The shear constitutive model of the slip zone soil can be used to quantitatively describe the relationship between shear stress and shear displacement, which is of great significance for the analysis of deformation mechanism and stability evaluation of landslides. The conventional shear constitutive models were usually proposed based on statistical damage theory with the Weibull distribution function, which is widely used in the field of rock material. However, there are great differences in the structure and mechanical properties of soil and rock; therefore, the suitability of the damage distribution functions for the slip zone soil needs to be further investigated. In this study, eight distribution functions are introduced to describe the damage evolution process of the slip zone soil and applied to two groups of shear stress–shear displacement curves (named shear curves) with different softening characteristics, i.e., strong softening type and weak softening type. The results show that: (1) the applicability of the various damage distribution functions to the two softening types of shear curves is obviously different; (2) the commonly used Weibull distribution is only suitable for the weak softening shear curves; (3) the shear constitutive models based on Gamma, Exponential, and Logistic distributions are the best three models for the strong softening curve; the shear constitutive models based on Gamma, Weibull, and Exponential distributions are the best three models for the weak softening curve; (4) Gamma distribution function is the optimal model in both strong softening and weak softening types of shear curves, and the parameters of the function have clear physical meaning in the shear constitutive model. In general, the Gamma distribution function can more objectively reflect the whole shear damage evolution process of the slip zone soil than other distribution functions. Full article

Background. Preoperative planning and 3D printing can be used to treat pelvic bone fractures using pre-contoured surgical plates, in particular complex, comminuted fractures involving the acetabulum and quadrilateral plate. The aim of the study was to develop a Fast-Track-Protocol (fast track methodology) for creating 3D anatomical models, that could be used to shape surgical plates, using open-source software and budget 3D printers. Such a ‘low-budget’ approach would allow a hospital-based multidisciplinary team to carry out pre-surgical planning and treat complex pelvic fractures using 3D technology. Methods. The study included 5 patients with comminuted pelvic fractures. For each patient, CT (computed tomography) data were converted into two 3D models of the pelvis-injured side and mirrored model of the contralateral, uninjured hemipelvis. These models were 3D printed and used as templates to shape surgical plates. Results. A Fast-Track-Protocol was established and used to successfully treat 5 patients with complex, comminuted fractures of the pelvis. Conclusion. Using the Fast-Track-Protocol it was possible to prepare 3D printed models and patient-specific pre-contoured plates within 2 days of hospital admittance. Such an approach resulted in better surgical technique and shorter operative times, while incurring relatively low costs. Full article

To combat the threat of antimicrobial resistance, it is important to discover innovative and effective alternative antibacterial agents. Garlic has been recommended as a medicinal plant with antibacterial qualities. Hence, we conducted this study to evaluate the antibacterial activity of ultrasonicated garlic extract against Escherichia coli, Staphylococcus aureus sub. aureus, Streptococcus mutans, and Poryphyromonas gingivalis. Aqueous ultrasonicated garlic extract was tested against these strains, and their antibacterial activity quantified using both agar disk diffusion and agar well diffusion methods; the plate count technique was used to estimate the total viable count. Moreover, Fourier-transform infrared spectroscopy (FTIR), transmission electron microscopy (TEM), and microplate spectrophotometry were used to characterize garlic nanoparticles. The results confirmed that all tested bacteria were sensitive to both sonicated and non-sonicated garlic extracts. Streptococcus mutans was the most susceptible bacteria; on the other hand, Escherichia coli was the most resistant bacteria. Furthermore, characterization of the prepared garlic nanoparticles, showed the presence of organosulfur and phenolic compounds, carboxyl groups, and protein particles. Based on the obtained results, ultrasonicated garlic extract is a potent antibacterial agent. It can come in handy while developing novel antibiotics against bacteria that have developed resistance. Full article

Postoperative tissue regeneration can be negatively affected by bisphosphonate administration, especially in patients with oncologic diseases. A serious complication of bisphosphonate therapy is the medication-related osteonecrosis of the jaw (MRONJ), which can be observed mainly after dental surgery. MRONJ is a progressive destruction of the bone that requires patients to stay in hospital for extended periods of time. For this reason, primary wound closure is particularly important in surgical procedures. In the case of wound dehiscence, there is a very high risk for MRONJ. In recent years, non-invasive physical plasma (NIPP) has become known for improving wound healing on the one hand, but also for its promising efficacy in cancer therapy on the other hand. We report on a 63-year-old patient with a history of multiple myeloma and receiving zoledronate, who developed wound dehiscence after tooth extraction. NIPP treatment resulted in complete epithelialization of the entire wound dehiscence. In conclusion, the use of NIPP in patients receiving antiresorptive drugs seems to support tissue regeneration and thus could be an important tool for the prevention of MRONJ. Full article

