Special Issue "Protein Conjugates and Bioconjugate Chemistry"
Deadline for manuscript submissions: closed (29 February 2012)
Prof. Dr. Teruyuki Nagamune
Department of Bioengineering, School of Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan
The modification or conjugation of proteins, DNAs, and other biopolymers with functional molecules has become a very powerful research tool in biological science, biomedical research, and biotechnology. In particular, in the current post genomic era, much research requires chemically modified proteins or protein-bioconjugates that are impossible to prepare by standard ribosomal synthesis. In addition to the applications to research tools, there are a growing number of commercial applications of these modified biomolecules, including DNA sequencing, gene SNP analysis, clinical immunoassays, PEGylation of pharmaceutical proteins and so on.
Various techniques to precisely (site specifically) modify proteins with diverse natural and unnatural functionalities have been developed in the last two decades. These methods range from classical bioconjugation reactions to more sophisticated approaches such as the biomimetic transamination reaction, affinity-based protein surface labeling, peptide-/protein-tag fusions, and nonsense suppression mutagenesis. Protein ligation is another powerful protein engineering technique, which allows fully unprotected synthetic and recombinant polypeptides to be regioselectively joined together to build up a target protein molecule. To date, several chemical and enzymatic peptide ligation methods have been reported.
This volume invites original contributions on all aspects of conjugation chemistry and biochemistry, including the preparation, characterization, and chemical and biological properties of molecular conjugates such as antibodies, nucleic acids, lipids, carbohydrates, or other biologically active molecules with any molecular groups that add useful properties (fluorophores, enzymes, drugs, toxins, ligands, etc.). The critical reviews covering both fundamental and applied aspects of this exciting field are also welcome.
Prof. Dr. Teruyuki Nagamune
- biomolecular conjugates
- bioconjugation chemistry
- chemical ligation
- protein ligation
- site-specific chemical and enzymatic modification
Int. J. Mol. Sci. 2012, 13(1), 84-96; doi:10.3390/ijms13010084
Received: 10 October 2011; in revised form: 8 November 2011 / Accepted: 28 November 2011 / Published: 22 December 2011| Download PDF Full-text (185 KB) | Download XML Full-text
Int. J. Mol. Sci. 2012, 13(5), 6053-6072; doi:10.3390/ijms13056053
Received: 5 March 2012; in revised form: 10 April 2012 / Accepted: 9 May 2012 / Published: 18 May 2012| Download PDF Full-text (670 KB) | Download XML Full-text
Last update: 26 February 2014