Special Issue "Dynamic Approaches to Metabolic Modeling and Metabolic Engineering"

A special issue of Processes (ISSN 2227-9717).

Deadline for manuscript submissions: closed (30 June 2014)

Special Issue Editor

Guest Editor
Prof. Dr. Doraiswami Ramkrishna

Forney Hall of Chemical Engineering, 480 Stadium Mall Drive, Purdue University, West Lafayette, IN 47907, USA
Website | E-Mail
Fax: +1 765 494 0805
Interests: metabolic modeling; metabolic engineering; particulate processes; population balances

Special Issue Information

Dear Colleagues,

Processes (ISSN 2227-9717), an international open access journal on processes in chemistry, biochemistry, biology, and related engineering research fields, is published by MDPI online quarterly. For more information, please refer to Processes' Aims and Scope at: http://www.mdpi.com/journal/processes/about. This Special Issue of Processes is expected to gather contributions in the general area of Dynamic Approaches to Metabolic Modeling and Metabolic Engineering.

Overall, this Special Issue is dedicated to applying mathematical models towards the translation of fundamental theory to design, optimize and control engineering systems. Specifically, it aims to promote the use of dynamic modeling from metabolic networks to metabolic engineering, then further to enable the design of organisms to maximize productivity.

While successful case studies will be of special interest, we also welcome papers that deliberate on the methodology, irrespective of whether the stipulated engineering goals have been reached successfully.

Prof. Dr. Doraiswami Ramkrishna
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All papers will be peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Processes is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 350 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.


Keywords

  • metabolic networks
  • metabolic Engineering
  • genetic Engineering
  • mutation
  • gene knockout
  • gene insertion
  • product yield and productivity

Published Papers (1 paper)

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Research

Open AccessArticle Dynamic Modeling of Cell-Free Biochemical Networks Using Effective Kinetic Models
Processes 2015, 3(1), 138-160; doi:10.3390/pr3010138
Received: 8 September 2014 / Revised: 16 February 2015 / Accepted: 17 February 2015 / Published: 3 March 2015
Cited by 5 | PDF Full-text (3563 KB) | HTML Full-text | XML Full-text
Abstract
Cell-free systems offer many advantages for the study, manipulation and modeling of metabolism compared to in vivo processes. Many of the challenges confronting genome-scale kinetic modeling can potentially be overcome in a cell-free system. For example, there is no complex transcriptional regulation to
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Cell-free systems offer many advantages for the study, manipulation and modeling of metabolism compared to in vivo processes. Many of the challenges confronting genome-scale kinetic modeling can potentially be overcome in a cell-free system. For example, there is no complex transcriptional regulation to consider, transient metabolic measurements are easier to obtain, and we no longer have to consider cell growth. Thus, cell-free operation holds several significant advantages for model development, identification and validation. Theoretically, genome-scale cell-free kinetic models may be possible for industrially important organisms, such as E. coli, if a simple, tractable framework for integrating allosteric regulation with enzyme kinetics can be formulated. Toward this unmet need, we present an effective biochemical network modeling framework for building dynamic cell-free metabolic models. The key innovation of our approach is the integration of simple effective rules encoding complex allosteric regulation with traditional kinetic pathway modeling. We tested our approach by modeling the time evolution of several hypothetical cell-free metabolic networks. We found that simple effective rules, when integrated with traditional enzyme kinetic expressions, captured complex allosteric patterns such as ultrasensitivity or non-competitive inhibition in the absence of mechanistic information. Second, when integrated into network models, these rules captured classic regulatory patterns such as product-induced feedback inhibition. Lastly, we showed, at least for the network architectures considered here, that we could simultaneously estimate kinetic parameters and allosteric connectivity from synthetic data starting from an unbiased collection of possible allosteric structures using particle swarm optimization. However, when starting with an initial population that was heavily enriched with incorrect structures, our particle swarm approach could converge to an incorrect structure. While only an initial proof-of-concept, the framework presented here could be an important first step toward genome-scale cell-free kinetic modeling of the biosynthetic capacity of industrially important organisms. Full article
(This article belongs to the Special Issue Dynamic Approaches to Metabolic Modeling and Metabolic Engineering)
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