The size effect is a phenomenon where the strength and the ductility of a material depend on the size of the structure. Investigating size effects and related crack formation in brittle materials requires advanced monitoring methods. The aim of this research is to experimentally investigate the impact of size effect with the acoustic emission (AE) technique. Brazilian splitting tests with AE monitoring were performed on cement-based mortar cylinders of three sizes. It was found that in addition to the size, the boundary condition affects the final strength. When adopting similar boundary conditions in samples with different sizes, the larger samples had the lowest tensile splitting strength. For the larger samples, initially, there were fewer AE activities. However, there was a surge of high-amplitude AE events near the peak load. This indicates that as size increases, there is a lack of micro-cracking before macro-crack propagation, and the material fails in a more brittle manner. The width of the fracture process zone was quantified with AE and increased with sample size. A further analysis of the AE amplitude distribution demonstrated a change in the distribution in the pre-peak phase for the larger samples and for the smaller samples in the post-peak phase, signifying the brittle to ductile failure transition that occurs as size decreases. Full article

The effects of pH (3.6 and 7.0) and irradiation UV-C dose irradiation (0, 9.81, 15.13, and 31.87 mJ/cm²) on the physicochemical properties and natural microbiota of red prickly pear juice were evaluated during processing and storage. Thermal treatments were used as the control applying high temperatures for a short time (HTST 80 °C/30 s) or ultra-high temperature (UHT 130 °C/3 s). UV-C treatments applied to juices with both pHs inactivated coliforms and mesophiles with the same efficacy as thermal treatments. Yeasts and molds were inactivated at a dose of >15.13 mJ/cm² at both pHs. The UV-C doses showed no differences in betalains, polyphenols, or antioxidant activity. However, a decrease in these compounds was observed during storage. The lowest reductions in betacyanins (11.1–16.7%) and betaxanthins (2.38–10.22%) were obtained by UV-C treatment at pH 3.6. Thermal treatments (HTST and UHT) caused a reduction greater than UV-C irradiation in betacyanins, betaxanthins, polyphenols, and antioxidant activity after treatment. However, after storage at pH 3.6, the contents of these compounds reached those of the UV-C treatments, except for polyphenols. In specific pigments, betanin retention was highest at pH 3.6 (62.26–87.24%), and its retention decreases with UV-C dose increase and storage. The indicaxanthin retentions were higher (75.85–92.27%) than those of betanin, and the reduction was mainly due to storage. The physical properties (pH, acidity, and °Brix) were not affected by treatments, except for the color. The results suggest that a dose of 15.13 mJ/cm² of a continuous UV-C system is a non-thermal alternative for the processing of red prickly pear juice at pH 3.6, preserving its properties. Full article

Xanthine oxidase (XO) plays an important role in purine degradation in humans. The study aimed to determine the XO inhibitory potential of Chrysanthemum morifolium dried flower ethyl acetate sub-fractions and its anti-hyperuricemic effect in rat models. Bioassay-guided fractionation based on XO inhibitory assay was employed to obtain bioactive fractions and sub-fractions. In vitro cytotoxicity and cellular antioxidant capacity of the sub-fraction and its mode of XO inhibition were also investigated. The anti-hyperuricemic effect of the bioactive sub-fraction was investigated using rat models via oral consumption, and followed by an XO mRNA gene expression study. The compounds in the bioactive sub-fractions were identified putatively using HPLC-Q-TOF-MS/MS. Ethyl acetate (EtOAc) fraction exhibited the highest XO inhibition among the fractions. It was further fractionated into 15 sub-fractions. F10 exhibited high XO inhibitory activity, cellular pro-proliferative effect, and intracellular antioxidant activity among the sub-fractions tested. This sub-fraction was non-cytotoxic at 0.1–10 µg/mL, and very effective in lowering serum and urine uric acid level in rat models upon oral consumption. A total of 26 known compounds were identified and seven unknown compounds were detected via HPLC-Q-TOF–MS/MS analysis. The possible mechanisms contributing to the anti-hyperuricemic effect were suggested to be the non-competitive inhibition of XO enzyme, XO gene expression down-regulation, and the enhancement of uric acid excretion. Full article

