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Special Issue "Chemoselective Ligations"

A special issue of Molecules (ISSN 1420-3049). This special issue belongs to the section "Bioorganic Chemistry".

Deadline for manuscript submissions: 30 June 2018

Special Issue Editors

Guest Editor
Prof. Olivier Renaudet

Department of Molecular Chemistry, University Grenoble-Alpes
Website | E-Mail
Interests: carbohydrates; peptides; multivalency; chemical ligations; glycoconjugates; synthetic vaccines; antiadhesive agents; lectins; microarrays
Guest Editor
Dr. Nicolas Spinelli

Department of Molecular Chemistry, University Grenoble-Alpes
E-Mail
Interests: oligonucleotide; oxime; CuAAC; peptides; aptamers; G-quadruplexes; biosensors

Special Issue Information

Dear Colleagues,

Chemoselective ligations are undoubtedly the methods of choice for the synthesis of well-defined and complex biomacromolecular systems, based on peptides, carbohydrates, and/or oligonucleotides. This Special Issue will be dedicated to recent advances in this field, and will also have a special focus on biological applications of the resulting structures.

Olivier Renaudet
Nicolas Spinelli
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All papers will be peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Molecules is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 1800 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • carbohydrates
  • peptides
  • oligonucleotides
  • supramolecular chemistry
  • chemical ligation
  • bioorthogonality
  • click-chemistry

Published Papers (1 paper)

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Research

Open AccessArticle Immobilization of Staphylococcus aureus Sortase A on Chitosan Particles and Its Applications in Peptide-to-Peptide Ligation and Peptide Cyclization
Molecules 2018, 23(1), 192; https://doi.org/10.3390/molecules23010192
Received: 21 December 2017 / Revised: 11 January 2018 / Accepted: 14 January 2018 / Published: 19 January 2018
PDF Full-text (17160 KB) | HTML Full-text | XML Full-text | Supplementary Files
Abstract
Chitosan macro-particles prepared by the neutralization method were applied to Sortase A (SrtA) immobilization using glutaraldehyde as a crosslinking agent. The particles were characterized by Fourier transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM). Response surface methodology (RSM) was employed to optimize
[...] Read more.
Chitosan macro-particles prepared by the neutralization method were applied to Sortase A (SrtA) immobilization using glutaraldehyde as a crosslinking agent. The particles were characterized by Fourier transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM). Response surface methodology (RSM) was employed to optimize the immobilization process. An average specific activity of 3142 U (mg protein)−1 was obtained under optimized immobilization conditions (chitosan concentration 3%, SrtA concentration 0.5 mg·mL−1, glutaraldehyde concentration 0.5%, crosslinking and immobilization at 20 °C, crosslinking for 3 h, and an immobilization time of 8 h). The transpeptidase activity of immobilized SrtA was proved by a peptide-to-peptide ligation with a conversion yield approximately at 80%, and the immobilized catalyst was successfully reused for five cycles without obvious activity loss. Moreover, the scale-up capability of using immobilized SrtA to catalyze a head-to-tail peptide cyclization was investigated in a batch reaction and the conversion yield was more than 95% when using 20 mg of peptide as a substrate. Full article
(This article belongs to the Special Issue Chemoselective Ligations)
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