faculty of Chemistry, University of Valencia, Burjassot, Spain
Website | E-Mail
Interests: porous materials; nanomaterials; molecular recognition sorbents; sample treatment; solid-phase extraction; capillary electromigration techniques; capillary/nano liquid chromatography; food analysis

Special Issue Information

Dear Colleagues,

The characterization of real complex matrices containing peptides and proteins constitutes a relevant issue in the research of life and biological sciences. To understand their functions in animals, plants, and food, studies of their expression levels, post-translational modifications, and specific interactions are crucial. Moreover, proteins and peptides are important biomarkers of many human diseases. Increase in our knowledge on this subject could help to develop biopharmaceuticals. Separation techniques, based on electromigration, coupled to sensitive detection methodologies, have enabled researchers to address these challenging issues. Recently, miniaturized electromigration techniques, including separations achieved in narrow fused silica capillaries or other microdevices, have increased separation efficiency and speed of analysis, reducing sample consumption and waste production.

The present Special Issue, “Analysis of Peptides and Proteins by Electrophoretic Techniques”, aims to collect and to spread some of the most significant and recent advancements in the study of theses macromolecules. The scope is broad, regarding the use of capillary electrophoresis (CE), chip-based and other miniaturized supports, as well as slab gels to characterize complex mixtures of peptides and proteins. Relevant applications to real matrices (biological fluids, plant extracts, food, etc.) and new technological developments such as microfluidics hyphenated with mass spectrometry, as well as future directions of these techniques, are welcomed.

Prof. Dr. Angela R. Piergiovanni
Prof. Dr. José Manuel Herrero-Martínez
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All papers will be peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Molecules is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 1800 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.


  • slab-gel electrophoresis
  • capillary electromigration techniques
  • chip based and capillary based CE
  • protein and/or peptides mixtures characterization
  • hyphenation with mass spectrometry
  • proteomics
  • metabolomics

Published Papers (1 paper)

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Open AccessCommunication First Look at the Venom of Naja ashei
Molecules 2018, 23(3), 609; https://doi.org/10.3390/molecules23030609
Received: 31 January 2018 / Revised: 2 March 2018 / Accepted: 6 March 2018 / Published: 8 March 2018
PDF Full-text (913 KB) | HTML Full-text | XML Full-text | Supplementary Files
Naja ashei is an African spitting cobra species closely related to N. mossambica and N. nigricollis. It is known that the venom of N. ashei, like that of other African spitting cobras, mainly has cytotoxic effects, however data about its specific
[...] Read more.
Naja ashei is an African spitting cobra species closely related to N. mossambica and N. nigricollis. It is known that the venom of N. ashei, like that of other African spitting cobras, mainly has cytotoxic effects, however data about its specific protein composition are not yet available. Thus, an attempt was made to determine the venom proteome of N. ashei with the use of 2-D electrophoresis and MALDI ToF/ToF (Matrix-Assisted Laser Desorption/Ionization Time of Flight) mass spectrometry techniques. Our investigation revealed that the main components of analysed venom are 3FTxs (Three-Finger Toxins) and PLA2s (Phospholipases A2). Additionally the presence of cysteine-rich venom proteins, 5′-nucleotidase and metalloproteinases has also been confirmed. The most interesting fact derived from this study is that the venom of N. ashei includes proteins not described previously in other African spitting cobras—cobra venom factor and venom nerve growth factor. To our knowledge, there are currently no other reports concerning this venom composition and we believe that our results will significantly increase interest in research of this species. Full article
(This article belongs to the Special Issue Analysis of Peptides and Proteins by Electrophoretic Techniques)

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