Reflective cracking is one of the major forms of deterioration in semi-rigid base asphalt pavements. It is, therefore, very important to have a correct understanding of the internal crack propagation mechanism of asphalt pavement to propose the most effective remedial solution(s), which corresponds to that mode of failure. In this study, two-dimensional asphalt pavement layered models are first established by modifying the peridynamics theory. Then, the influence of asphalt overlay thickness and load form on reflective crack propagation is explored. On this basis, the influence of friction between the tire and road surface on reflective crack propagation is analyzed. The results show that increasing the thickness of the asphalt overlay can inhibit reflective crack propagation, and the friction force accelerates reflective crack propagation when the direction of friction is the same as that of reflective crack propagation; otherwise, it inhibits reflective crack propagation. Additionally, the most unfavorable load position is the asymmetrical load when the vehicle is far from the reflective crack. Full article

In Molecular Tumor Boards, a team of experts discuss the individual therapy options of a cancer patient based on their individual molecular profile. The process—from recommendation request, through molecular diagnosis, to a personalized therapy recommendation—is complex and time-consuming. Therefore, process optimization is needed to decrease the workload of physicians and to standardize the process. For this purpose, we modeled the current workflow of the Molecular Tumor Board at the University Hospital Hamburg-Eppendorf on Service-Oriented Architecture using Business Process Modeling and Notation to highlight areas for improvement. This identified many manual tasks and an extensive workload for the physician. We then created a novel, simplified, more efficient workflow in which the physician is supported by additional software. In summary, we show that the use of Service-Oriented Architecture using Business Process Modeling and Notation for Molecular Tumor Board processes promotes rapid adaptability, standardization, interoperability, quality assurance, and facilitates collaboration. Full article

Heart rate variability (HRV) is a known measure of cardiac autonomic function. A cardiovascular autonomic dysfunction (CAD), measured as changes in HRV, is usually presented after an infectious process. The aim of the present study is to assess the association between serum inflammatory markers and CAD. For this purpose, 50 volunteers (13 of them recovering from an infection) were recruited and followed-up for 6 weeks. Their serum inflammatory biomarkers (CRP, IL1, IL4, IL6, IL10, and TNFalpha) were quantified throughout those weeks, along with their HRV resting, in response to the Valsalva maneuver, metronome breathing, standing and sustained handgrip. The correlation of within-subject changes in both HRV and inflammatory biomarkers was assessed to evaluate the concurrent changes. An inverse within-subject correlation was found between CRP and HRV in response to the Valsalva maneuver (rho (95% CI): −0.517 (−0.877 to −0.001); p = 0.032) and HRV standing (rho (95% CI): −0.490 (−0.943 to −0.036); p = 0.034). At the beginning, increased values of CRP are found along with reduced levels of HRV. Then, the CRP was reduced, accompanied by an improvement (increase) in HRV. These results suggest that CRP is a potential marker of CAD. Whether it is the cause, the consequence or a risk indicator non-causally associated is still to be determined. Full article

Many commercial strains of the Saccharomyces cerevisiae species are used around the world in the wine industry, while the use of native yeast strains is highly recommended for their role in shaping specific, terroir-associated wine characteristics. In recent years, in Poland, an increase in the number of registered vineyards has been observed, and Polish wines are becoming more recognizable among consumers. In the fermentation process, apart from ethyl alcohol, numerous microbial metabolites are formed. These compounds shape the wine bouquet or become precursors for the creation of new products that affect the sensory characteristics and quality of the wine. The aim of this work was to study the effect of the grapevine varieties and newly isolated native S. cerevisiae yeast strains on the content of selected wine fermentation metabolites. Two vine varieties—Regent and Seyval blanc were used. A total of 16 different yeast strains of the S. cerevisiae species were used for fermentation: nine newly isolated from vine fruit and seven commercial cultures. The obtained wines differed in terms of the content of analyzed oenological characteristics and the differences depended both on the raw material (vine variety) as well as the source of isolation and origin of the yeast strain used (commercial vs. native). Generally, red wines characterized a higher content of tested analytes than white wines, regardless of the yeast strain used. The red wines are produced with the use of native yeast strains characterized by higher content of amyl alcohols and esters. Full article

