High-Throughput Mass Spectrometry Applied to Structural Genomics
AbstractMass spectrometry (MS) remains under-utilized for the analysis of expressed proteins because it is inaccessible to the non-specialist, and sample-turnaround from service labs is slow. Here, we describe 3.5 min Liquid-Chromatography (LC)-MS and 16 min LC-MSMS methods which are tailored to validation and characterization of recombinant proteins in a high throughput structural biology pipeline. We illustrate the type and scope of MS data typically obtained from a 96-well expression and purification test for both soluble and integral membrane proteins (IMPs), and describe their utility in the selection of constructs for scale-up structural work, leading to cost and efficiency savings. We propose that value of MS data lies in how quickly it becomes available and that this can fundamentally change the way in which it is used. View Full-Text
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Chalk, R.; Berridge, G.; Shrestha, L.; Strain-Damerell, C.; Mahajan, P.; Yue, W.W.; Gileadi, O.; Burgess-Brown, N.A. High-Throughput Mass Spectrometry Applied to Structural Genomics. Chromatography 2014, 1, 159-175.
Chalk R, Berridge G, Shrestha L, Strain-Damerell C, Mahajan P, Yue WW, Gileadi O, Burgess-Brown NA. High-Throughput Mass Spectrometry Applied to Structural Genomics. Chromatography. 2014; 1(4):159-175.Chicago/Turabian Style
Chalk, Rod; Berridge, Georgina; Shrestha, Leela; Strain-Damerell, Claire; Mahajan, Pravin; Yue, Wyatt W.; Gileadi, Opher; Burgess-Brown, Nicola A. 2014. "High-Throughput Mass Spectrometry Applied to Structural Genomics." Chromatography 1, no. 4: 159-175.