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Dietary Effects on Gut Microbial Metabolism and Intestinal Inflammation in Mammals

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A special issue of Metabolites (ISSN 2218-1989). This special issue belongs to the section "Microbiology and Ecological Metabolomics".

Deadline for manuscript submissions: closed (15 August 2022) | Viewed by 9957

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Special Issue Editors

Dr. Haoyu Liu

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Guest Editor

Department of Medical Cell Biology, Research Group Lena Holm, Uppsala University, Uppsala, Sweden
Interests: dietary intervention; inflammation; mucosal immunology; nutrigenetics

Dr. Bei Gao

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Guest Editor

School of Marine Sciences, Nanjing University of Information Science and Technology, Nanjing, China
Interests: metabolomics; lipidomics; stable isotope tracing; gut microbiota; multi-omics; environmental toxicology; alcohol-related liver disease
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Special Issue Information

Dear Colleagues,

Gut microbial metabolites tremendously impact the health of their host, such as short-chain fatty acids, polyamines, polyphenols, bile acids, trimethylamine N-oxide, and vitamins. Metabolomics is an essential perspective through which interactions among diet, gut microbiota, and intestinal inflammation in mammals can be explored. Given that gut microbial metabolism plays an significant role in mammalian health, this Special Issue of Metabolites is dedicated to publishing state-of-the-art research that reveals the dietary effects of gut microbial metabolites on gut microbial metabolism and intestinal inflammation using untargeted and targeted metabolomics and lipidomics approaches; nutrigenomics; tracing the fate of gut microbial metabolites using stable isotope tracers; identifying novel gut-microbiota-derived metabolites and lipids using cheminformatics; and unravelling microbial enzymes and key genes involved in the conversion of metabolites and lipids by integrating metabolomics and lipidomics with other -omics.

Dr. Haoyu Liu
Dr. Bei Gao
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Metabolites is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

metabolomics
lipidomics
stable isotope tracing
gut microbiota
multi-omics
environmental toxicology
alcohol-related liver disease

Published Papers (6 papers)

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Editorial

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2 pages, 163 KiB

Open AccessEditorial

Dietary Effects on Gut Microbial Metabolism and Intestinal Inflammation in Mammals

by Bei Gao and Haoyu Liu

Metabolites 2023, 13(9), 1025; https://doi.org/10.3390/metabo13091025 - 20 Sep 2023

Viewed by 696

Abstract

Growing evidence has proven that the gut microbiota has a tremendous impact on mammalian health [...] Full article

(This article belongs to the Special Issue Dietary Effects on Gut Microbial Metabolism and Intestinal Inflammation in Mammals)

Research

Jump to: Editorial

13 pages, 3222 KiB

Open AccessArticle

Characterizing the Gut Microbial Metabolic Profile of Mice with the Administration of Berry-Derived Cyanidin-3-Glucoside

by Pengcheng Tu, Xiaodong Zheng, Huixia Niu, Zhijian Chen, Xiaofeng Wang, Lizhi Wu and Qiong Tang

Metabolites 2023, 13(7), 818; https://doi.org/10.3390/metabo13070818 - 4 Jul 2023

Cited by 1 | Viewed by 790

Abstract

Dietary modulation of the gut microbiota has recently received considerable attention. It is well established that consumption of berries confers a number of health benefits. We previously reported that a black raspberry (BRB)-rich diet effectively modulates the gut microbiota. Given the role of anthocyanins in the health benefits of berries, coupled with interactions of gut microbial metabolites with host health, the objective of this follow-up study was to further characterize the profile of functional metabolites in the gut microbiome modulated by anthocyanins. We utilized a berry-derived classic anthocyanin, cyanidin-3-glucoside (C3G), combined with a mouse model to probe C3G-associated functional metabolic products of gut bacteria through a mass spectrometry-based metabolomic profiling technique. Results showed that C3G substantially changed the gut microbiota of mice, including its composition and metabolic profile. A distinct metabolic profile in addition to a variety of key microbiota-related metabolites was observed in C3G-treated mice. Microbial metabolites involved in protein digestion and absorption were differently abundant between C3G-treated and control mice, which may be linked to the effects of berry consumption. Results of the present study suggest the involvement of the gut microbiota in the health benefits of C3G, providing evidence connecting the gut microbiota with berry consumption and its beneficial effects. Full article

