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Int. J. Mol. Sci. 2012, 13(1), 84-96; doi:10.3390/ijms13010084
Article

Characterization and Comparison of Fumonisin B1-Protein Conjugates by Six Methods

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Received: 10 October 2011; in revised form: 8 November 2011 / Accepted: 28 November 2011 / Published: 22 December 2011
(This article belongs to the Special Issue Protein Conjugates and Bioconjugate Chemistry)
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Abstract: In order to generate an antibody against a small hapten molecule, the hapten is cross-linked with carrier protein to make it immunogenic. In this study, the hapten (Fumonisin B1, FB1) was coupled to ovalbumin (OVA) and bovine serum albumin (BSA), respectively by a short cross-linker reagent (glutaraldehyde, GA). To develop a technique for detecting the conjugation, the hapten-protein conjugates (FB1-OVA and FB1-BSA) were characterized thoroughly by ultraviolet (UV) spectroscopy, Fourier transform infrared (FT-IR) spectroscopy, gel electrophoresis and matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS), respectively. The molecular weights of FB1-BSA and FB1-OVA were 74,355.301 Da and 48,009.212 Da, respectively determined by the method of MALDI-TOF-MS. The molecular coupling ratios were 11 and 5 in FB1-BSA and FB1-OVA, respectively. In this experiment, MALDI-TOF-MS was selected as the most efficient method to evaluate the cross-linking effect and calculate the molecular coupling ratio.
Keywords: Fumonisin B1; conjugated antigen; carrier protein; molar ratio; antibody Fumonisin B1; conjugated antigen; carrier protein; molar ratio; antibody
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Wang, Y.; He, C.-H.; Zheng, H.; Zhang, H.-B. Characterization and Comparison of Fumonisin B1-Protein Conjugates by Six Methods. Int. J. Mol. Sci. 2012, 13, 84-96.

AMA Style

Wang Y, He C-H, Zheng H, Zhang H-B. Characterization and Comparison of Fumonisin B1-Protein Conjugates by Six Methods. International Journal of Molecular Sciences. 2012; 13(1):84-96.

Chicago/Turabian Style

Wang, Ying; He, Cheng-Hua; Zheng, Hao; Zhang, Hai-Bin. 2012. "Characterization and Comparison of Fumonisin B1-Protein Conjugates by Six Methods." Int. J. Mol. Sci. 13, no. 1: 84-96.



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