Special Issue "New and Old Technologies for Generation of Microarrays"
A special issue of Microarrays (ISSN 2076-3905).
Deadline for manuscript submissions: 31 January 2015
Dr. Günter Roth
Albert-Ludwigs-University Freiburg, Center for Biological Systems Analysis (ZBSA), Habsburgerstrasse 49, D-79104 Freiburg, Germany
Interests: microarrays; label-free detection and binding kinetic determination; mobile applications; copying NGS chips into microarrays
Microarrays have been the first method of choice for highly parallel DNA and RNA analysis as well as in molecular interaction studies. Even though Next Generation Sequencing (NGS) is believed to soon be able to count each RNA copy in a single cell; microarrays are still irreplaceable, especially in the field of proteins, antibodies and small molecules. Nevertheless the increasing demand in throughput, molecular purity, robustness and effective production leads to improvement of the old techniques of microarray generation and to innovative ideas of ‘making’ microarrays in completely new ways. Therefore ‘the simple act’ of Microarray Generation is the focus of this Special Issue “Microarray Generation—Old Paths and New Ways”. It will highlight the old and successful paths such as light-synthesis, spot-synthesis and outline future improvements to the reader, but also offer a glimpse into what is emerging at the moment and what is yet to come, i.e., what new possibilities may become available in the not too distant future. You are invited to present new ways of microarray generation, but also to provide proof that the old ‘paths’ still bear potential for vast improvements.
With best regards,
Dr. Günter Roth
Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. Papers will be published continuously (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.
Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are refereed through a peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Microarrays is an international peer-reviewed Open Access quarterly journal published by MDPI.
Please visit the Instructions for Authors page before submitting a manuscript. For the first couple of issues the Article Processing Charge (APC) will be waived for well-prepared manuscripts. English correction and/or formatting fees of 250 CHF (Swiss Francs) will be charged in certain cases for those articles accepted for publication that require extensive additional formatting and/or English corrections.
Special Issue Flyer
Please download the special issue flyer here.
- microarray generation
- DNA synthesis
- RNA synthesis
- protein synthesis
- microarray copying
- light synthesis
- spot synthesis
- microarray printing
The below list represents only planned manuscripts. Some of these manuscripts have not been received by the Editorial Office yet. Papers submitted to MDPI journals are subject to peer-review.
Title: Particle-based microarrays of oligonucleotides and oligopeptides
Authors: Alexander Nesterov-Mueller 1,*, Frieder Märkle 1, Tobias Förtsch 1, Sebastian Schillo 1, Lothar Hahn 1, Valentina Bykovskaya 1, Martyna Sedlmayr 1, Miriam Soehindrijo 1, Bastian Münster 1, Jakob Striffler 1, F. Ralf Bischoff 2, F. Breitling 1, Felix F. Loeffler 1,*
Affiliation: 1. Karlsruhe Institute of Technology, Hermann-von-Helmholtz-Platz 1, 76344 Eggenstein-Leopoldshafen, Germany; 2. German Cancer Research Center, Im Neuenheimer Feld 280, 69120 Heidelberg, Germany
Abstract: In this review we describe different methods of microarray fabrication based on the use of microparticles/-beads and point out future tendencies in the development of particle-based arrays. First, we consider oligonucleotide bead arrays, where each bead is a carrier of one specific sequence of oligonucleotides. This bead-based array approach, appearing in the late 1990s, enabled high-throughput oligonucleotide analysis and had a large impact on genome research. Furthermore, we consider particle-based peptide array fabrication using combinatorial chemistry. In this approach, particles can directly participate in both, the synthesis and the transfer of synthesized combinatorial molecules to a substrate. Subsequently, we describe in more detail the synthesis of peptide arrays with amino acid polymer particles, which imbed the amino acids inside their polymer matrix. By heating these particles, the polymer matrix is transformed into a highly viscous gel and, thereby, imbedded monomers are allowed to participate in the coupling reaction. Finally, we focus on combinatorial laser fusing of particles for the synthesis of high-density peptide arrays. This method combines the advantages of particles and combinatorial lithographic approaches.
Last update: 11 July 2014