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From the first issue of 2016, Chromatography has changed its name to Separations.

Open AccessTechnical Note
Chromatography 2014, 1(4), 159-175; doi:10.3390/chromatography1040159

High-Throughput Mass Spectrometry Applied to Structural Genomics

Structural Genomics Consortium, University of Oxford, Old Road Campus Research Building, Roosevelt Drive, Oxford OX3 7DQ, UK
These authors contributed equally to this work.
Author to whom correspondence should be addressed.
Received: 1 July 2014 / Revised: 3 September 2014 / Accepted: 11 September 2014 / Published: 9 October 2014
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Mass spectrometry (MS) remains under-utilized for the analysis of expressed proteins because it is inaccessible to the non-specialist, and sample-turnaround from service labs is slow. Here, we describe 3.5 min Liquid-Chromatography (LC)-MS and 16 min LC-MSMS methods which are tailored to validation and characterization of recombinant proteins in a high throughput structural biology pipeline. We illustrate the type and scope of MS data typically obtained from a 96-well expression and purification test for both soluble and integral membrane proteins (IMPs), and describe their utility in the selection of constructs for scale-up structural work, leading to cost and efficiency savings. We propose that value of MS data lies in how quickly it becomes available and that this can fundamentally change the way in which it is used. View Full-Text
Keywords: high-throughput; mass spectrometry; protein expression; structural genomics; integral membrane protein (IMP); post-translational modification (PTM); intact mass high-throughput; mass spectrometry; protein expression; structural genomics; integral membrane protein (IMP); post-translational modification (PTM); intact mass

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Chalk, R.; Berridge, G.; Shrestha, L.; Strain-Damerell, C.; Mahajan, P.; Yue, W.W.; Gileadi, O.; Burgess-Brown, N.A. High-Throughput Mass Spectrometry Applied to Structural Genomics. Chromatography 2014, 1, 159-175.

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