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Animals
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Frozen Semen in Ruminants
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Frozen Semen in Ruminants

A special issue of Animals (ISSN 2076-2615). This special issue belongs to the section "Animal Reproduction".

Deadline for manuscript submissions: closed (31 October 2023) | Viewed by 2623

Special Issue Editor

Prof. Dr. Lingjiang Min

E-Mail Website
Guest Editor
College of Animal Science and Technology, Qingdao Agricultural University, Qingdao, China
Interests: semen; ruminant genetics; sperm

Special Issue Information

Dear Colleagues,

Sperm cryopreservation is one of the most important biotechnologies in ruminants’ artificial insemination. Although cryopreserved procedures and freezing medium in ruminants’ sperm cryopreservation process have been developed in the past few decades, the ruminant’s frozen sperm quality is still much lower than those in fresh or liquid storage. In addition, there is no standardized methodology for each of the stages of the cryopreservation procedure in ruminants’ semen cryopreservation, such as in goat and sheep species. Moreover, previous studies show that sperm cryotolerance is not only dependent on species but also related to the individual. Some cryotolerance markers of sperm and seminal plasma have been evaluated for prediction of ejaculate sperm freezability, but the mechanism is still unclear. Therefore, the aim of the current research topic is to focus on the cryopreserved procedures or the freezing medium components (antioxidants, cryoprotectants, etc.) that can help to improve the quality of frozen semen in ruminants.

We invite original research/review papers that address improvements in the quality of frozen semen in ruminants. Areas of interest include supplementation of freezing media with additives (such as antioxidants, amino acids, cryoprotectants, etc.) or the development of techniques or biotechnology for cryopreservation in ruminants.

Prof. Dr. Lingjiang Min
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Animals is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2400 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • cryopreservation
  • sperm
  • frozen
  • quality of frozen semen

Published Papers (2 papers)

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Research

13 pages, 1614 KiB  
Article
Grape Seed Proanthocyanidins Improve the Quality of Fresh and Cryopreserved Semen in Bulls
by Meng Wang, Silin Wu, Benshun Yang, Miaomiao Ye, Jianbing Tan, Linsen Zan and Wucai Yang
Animals 2023, 13(17), 2781; https://doi.org/10.3390/ani13172781 - 31 Aug 2023
Viewed by 1242
Abstract
Oxidative stress leads to a decrease in semen quality during semen cryopreservation and fresh semen production. Grape seed proanthocyanidins (GSPs) are endowed with well-recognized antioxidant, anti-inflammatory, anti-cancer, and anti-aging activities. Therefore, the objective of this experiment was to explore the effects of GSPs [...] Read more.
Oxidative stress leads to a decrease in semen quality during semen cryopreservation and fresh semen production. Grape seed proanthocyanidins (GSPs) are endowed with well-recognized antioxidant, anti-inflammatory, anti-cancer, and anti-aging activities. Therefore, the objective of this experiment was to explore the effects of GSPs on the quality of fresh and cryopreserved semen to provide a basis for GSPs as a new dietary additive and semen diluent additive for males’ reproduction. Fresh semen from three healthy bulls aged 3 to 5 years old were gathered and mixed with semen diluents dissolved with 0 µg/mL, 30 µg/mL, 40 µg/mL, 50 µg/mL, and 60 µg/mL GSPs respectively. The motility, physiological structures (acrosome integrity, membrane integrity, mitochondrial activity), and antioxidant capacity of frozen–thawed sperm were measured after storage in liquid nitrogen for 7 days (d). Bulls were fed with 20 mg/kg body weight (BW) GSPs in their diet for 60 days; the weight of the bull is about 600 kg. Then, the reproductive performance and antioxidant indexes of bulls were measured before and after feeding. The results demonstrated that GSPs supplementation significantly increased sperm motility, physiological structures, GSH-Px, and CAT enzyme activities and significantly decreased MDA content in sperm during semen cryopreservation. The optimal concentration of GSPs was 40 µg/mL (p < 0.05). After 20 mg/kg (body weight) GSP supplementation, sperm motility was significantly heightened (p < 0.05), the sperm deformity rate was significantly reduced (p < 0.05), and antioxidant enzyme activities (such as SOD, CAT, and GSH-Px) were significantly enhanced (p < 0.05), and the production of MDA was significantly suppressed (p < 0.05) in serum compared with that before feeding. In conclusion, these results reveal that a certain concentration of GSPs has a good protective effect on sperm damage caused by semen cryopreservation and the reproductive performance reduction caused by stress in bulls, which may be attributed to the antioxidant function of GSPs. In summary, GSPs are a useful cryoprotective adjuvant and dietary additive for bull sperm quality. Full article
(This article belongs to the Special Issue Frozen Semen in Ruminants)
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15 pages, 2537 KiB  
Article
The Beneficial Effects of Pterostilbene on Post-Thawed Bovine Spermatozoa
by Vasiliki Sapanidou, Maria Tsantarliotou, Sophia Lavrentiadou, Elena Tzekaki, Ioannis Efraimidis, Theodoros Lialiaris and Byron Asimakopoulos
Animals 2023, 13(17), 2713; https://doi.org/10.3390/ani13172713 - 25 Aug 2023
Viewed by 904
Abstract
Reactive Oxygen Species (ROS), primarily produced by cellular metabolism, are highly reactive molecules that modify cellular compounds. During sperm preparation in Assisted Reproductive Techniques (ARTs), intrinsic and extrinsic sources of ROS can impact spermatozoa’s oxidative status. The modification of the media with compounds [...] Read more.
Reactive Oxygen Species (ROS), primarily produced by cellular metabolism, are highly reactive molecules that modify cellular compounds. During sperm preparation in Assisted Reproductive Techniques (ARTs), intrinsic and extrinsic sources of ROS can impact spermatozoa’s oxidative status. The modification of the media with compounds that enhance sperm quality characteristics is of great significance. The current study investigated the effect of pterostilbene, a phenolic compound, on bovine sperm quality. Cryopreserved spermatozoa from six bulls were thawed, supplemented with pterostilbene (0, 10 μΜ, 25 μΜ) and incubated for 60 min and 240 min. Spermatozoa were analyzed in terms of motility, viability, acrosomal status and intracellular concentration of superoxide anion in each time point. The incubation of spermatozoa with 25 μΜ pterostilbene resulted in the preservation of quality parameters through superoxide anion mitigation, while its presence in capacitating conditions resulted in higher percentage of acrosome-reacted spermatozoa. The results of the present study indicate that the addition of pterostilbene prevents oxidative insult to spermatozoa and preserves the sperm quality parameters. Full article
(This article belongs to the Special Issue Frozen Semen in Ruminants)
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