Evolutionary algorithms have been widely used to solve complex engineering optimization problems with large search spaces and nonlinearity. Both cultural algorithm (CA) and genetic algorithms (GAs) have a broad prospect in the optimization field. The traditional CA has poor precision in solving complex engineering optimization problems and easily falls into local optima. An efficient hybrid evolutionary optimization method coupling CA with GAs (HCGA) is proposed in this paper. HCGA reconstructs the cultural framework, which uses three kinds of knowledge to build the belief space, and the GAs are used as an evolutionary model for the population space. In addition, a knowledge-guided t-mutation operator is developed to dynamically adjust the mutation step and introduced into the influence function. HCGA achieves a balance between exploitation and exploration through the above strategies, and thus effectively avoids falling into local optima and improves the optimization efficiency. Numerical experiments and comparisons with several benchmark functions show that the proposed HCGA significantly outperforms the other compared algorithms in terms of comprehensive performance, especially for high-dimensional problems. HCGA is further applied to aerodynamic optimization design, with the wing cruise factor being improved by 23.21%, demonstrating that HCGA is an efficient optimization algorithm with potential applications in aerodynamic optimization design. Full article

Isoflavone glycosides are commonly biotransformed into isoflavone aglycones due to the superior biological activities of the latter. Wild soybeans contain a higher isoflavone content than domesticated soybeans due to their high level of genetic diversity. In this study, we cloned and characterized a thermostable β-galactosidase from the extreme thermophile Thermoproteus uzoniensis for potential application in isoflavone conversion in Korean wild soybeans. The purified recombinant enzyme exhibited a maximum specific activity of 1103 μmol/min/mg at pH 5.0 and 90 °C with a half-life of 46 h and exists as a homodimer of 113 kDa. The enzyme exhibited the highest activity for p-nitrophenyl (pNP)-β-D-galactopyranoside among aryl glycosides and it hydrolyzed isoflavone glycosides in the order genistin > daidzin > ononin > glycitin. The enzyme completely hydrolyzed 2.77 mM daidzin and 3.85 mM genistin in the seed extract of wild soybean after 80 and 70 min with productivities of 1.86 and 3.30 mM/h, respectively, and 9.89 mM daidzin and 1.67 mM genistin in the root extract after 180 and 30 min, with the highest productivities of 3.30 and 3.36 mM/h, respectively, compared to other glycosidases. Our results will contribute to the industrial production of isoflavone aglycone using wild soybean and this is the first report on the enzymatic production of isoflavone aglycones from isoflavone glycosides in wild soybeans. Full article

In the process of power transformer risk assessment, the loss degree index is difficult to accurately quantify due to the influence of uncertain factors, leading to the deviation of risk judgment. A power transformer risk assessment method based on the three-parameter interval grey number decision-making is proposed. Firstly, the fault probability of the transformer is quantified based on the condition evaluation results. Secondly, considering the uncertainty of DG output and load, the Nataf transform and Cholesky decomposition were used to eliminate the correlation of random variables, and a three-point estimation method combined with a DC cut load model was introduced to calculate the probability distribution of the loss degree caused by the transformer fault. Finally, the origin moment of each order was obtained based on the calculation formula of risk value, and the risk probability distribution was obtained through the Cornish–Fisher series expanding. The decision method of the three-parameter interval grey number distance measure was used to judge the risk grade of the equipment. The results show that the proposed method fully considers the influence of uncertainty on equipment risk judgment, can realize the full use of the equipment risk value interval number to judge the risk, and avoids the decision-making defects of the traditional certain risk quantification method. Meanwhile, the influence of different factors on the risk evaluation results is in line with the actual operation condition of the transformer. The results also verify the effectiveness and accuracy of the proposed method, which provides a new judgment idea for power grid equipment risk quantitative assessment. Full article

Active methods are proposed to improve the measurement accuracy of a compact laser diode-based (LD-based) system, which is designed to measure the geometric errors of machine tools. The LD has some advantages, such as a small size, low cost and high efficiency. However, the laser spot of the LD is elliptical and the stability in the output power of the LD is low, which limits the accuracy of the measurement system, where the LD is used as the laser source. An active shaping method is proposed to shape the elliptical laser spot of the LD without adding additional optical elements. In addition, the laser beam drifts, including the linear drift and angular drift, are compensated in real-time by a proposed improved active error compensator, which consists of two drift feedback units and a Backpropagation Neural Networks-based PID controller, during the long-distance measurement. A series of experiments were conducted to verify the effectiveness of the proposed methods and the capability of the constructed LD-based system. Full article