(This article belongs to the Special Issue Dietary Effects on Gut Microbial Metabolism and Intestinal Inflammation in Mammals)

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13 pages, 2178 KiB

Open AccessArticle

Dietary Administration of Black Raspberries and Arsenic Exposure: Changes in the Gut Microbiota and Its Functional Metabolites

by Pengcheng Tu, Qiong Tang, Zhe Mo, Huixia Niu, Yang Hu, Lizhi Wu, Zhijian Chen, Xiaofeng Wang and Bei Gao

Metabolites 2023, 13(2), 207; https://doi.org/10.3390/metabo13020207 - 30 Jan 2023

Cited by 2 | Viewed by 1096

Abstract

Mounting evidence has linked berries to a variety of health benefits. We previously reported that administration of a diet rich in black raspberries (BRBs) impacted arsenic (As) biotransformation and reduced As-induced oxidative stress. To further characterize the role of the gut microbiota in BRB-mediated As toxicity, we utilized the dietary intervention of BRBs combined with a mouse model to demonstrate microbial changes by examining associated alterations in the gut microbiota, especially its functional metabolites. Results showed that BRB consumption changed As-induced gut microbial alterations through restoring and modifying the gut microbiome, including its composition, functions and metabolites. A number of functional metabolites in addition to bacterial genera were significantly altered, which may be linked to the effects of BRBs on arsenic exposure. Results of the present study suggest functional interactions between dietary administration of black raspberries and As exposure through the lens of the gut microbiota, and modulation of the gut microbiota and its functional metabolites could contribute to effects of administration of BRBs on As toxicity. Full article

(This article belongs to the Special Issue Dietary Effects on Gut Microbial Metabolism and Intestinal Inflammation in Mammals)

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15 pages, 2939 KiB

Open AccessEditor’s ChoiceArticle

Effects of Proteases from Pineapple and Papaya on Protein Digestive Capacity and Gut Microbiota in Healthy C57BL/6 Mice and Dose-Manner Response on Mucosal Permeability in Human Reconstructed Intestinal 3D Tissue Model

by Olha Kostiuchenko, Nadiia Kravchenko, Jan Markus, Stephen Burleigh, Olexandr Fedkiv, Ling Cao, Silvia Letasiova, Galyna Skibo, Frida Fåk Hållenius and Olena Prykhodko

Metabolites 2022, 12(11), 1027; https://doi.org/10.3390/metabo12111027 - 26 Oct 2022

Cited by 7 | Viewed by 2750

Abstract

Cysteine proteases obtained from the stem of pineapple or papaya latex, bromelain and papain, respectively, exhibit a broad spectrum of beneficial effects on human health. However, their effects on gut microbiota composition or dose-manner effects on the intestinal integrity of healthy tissue have not been evaluated. In this study, C57BL/6 young, healthy mice were fed bromelain or papain in a dose of 1 mg per animal/day for three consecutive days, followed by the assessment of digestive protein capacity, intestinal morphology and gut microbiota composition. Furthermore, a human reconstructed 3D tissue model EpiIntestinal (SMI-100) was used to study the effects of 1, 0.1 and 10 mg/mL doses of each enzyme on tissue integrity and mucosal permeability using TEER measurements and passage of Lucifer Yellow marker from the apical to the basolateral side of the mucosa. The results indicated that fruit proteases have the potential to modulate gut microbiota with decreasing abundance of Proteobacteria and increasing beneficial Akkermansia muciniphila. The enhancement of pancreatic trypsin was observed in bromelain and papain supplementation, while bromelain also increased the thickness of the ileal mucosa. Furthermore, an in vitro study showed a dose-dependent interruption in epithelial integrity, which resulted in increased paracellular permeability by the highest doses of enzymes. These findings define bromelain and papain as promising enzymatic supplementation for controlled enhancement of paracellular uptake when needed, together with beneficial effects on the gut microbiota. Full article

(This article belongs to the Special Issue Dietary Effects on Gut Microbial Metabolism and Intestinal Inflammation in Mammals)

