Evaluation of Quality Traits of Crop Resources and Germplasm Innovation

A special issue of Life (ISSN 2075-1729). This special issue belongs to the section "Plant Science".

Deadline for manuscript submissions: closed (16 February 2023) | Viewed by 3341

Special Issue Editors

Institute of Crop Science, Chinese Academy of Agricultural Sciences, No.80 South Xueyuan Road, Haidian District, Beijing 100081, China
Interests: crop germplasm; grain quality; food chemistry

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Guest Editor
Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, No. 348 Xianjiahu West Road, Changsha 410205, China
Interests: identification of functional ingredients from plant sources; bioactivity evaluation; functional food development
Special Issues, Collections and Topics in MDPI journals
Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China
Interests: crop germplasm; gene discovery; plant genome
Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China
Interests: cereal nutrition; risk factors in crops; quality safety and risk assessment

Special Issue Information

Dear Colleagues,

Crop germplasm, the material basis for crop breeding, is a strategic resource for the survival and development of human society. The occurrence of the development of social economics, population aging, and metabolic disease increasing all over the world, have promoted the need for high-quality food with healthy benefits. Therefore, the quality improvement has become one of the main objectives in crop breeding. Moreover, evaluation of quality traits and germplasm innovation are the basis for development of high-quality crop variety and important parts of crop germplasm research.

In recent years, as the rapid development of technologies of spectrum, chromatography, and mass spectrometry; molecular biology technology; multi-omics technology; artificial intelligence and information technology (big data and analytics); and research on evaluation of quality traits and germplasm innovation has entered a new stage. For instance, diversified, rapid, non-destructive, high-throughput, automated, and informationized techniques and models have been widely employed in the evaluation of quality traits of crop germplasms. The evaluation of quality traits has extended from nutritional quality to functional, processing, sensory, and others qualities (forage, ornamental, etc.). The technology of gene editing (e.g., CRISPR) is already used in germplasm innovation.

In this Research Topic, we welcome the submission of original research papers and reviews that cover wide research topics, including, but not limited to, the following:

  • Identification and evaluation of quality traits of crop germplasm resources;
  • Genetic diversity analysis of quality traits of crop germplasm resources;
  • Effect of genotype–environment interactions on quality traits of crop germplasm resources;
  • Innovation and utilization of high-quality germplasm;
  • Methodological study: development or improvement of the methods of quality traits evaluation as well as germplasm innovation.

Dr. Peiyou Qin
Dr. Xiushi Yang
Dr. Jing Wu
Dr. Li Wu
Guest Editors

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Keywords

  • crop germplasm
  • quality traits
  • identification
  • evaluation
  • germplasm innovation
  • genetic diversity
  • genotype-environment interactions

Published Papers (1 paper)

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Research

15 pages, 2582 KiB  
Article
Novel PCR-Based Multiplex Assays for Detecting Major Quality and Biotic Stress in Commercial and Weedy Rice
by Noraikim Mohd Hanafiah, Acga Cheng, Phaik-Eem Lim, Gomathy Sethuraman, Nurul Amalina Mohd Zain, Niranjan Baisakh and Muhamad Shakirin Mispan
Life 2022, 12(10), 1542; https://doi.org/10.3390/life12101542 - 4 Oct 2022
Cited by 2 | Viewed by 2402
Abstract
While previous research has demonstrated that multiplex polymerase chain reaction (PCR) can be a cost-effective approach to detect various genes in crops, the availability of multiplex assays to simultaneously screen both grain quality and biotic stress resistance traits in rice (Oryza sativa [...] Read more.
While previous research has demonstrated that multiplex polymerase chain reaction (PCR) can be a cost-effective approach to detect various genes in crops, the availability of multiplex assays to simultaneously screen both grain quality and biotic stress resistance traits in rice (Oryza sativa) is limited. In this work, we report six novel multiplex assays that use a universal protocol to detect major rice grain quality (amylose content and fragrance) and biotic stress (blast, sheath blight, and bacterial leaf blight) traits with amplified products consisting of up to four primer pairs that can be analyzed using a standard agarose-based gel electrophoresis system. Recent studies have suggested that weedy rice has novel sources of disease resistance. However, an intensive screening of weedy biotypes has not been reported in Malaysia. Accordingly, we employed one of the developed multiplex assays to screen reported genes or quantitative trait loci (QTLs) associated with blast, sheath blight, and bacterial leaf blight diseases in 100 weedy rice biotypes collected from five local fields, with phenotyping performed to validate the genotyping results. In conclusion, our universal multiplex protocol is effective for the large-scale genotyping of rice genetic resources, and it can be employed in routine molecular laboratories with limited resources. Full article
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