Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
5.3
4.1
Journals
Metabolites
Special Issues
Intercellular Metabolome
share announcement

Intercellular Metabolome

A special issue of Metabolites (ISSN 2218-1989). This special issue belongs to the section "Cell Metabolism".

Deadline for manuscript submissions: closed (31 July 2020) | Viewed by 8618

Special Issue Editors

Dr. Ulrike E. Rolle-Kampczyk

E-Mail Website
Guest Editor
Helmholtz Zentrum für Umweltforschung, Department of Molecular Systems Biology, 15, 04318 Leipzig, Germany
Interests: metabolomics (targeted/untargeted); human biomonitoring; microbiom research; mass spectrometry
Special Issues, Collections and Topics in MDPI journals
Dr. Beatrice Engelmann

E-Mail Website
Guest Editor
Helmholtz Zentrum für Umweltforschung, 15, 04318 Leipzig, Germany
Interests: targeted and untargeted metabolomics; LC-MS/MS-based methodology development; biomarkers
Special Issues, Collections and Topics in MDPI journals
Dr. Sven-Bastiaan Haange

E-Mail Website
Guest Editor
Helmholtz Zentrum für Umweltforschung, 15, 04318 Leipzig, Germany
Interests: intestinal microbiome; multi-omics; metabolomics (targeted/untargeted); metaproteomics; 16S rRNA gene sequencing
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

The metabolome is influenced by endogenous and exogenous factors. Age, sex, health status, physical activity, diet, life style factors, and environmental noxae can affect the metabolome in the short as well as the long term. Exogenous and intercellular metabolites are some of the main facilitators of vital intercellular interactions. These interactions cover processes involved from nutrient distribution to signalling. Not only do these interactions take place within an organism but they can also occur between different organisms, such as between members of the intestinal microbiome or between the microbiome and the host. These facts highlight how crucial the analysis of exogenous and intercellular metabolites is.

With the rapid development of metabolomics techniques, it is possible to describe these processes more and more accurately and to help elucidate the underlying mechanisms. This Special Issue of Metabolites, "Exogenous/Intercellular Metabolome", will be dedicated to strategies dealing with these interactions. This Issue is not only intended for results from basic research (cell or animal models) but is also open to results from epidemiological studies. In addition, new measurement methods, bioinformatical tools, and data analysis concepts are welcome.

Dr. Ulrike E. Rolle-Kampczyk
Dr. Beatrice Engelmann
Dr. Sven-Bastiaan Haange
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Metabolites is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • Exogenous/intercellular metabolites
  • Targeted metabolomics
  • Untargeted metabolomics
  • Microbiome interactions
  • Cellular interactions
  • Method development
  • Bioinformatical tools

Published Papers (2 papers)

Download All Papers
Order results
Result details
Show export options expand_more Show export options expand_less
Select all
Export citation of selected articles as:

Research

15 pages, 2036 KiB  
Article
Multiplexed Quantitative Assessment of the Fate of Taurine and Sulfoquinovose in the Intestinal Microbiome
by Sven-Bastiaan Haange, Nicole Groeger, Jean Froment, Theresa Rausch, Wiebke Burkhardt, Svenja Gonnermann, Annett Braune, Michael Blaut, Martin von Bergen and Ulrike Rolle-Kampczyk
Metabolites 2020, 10(11), 430; https://doi.org/10.3390/metabo10110430 - 26 Oct 2020
Cited by 6 | Viewed by 2709
Abstract
(1) Introduction: Sulfonates, which can be diet- or host-derived, are a class of compounds detected in the gut, are involved in host–microbiome interactions and have several health effects. Our aim was to develop a method to quantify five of the sulfonates in the [...] Read more.
(1) Introduction: Sulfonates, which can be diet- or host-derived, are a class of compounds detected in the gut, are involved in host–microbiome interactions and have several health effects. Our aim was to develop a method to quantify five of the sulfonates in the intestine and apply it in a simplified human microbiome model. These were taurine, its metabolic precursor cysteate and one of its degradation products isethionate, as well as sulfoquinovose and one of its most relevant degradation products 2,3-dihydroxy-1-propanesulfonate. (2) Methods: An extraction and sample preparation method was developed, without the need for derivatization. To detect and quantify the extracted sulfonates, a multiplexed LC-MS/MS-MRM method was established. (3) Results: The accuracy and precision of the method were within GLP-accepted parameters. To apply this method in a pilot study, we spiked either taurine or sulfoquinovose into an in vitro simplified human microbiota model with and without Bilophila wadsworthia, a known sulfonate utilizer. The results revealed that only the culture with B. wadsworthia was able to degrade taurine, with isethionate as an intermediate. After spiking the communities with sulfoquinovose, the results revealed that the simplified human microbiome model was able to degrade sulfoquinovose to 2,3-dihydroxypropane-1-sulfonate, which was probably catalyzed by Escherichia coli. In the community with B. wadsworthia, the 2,3-dihydroxypropane-1-sulfonate produced was further degraded by B. wadsworthia to sulfide. (4) Conclusions: We successfully developed a method for sulfonate quantification and applied it in a first pilot study. Full article
(This article belongs to the Special Issue Intercellular Metabolome)
►▼ Show Figures

Graphical abstract

17 pages, 2156 KiB  
Article
Lipidomic Analysis of Cells and Extracellular Vesicles from High- and Low-Metastatic Triple-Negative Breast Cancer
by Nao Nishida-Aoki, Yoshihiro Izumi, Hiroaki Takeda, Masatomo Takahashi, Takahiro Ochiya and Takeshi Bamba
Metabolites 2020, 10(2), 67; https://doi.org/10.3390/metabo10020067 - 13 Feb 2020
Cited by 50 | Viewed by 5461
Abstract
Extracellular vesicles (EVs) are lipid bilayer nanovesicles secreted from almost all cells including cancer. Cancer-derived EVs contribute to cancer progression and malignancy via educating the surrounding normal cells. In breast cancer, epidemiological and experimental observations indicated that lipids are associated with cancer malignancy. [...] Read more.
Extracellular vesicles (EVs) are lipid bilayer nanovesicles secreted from almost all cells including cancer. Cancer-derived EVs contribute to cancer progression and malignancy via educating the surrounding normal cells. In breast cancer, epidemiological and experimental observations indicated that lipids are associated with cancer malignancy. However, lipid compositions of breast cancer EVs and their contributions to cancer progression are unexplored. In this study, we performed a widely targeted quantitative lipidomic analysis in cells and EVs derived from high- and low-metastatic triple-negative breast cancer cell lines, using supercritical fluid chromatography fast-scanning triple-quadrupole mass spectrometry. We demonstrated the differential lipid compositions between EVs and cells of their origin, and between high- and low-metastatic cell lines. Further, we demonstrated EVs from highly metastatic breast cancer accumulated unsaturated diacylglycerols (DGs) compared with EVs from lower-metastatic cells, without increasing the amount in cells. The EVs enriched with DGs could activate the protein kinase D signaling pathway in endothelial cells, which can lead to stimulated angiogenesis. Our results indicate that lipids are selectively loaded into breast cancer EVs to support tumor progression. Full article
(This article belongs to the Special Issue Intercellular Metabolome)
►▼ Show Figures

Figure 1

Show export options expand_more Show export options expand_less
Select all
Export citation of selected articles as:
 
Displaying articles 1-2
Back to TopTop