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14 pages, 2025 KiB

Open AccessArticle

Geranylgeraniol and Green Tea Polyphenols Mitigate Negative Effects of a High-Fat Diet on Skeletal Muscle and the Gut Microbiome in Male C57BL/6J Mice

by Chwan-Li Shen, Moamen M. Elmassry, Katherine Grue, Hayli E. Joiner, A. Unique Jacobo, Abdul Hamood and Eunhee Chung

Metabolites 2022, 12(10), 913; https://doi.org/10.3390/metabo12100913 - 27 Sep 2022

Cited by 2 | Viewed by 1786

Abstract

Natural bioactive compounds are proposed as alternatives in mitigating obesity-associated skeletal muscle dysfunction. The objective of this study was to test the hypothesis that the combination of geranylgeraniol (GGOH) and green tea polyphenols (GTPs) can alleviate high-fat-diet (HFD)-induced muscle atrophy and alter gut microbiome composition. Male C57BL/6J mice fed an HFD were assigned to four groups (12 mice each) in a 2 (no GGOH vs. 400 mg GGOH/kg diet) × 2 (no GTPs vs. 0.5% weight/volume GTPs in water) factorial design. After 14 weeks of diet intervention, skeletal muscle and cecal samples were collected and examined. Compared to the control groups, the group that consumed a combination of GGOH and GTPs (GG + GTPs) had significantly decreased body and fat mass but increased skeletal muscle mass normalized by body weight and cross-sectional area. In soleus muscle, the GG + GTP diet increased citrate synthase activity but decreased lipid peroxidation. Gut microbiome beta-diversity analysis revealed a significant difference in the microbiome composition between diet groups. At the species level, the GG + GTP diet decreased the relative abundance of Dorea longicatena, Sporobacter termitidis, and Clostridium methylpentosum, and increased that of Akkermansia muciniphila and Subdoligranulum variabile. These results suggest that the addition of GGOH and GTPs to an HFD alleviates skeletal muscle atrophy, which is associated with changes in the gut microbiome composition. Full article

(This article belongs to the Special Issue Dietary Effects on Gut Microbial Metabolism and Intestinal Inflammation in Mammals)

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11 pages, 2420 KiB

Open AccessArticle

Lithocholic Acid Alleviates Deoxynivalenol-Induced Lethal Cholesterol Metabolic Abnormalities in IPI-2I Cells

by Yanwei Li, Fang Gu, Haotian Gu, Ping Hu, Hui-Xin Liu and Demin Cai

Metabolites 2022, 12(7), 659; https://doi.org/10.3390/metabo12070659 - 17 Jul 2022

Cited by 4 | Viewed by 1886

Abstract

Deoxynivalenol (DON) is a secondary metabolite of fungi. Ingestion of feed containing DON causes severe intestinal damage in humans and animals, possibly due to cholesterol-enriched lipid raft abnormalities. Cholic acid (CA) and lithocholic acid (LCA) are metabolites of cholesterol transformation, which have been proven to benefit epithelial cell proliferation and reduce intestinal inflammation and lesions. Therefore, we aimed to study the protective roles of CA and LCA administration on the DON-exposed intestinal epithelial cells (IPI-2I) and the underlying mechanisms involved in cholesterol metabolism. We found that LCA pretreatment, but not CA, alleviated the reduction of cell numbers caused by DON exposure. Furthermore, we demonstrate that LCA restored the DON-induced cell apoptosis by reducing the cleaved caspase 3 and cleaved PARP-1 expression. DON-increased cellular cholesterol and bile acid contents were significantly reduced when LCA was co-treated. Further transcriptomic analysis revealed that the aberrant cholesterol homeostasis genes profile was observed in the cells exposed to DON or pretreated with LCA. We also validated that the key genes involved in cholesterol biosynthesis and transformation (cholesterol to bile acids) were strongly inhibited by the LCA treatment in the DON-exposed cells. Together, this study demonstrated that LCA ameliorated DON-caused toxic apoptosis in IPI-2I cells by maintaining cholesterol metabolism. We suggest that as an endogenous metabolite, LCA may be used as a therapeutic and/or integrated into a dietary intervention against mycotoxin toxicity. Full article

(This article belongs to the Special Issue Dietary Effects on Gut Microbial Metabolism and Intestinal Inflammation in Mammals)

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