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From the third issue of 2017, Microarrays has changed its name to High-Throughput.

Open AccessReview
Microarrays 2014, 3(4), 245-262; doi:10.3390/microarrays3040245

Particle-Based Microarrays of Oligonucleotides and Oligopeptides

1
Karlsruhe Institute of Technology, Hermann-von-Helmholtz-Platz 1, 76344 Eggenstein-Leopoldshafen, Germany
2
German Cancer Research Center, Im Neuenheimer Feld 280, 69120 Heidelberg, Germany
*
Authors to whom correspondence should be addressed.
Received: 4 August 2014 / Revised: 15 October 2014 / Accepted: 16 October 2014 / Published: 28 October 2014
(This article belongs to the Special Issue New and Old Technologies for Generation of Microarrays)
View Full-Text   |   Download PDF [1324 KB, uploaded 28 October 2014]   |  

Abstract

In this review, we describe different methods of microarray fabrication based on the use of micro-particles/-beads and point out future tendencies in the development of particle-based arrays. First, we consider oligonucleotide bead arrays, where each bead is a carrier of one specific sequence of oligonucleotides. This bead-based array approach, appearing in the late 1990s, enabled high-throughput oligonucleotide analysis and had a large impact on genome research. Furthermore, we consider particle-based peptide array fabrication using combinatorial chemistry. In this approach, particles can directly participate in both the synthesis and the transfer of synthesized combinatorial molecules to a substrate. Subsequently, we describe in more detail the synthesis of peptide arrays with amino acid polymer particles, which imbed the amino acids inside their polymer matrix. By heating these particles, the polymer matrix is transformed into a highly viscous gel, and thereby, imbedded monomers are allowed to participate in the coupling reaction. Finally, we focus on combinatorial laser fusing of particles for the synthesis of high-density peptide arrays. This method combines the advantages of particles and combinatorial lithographic approaches. View Full-Text
Keywords: high-throughput; combinatorial synthesis; beads high-throughput; combinatorial synthesis; beads
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Nesterov-Mueller, A.; Maerkle, F.; Hahn, L.; Foertsch, T.; Schillo, S.; Bykovskaya, V.; Sedlmayr, M.; Weber, L.K.; Ridder, B.; Soehindrijo, M.; Muenster, B.; Striffler, J.; Bischoff, F.R.; Breitling, F.; Loeffler, F.F. Particle-Based Microarrays of Oligonucleotides and Oligopeptides. Microarrays 2014, 3, 245-262.

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