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Use of Polyphenols: From Green Chemistry to Health

A special issue of International Journal of Molecular Sciences (ISSN 1422-0067). This special issue belongs to the section "Bioactives and Nutraceuticals".

Deadline for manuscript submissions: closed (31 May 2023) | Viewed by 2064

Special Issue Editor


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Guest Editor
1. Department of Analytical Chemistry, Faculty of Sciences, University of Vigo, 32004 Ourense, Spain
2. LAQV-REQUIMTE, Department of Chemistry and Biochemistry, Faculty of Sciences, University of Porto, 4169-007 Porto, Portugal
Interests: quality and food safety; polyphenols; organic chemistry; metab-olomics; proteomics; immune-mediated disorders
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Special Issue Information

Dear Colleagues,

Polyphenols are one of the most relevant families of bioactive compounds naturally present in the Plant Kingdom, which are present in high amounts in food wastes and food by-products. Their high reactivity joined with the wide occurrence and structural diversity makes polyphenols ubiquitous and interesting structures to be upcycled. Under this context, in recent years the use of novel green extraction methods have been raised to obtain polyphenols from food wastes and by-products in a circular economy approach. Likewise, different applications have been studied from cosmetic to pharmaceutic and food industry. However, despite the greater efforts, some gaps need to be filled such as the pharmacokinetics, bioavailability and bioactivities after the incorporation to food or cosmetics, the technological impact and ultimately the global effect on health and socioeconomic impacts. The main aim of this Special issue is to compile research findings in the format of scientific original articles or reviews manuscripts covering the following topics:

  • Polyphenols extraction by green chemistry;
  • Characterization of novel sources of polyphenols;
  • Polyphenols-ligand interactions, polyphenols upcycling and technological impacts.

Dr. María Rosa Pérez-Gregorio
Guest Editor

Manuscript Submission Information

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Keywords

  • polyphenol-ligand interactions
  • green chemistry extraction
  • polyphenols bioactivities

Published Papers (1 paper)

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Research

23 pages, 5052 KiB  
Article
Natural Gallic Acid and Methyl Gallate Induces Apoptosis in Hela Cells through Regulation of Intrinsic and Extrinsic Protein Expression
by Hasmah Abdullah, Ilyana Ismail, Rapeah Suppian and Nor Munirah Zakaria
Int. J. Mol. Sci. 2023, 24(10), 8495; https://doi.org/10.3390/ijms24108495 - 9 May 2023
Cited by 3 | Viewed by 1776
Abstract
Induction of apoptosis is one of the targeted approaches in cancer therapies. As previously reported, natural products can induce apoptosis in in vitro cancer treatments. However, the underlying mechanisms of cancer cell death are poorly understood. The present study aimed to elucidate cell [...] Read more.
Induction of apoptosis is one of the targeted approaches in cancer therapies. As previously reported, natural products can induce apoptosis in in vitro cancer treatments. However, the underlying mechanisms of cancer cell death are poorly understood. The present study aimed to elucidate cell death mechanisms of gallic acid (GA) and methyl gallate (MG) from Quercus infectoria toward human cervical cancer cell lines (HeLa). The antiproliferative activity of GA and MG was characterised by an inhibitory concentration using 50% cell populations (IC50) by an MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide] assay. Cervical cancer cells, HeLa, were treated with GA and MG for 72 h and calculated for IC50 values. The IC50 concentration of both compounds was used to elucidate the apoptotic mechanism using acridine orange/propidium iodide (AO/PI) staining, cell cycle analysis, the Annexin-V FITC dual staining assay, apoptotic proteins expressions (p53, Bax and Bcl-2) and caspase activation analysis. GA and MG inhibited the growth of HeLa cells with an IC50 value of 10.00 ± 0.67 µg/mL and 11.00 ± 0.58 µg/mL, respectively. AO/PI staining revealed incremental apoptotic cells. Cell cycle analysis revealed an accumulation of cells at the sub-G1 phase. The Annexin-V FITC assay showed that cell populations shifted from the viable to apoptotic quadrant. Moreover, p53 and Bax were upregulated, whereas Bcl-2 was markedly downregulated. Activation of caspase 8 and 9 showed an ultimate apoptotic event in HeLa cells treated with GA and MG. In conclusion, GA and MG significantly inhibited HeLa cell growth through apoptosis induction by the activation of the cell death mechanism via extrinsic and extrinsic pathways. Full article
(This article belongs to the Special Issue Use of Polyphenols: From Green Chemistry to Health)
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