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Microarrays 2015, 4(2), 214-227; doi:10.3390/microarrays4020214

NAPPA as a Real New Method for Protein Microarray Generation

1
Department of Medicine and General Cytometry Service-Nucleus, Cancer Research Centre (IBMCC/CSIC/USAL/IBSAL), Salamanca 37007, Spain
2
Proteomics Unit, Cancer Research Centre (IBMCC/CSIC/USAL/IBSAL), Salamanca 37007, Spain
3
Rheumatology Division, ProteoRed/ISCIII Proteomics Group, INIBIC, Hospital Universitario de A Coruña, A Coruña 15006, Spain
4
Biotechnology National Centre, Spanish National Research Council (CSIC), Madrid 28049, Spain
5
Biodesign Institute, Arizona State University, 1001 South McAllister Avenue, Tempe, AZ 85287, USA
*
Author to whom correspondence should be addressed.
Academic Editor: Ulrich Certa
Received: 5 March 2015 / Revised: 30 March 2015 / Accepted: 14 April 2015 / Published: 24 April 2015
(This article belongs to the Special Issue New and Old Technologies for Generation of Microarrays)
View Full-Text   |   Download PDF [1061 KB, uploaded 24 April 2015]   |  

Abstract

Nucleic Acid Programmable Protein Arrays (NAPPA) have emerged as a powerful and innovative technology for the screening of biomarkers and the study of protein-protein interactions, among others possible applications. The principal advantages are the high specificity and sensitivity that this platform offers. Moreover, compared to conventional protein microarrays, NAPPA technology avoids the necessity of protein purification, which is expensive and time-consuming, by substituting expression in situ with an in vitro transcription/translation kit. In summary, NAPPA arrays have been broadly employed in different studies improving knowledge about diseases and responses to treatments. Here, we review the principal advances and applications performed using this platform during the last years. View Full-Text
Keywords: protein microarray; NAPPA; high-throughput screening; biomarker; protein-protein interaction; microarray generation protein microarray; NAPPA; high-throughput screening; biomarker; protein-protein interaction; microarray generation
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Díez, P.; González-González, M.; Lourido, L.; Dégano, R.M.; Ibarrola, N.; Casado-Vela, J.; LaBaer, J.; Fuentes, M. NAPPA as a Real New Method for Protein Microarray Generation. Microarrays 2015, 4, 214-227.

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