Special Issue "Recent Advancements in Analysis of Biomolecules with Separation Technologies"


A special issue of Separations (ISSN 2297-8739).

Deadline for manuscript submissions: closed (30 June 2014)

Special Issue Editor

Guest Editor
Dr. Wenwan Zhong

Department of Chemistry, University of California, Riverside, CA 92508, USA
Website: http://faculty.ucr.edu/~wenwanz
Interests: capillary electrophoresis; flow field flow fractionation; molecular interaction; biosening; nano-bio interface

Special Issue Information

Dear Colleagues,

Separation technologies have advanced significantly in the past decade. In particular, development of new techniques as well as application/improvement of existing techniques for analysis of small compounds important for biological processes, macromolecules like nucleic acids, proteins, carbohydrates, and lipids, and bioparticles such as cell organelles, viruses, and bacteria, have provided great assistance to the exploration of systems biology.  Therefore, this special issue will be devoted to the new development, technology advancement, and novel application of separation technology for study of biomolecules and/or their functions. With this issue, we hope to highlight the significant contribution of separation science towards understanding how biosystems function, and to illuminate the future direction of bioseparation for imposing higher impacts to our society.

Dr. Wenwan Zhong
Guest Editor


Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. Papers will be published continuously (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are refereed through a peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Separations is an international peer-reviewed Open Access quarterly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. For the first couple of issues the Article Processing Charge (APC) will be waived for well-prepared manuscripts. English correction and/or formatting fees of 250 CHF (Swiss Francs) will be charged in certain cases for those articles accepted for publication that require extensive additional formatting and/or English corrections.


  • separation
  • biologically relevant molecules
  • analysis

Published Papers (4 papers)

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Displaying article 1-4
p. 176-193
by , , ,  and
Chromatography 2014, 1(4), 176-193; doi:10.3390/chromatography1040176
Received: 1 July 2014 / Revised: 11 September 2014 / Accepted: 18 September 2014 / Published: 13 October 2014
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p. 159-175
by , , , , , ,  and
Chromatography 2014, 1(4), 159-175; doi:10.3390/chromatography1040159
Received: 1 July 2014 / Revised: 3 September 2014 / Accepted: 11 September 2014 / Published: 9 October 2014
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p. 108-119
by ,  and
Chromatography 2014, 1(3), 108-119; doi:10.3390/chromatography1030108
Received: 8 May 2014 / Revised: 20 June 2014 / Accepted: 24 June 2014 / Published: 2 July 2014
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p. 55-64
by  and
Chromatography 2014, 1(2), 55-64; doi:10.3390/chromatography1020055
Received: 27 January 2014 / Revised: 10 March 2014 / Accepted: 17 March 2014 / Published: 26 March 2014
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Planned Papers

The below list represents only planned manuscripts. Some of these manuscripts have not been received by the Editorial Office yet. Papers submitted to MDPI journals are subject to peer-review.

Title: Analytical and preparative applications of mixed-mode chromatography
Tsutomu Arakawa, Daisuke Ejima, Mark Feild, Janice Davis
Alliance Protein Laboratories; Ajinomoto Co. Inc.; Ajinomoto Althea, Inc.
This review covers our experience with mixed-mode chromatography for analysis and purification of proteins. Mixed-mode resins are composed of multiple protein binding sites, thereby providing different binding mechanisms that do not occur in such single-mode resins as ion-exchange and hydrophobic interaction chromatography. Commonly-used mixed-mode resins contain ionic and hydrophobic groups and hence can bind protein by electrostatic and hydrophobic interactions. While such multiple binding mechanisms render protein binding stronger, they do often complicate elution of the bound protein, as it may require disrupting both electrostatic and hydrophobic interactions simultaneously. We will review binding and elution properties of mixed-mode chromatography using a few model proteins. A novel finding in application of mixed-mode chromatography is resolution of isoforms of a same protein, which will also be discussed.
mixed-mode chromatography, electrostatic, hydrophobic, isoform, elution, arginine.

Last update: 5 March 2014

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